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Ionophore application for artificial oocyte activation and its potential effect on morphokinetics: a sibling oocyte study
PURPOSE: To evaluate whether ionophore application at the oocyte stage changes the morphokinetics of the associated embryos in cases of artificial oocyte activation. METHODS: In a prospective sibling oocyte approach, 78 ICSI patients with suspected fertilization problems had half of their MII-oocyte...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8666403/ https://www.ncbi.nlm.nih.gov/pubmed/34642877 http://dx.doi.org/10.1007/s10815-021-02338-3 |
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author | Shebl, Omar Trautner, Philip Sebastian Enengl, Sabine Reiter, Elisabeth Allerstorfer, Christina Rechberger, Tamara Oppelt, Peter Ebner, Thomas |
author_facet | Shebl, Omar Trautner, Philip Sebastian Enengl, Sabine Reiter, Elisabeth Allerstorfer, Christina Rechberger, Tamara Oppelt, Peter Ebner, Thomas |
author_sort | Shebl, Omar |
collection | PubMed |
description | PURPOSE: To evaluate whether ionophore application at the oocyte stage changes the morphokinetics of the associated embryos in cases of artificial oocyte activation. METHODS: In a prospective sibling oocyte approach, 78 ICSI patients with suspected fertilization problems had half of their MII-oocytes treated with a ready-to-use ionophore (calcimycin) immediately following ICSI (study group). Untreated ICSI eggs served as the control group. Primary analyses focused on morphokinetic behavior and the presence of irregular cleavages. The rates of fertilization, utilization, pregnancy, and live birth rate were also evaluated. RESULTS: Ionophore-treated oocytes showed a significantly earlier formation of pronuclei (t2PNa) and a better synchronized third cell cycle (s3) (P < .05). The rate of irregular cleavage was unaffected (P > .05). Ionophore treatment significantly improved the overall rates of fertilization (P < .01) and blastocyst utilization (P < .05). CONCLUSION: Ionophore application does not negatively affect cleavage timing nor is it associated with irregular cleavage. |
format | Online Article Text |
id | pubmed-8666403 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-86664032021-12-27 Ionophore application for artificial oocyte activation and its potential effect on morphokinetics: a sibling oocyte study Shebl, Omar Trautner, Philip Sebastian Enengl, Sabine Reiter, Elisabeth Allerstorfer, Christina Rechberger, Tamara Oppelt, Peter Ebner, Thomas J Assist Reprod Genet Embryo Biology PURPOSE: To evaluate whether ionophore application at the oocyte stage changes the morphokinetics of the associated embryos in cases of artificial oocyte activation. METHODS: In a prospective sibling oocyte approach, 78 ICSI patients with suspected fertilization problems had half of their MII-oocytes treated with a ready-to-use ionophore (calcimycin) immediately following ICSI (study group). Untreated ICSI eggs served as the control group. Primary analyses focused on morphokinetic behavior and the presence of irregular cleavages. The rates of fertilization, utilization, pregnancy, and live birth rate were also evaluated. RESULTS: Ionophore-treated oocytes showed a significantly earlier formation of pronuclei (t2PNa) and a better synchronized third cell cycle (s3) (P < .05). The rate of irregular cleavage was unaffected (P > .05). Ionophore treatment significantly improved the overall rates of fertilization (P < .01) and blastocyst utilization (P < .05). CONCLUSION: Ionophore application does not negatively affect cleavage timing nor is it associated with irregular cleavage. Springer US 2021-10-13 2021-12 /pmc/articles/PMC8666403/ /pubmed/34642877 http://dx.doi.org/10.1007/s10815-021-02338-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Embryo Biology Shebl, Omar Trautner, Philip Sebastian Enengl, Sabine Reiter, Elisabeth Allerstorfer, Christina Rechberger, Tamara Oppelt, Peter Ebner, Thomas Ionophore application for artificial oocyte activation and its potential effect on morphokinetics: a sibling oocyte study |
title | Ionophore application for artificial oocyte activation and its potential effect on morphokinetics: a sibling oocyte study |
title_full | Ionophore application for artificial oocyte activation and its potential effect on morphokinetics: a sibling oocyte study |
title_fullStr | Ionophore application for artificial oocyte activation and its potential effect on morphokinetics: a sibling oocyte study |
title_full_unstemmed | Ionophore application for artificial oocyte activation and its potential effect on morphokinetics: a sibling oocyte study |
title_short | Ionophore application for artificial oocyte activation and its potential effect on morphokinetics: a sibling oocyte study |
title_sort | ionophore application for artificial oocyte activation and its potential effect on morphokinetics: a sibling oocyte study |
topic | Embryo Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8666403/ https://www.ncbi.nlm.nih.gov/pubmed/34642877 http://dx.doi.org/10.1007/s10815-021-02338-3 |
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