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Ionophore application for artificial oocyte activation and its potential effect on morphokinetics: a sibling oocyte study

PURPOSE: To evaluate whether ionophore application at the oocyte stage changes the morphokinetics of the associated embryos in cases of artificial oocyte activation. METHODS: In a prospective sibling oocyte approach, 78 ICSI patients with suspected fertilization problems had half of their MII-oocyte...

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Autores principales: Shebl, Omar, Trautner, Philip Sebastian, Enengl, Sabine, Reiter, Elisabeth, Allerstorfer, Christina, Rechberger, Tamara, Oppelt, Peter, Ebner, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8666403/
https://www.ncbi.nlm.nih.gov/pubmed/34642877
http://dx.doi.org/10.1007/s10815-021-02338-3
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author Shebl, Omar
Trautner, Philip Sebastian
Enengl, Sabine
Reiter, Elisabeth
Allerstorfer, Christina
Rechberger, Tamara
Oppelt, Peter
Ebner, Thomas
author_facet Shebl, Omar
Trautner, Philip Sebastian
Enengl, Sabine
Reiter, Elisabeth
Allerstorfer, Christina
Rechberger, Tamara
Oppelt, Peter
Ebner, Thomas
author_sort Shebl, Omar
collection PubMed
description PURPOSE: To evaluate whether ionophore application at the oocyte stage changes the morphokinetics of the associated embryos in cases of artificial oocyte activation. METHODS: In a prospective sibling oocyte approach, 78 ICSI patients with suspected fertilization problems had half of their MII-oocytes treated with a ready-to-use ionophore (calcimycin) immediately following ICSI (study group). Untreated ICSI eggs served as the control group. Primary analyses focused on morphokinetic behavior and the presence of irregular cleavages. The rates of fertilization, utilization, pregnancy, and live birth rate were also evaluated. RESULTS: Ionophore-treated oocytes showed a significantly earlier formation of pronuclei (t2PNa) and a better synchronized third cell cycle (s3) (P < .05). The rate of irregular cleavage was unaffected (P > .05). Ionophore treatment significantly improved the overall rates of fertilization (P < .01) and blastocyst utilization (P < .05). CONCLUSION: Ionophore application does not negatively affect cleavage timing nor is it associated with irregular cleavage.
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spelling pubmed-86664032021-12-27 Ionophore application for artificial oocyte activation and its potential effect on morphokinetics: a sibling oocyte study Shebl, Omar Trautner, Philip Sebastian Enengl, Sabine Reiter, Elisabeth Allerstorfer, Christina Rechberger, Tamara Oppelt, Peter Ebner, Thomas J Assist Reprod Genet Embryo Biology PURPOSE: To evaluate whether ionophore application at the oocyte stage changes the morphokinetics of the associated embryos in cases of artificial oocyte activation. METHODS: In a prospective sibling oocyte approach, 78 ICSI patients with suspected fertilization problems had half of their MII-oocytes treated with a ready-to-use ionophore (calcimycin) immediately following ICSI (study group). Untreated ICSI eggs served as the control group. Primary analyses focused on morphokinetic behavior and the presence of irregular cleavages. The rates of fertilization, utilization, pregnancy, and live birth rate were also evaluated. RESULTS: Ionophore-treated oocytes showed a significantly earlier formation of pronuclei (t2PNa) and a better synchronized third cell cycle (s3) (P < .05). The rate of irregular cleavage was unaffected (P > .05). Ionophore treatment significantly improved the overall rates of fertilization (P < .01) and blastocyst utilization (P < .05). CONCLUSION: Ionophore application does not negatively affect cleavage timing nor is it associated with irregular cleavage. Springer US 2021-10-13 2021-12 /pmc/articles/PMC8666403/ /pubmed/34642877 http://dx.doi.org/10.1007/s10815-021-02338-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Embryo Biology
Shebl, Omar
Trautner, Philip Sebastian
Enengl, Sabine
Reiter, Elisabeth
Allerstorfer, Christina
Rechberger, Tamara
Oppelt, Peter
Ebner, Thomas
Ionophore application for artificial oocyte activation and its potential effect on morphokinetics: a sibling oocyte study
title Ionophore application for artificial oocyte activation and its potential effect on morphokinetics: a sibling oocyte study
title_full Ionophore application for artificial oocyte activation and its potential effect on morphokinetics: a sibling oocyte study
title_fullStr Ionophore application for artificial oocyte activation and its potential effect on morphokinetics: a sibling oocyte study
title_full_unstemmed Ionophore application for artificial oocyte activation and its potential effect on morphokinetics: a sibling oocyte study
title_short Ionophore application for artificial oocyte activation and its potential effect on morphokinetics: a sibling oocyte study
title_sort ionophore application for artificial oocyte activation and its potential effect on morphokinetics: a sibling oocyte study
topic Embryo Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8666403/
https://www.ncbi.nlm.nih.gov/pubmed/34642877
http://dx.doi.org/10.1007/s10815-021-02338-3
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