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Rapid screening of high expressing Escherichia coli colonies using a novel dicistronic-autoinducible system
BACKGROUND: Identification of high-expressing colonies is one of the main concerns in the upstream process of recombinant protein development. The common method to screen high-producing colonies is SDS-PAGE, a laborious and time-consuming process, which is based on a random and qualitative way. The...
Autores principales: | Shariati, Fatemeh Sadat, Norouzian, Dariush, Valizadeh, Vahideh, Ahangari Cohan, Reza, Keramati, Malihe |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8666062/ https://www.ncbi.nlm.nih.gov/pubmed/34895227 http://dx.doi.org/10.1186/s12934-021-01711-2 |
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