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Rapid screening of high expressing Escherichia coli colonies using a novel dicistronic-autoinducible system

BACKGROUND: Identification of high-expressing colonies is one of the main concerns in the upstream process of recombinant protein development. The common method to screen high-producing colonies is SDS-PAGE, a laborious and time-consuming process, which is based on a random and qualitative way. The...

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Detalles Bibliográficos
Autores principales: Shariati, Fatemeh Sadat, Norouzian, Dariush, Valizadeh, Vahideh, Ahangari Cohan, Reza, Keramati, Malihe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8666062/
https://www.ncbi.nlm.nih.gov/pubmed/34895227
http://dx.doi.org/10.1186/s12934-021-01711-2