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Identification of Potential circRNA-microRNA-mRNA Regulatory Network in Skeletal Muscle

Circular RNAs (circRNAs) are a newly discovered family of regulatory RNAs generated through backsplicing. Genome-wide profiling of circRNAs found that circRNAs are ubiquitously expressed and regulate gene expression by acting as a sponge for RNA-binding proteins (RBPs) and microRNAs (miRNAs). To ide...

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Autores principales: Das, Arundhati, Shyamal, Sharmishtha, Sinha, Tanvi, Mishra, Smruti Sambhav, Panda, Amaresh C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8666571/
https://www.ncbi.nlm.nih.gov/pubmed/34912845
http://dx.doi.org/10.3389/fmolb.2021.762185
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author Das, Arundhati
Shyamal, Sharmishtha
Sinha, Tanvi
Mishra, Smruti Sambhav
Panda, Amaresh C.
author_facet Das, Arundhati
Shyamal, Sharmishtha
Sinha, Tanvi
Mishra, Smruti Sambhav
Panda, Amaresh C.
author_sort Das, Arundhati
collection PubMed
description Circular RNAs (circRNAs) are a newly discovered family of regulatory RNAs generated through backsplicing. Genome-wide profiling of circRNAs found that circRNAs are ubiquitously expressed and regulate gene expression by acting as a sponge for RNA-binding proteins (RBPs) and microRNAs (miRNAs). To identify circRNAs expressed in mouse skeletal muscle, we performed high-throughput RNA-sequencing of circRNA-enriched gastrocnemius muscle RNA samples, which identified more than 1,200 circRNAs. In addition, we have identified more than 14,000 and 15,000 circRNAs in aging human skeletal muscle tissue and satellite cells, respectively. A subset of abundant circRNAs was analyzed by RT-PCR, Sanger sequencing, and RNase R digestion assays to validate their expression in mouse skeletal muscle tissues. Analysis of the circRNA-miRNA-mRNA regulatory network revealed that conserved circNfix might associate with miR-204-5p, a suppressor of myocyte enhancer factor 2c (Mef2c) expression. To support the hypothesis that circNfix might regulate myogenesis by controlling Mef2c expression, silencing circNfix moderately reduced Mef2c mRNA expression and inhibited C2C12 differentiation. We propose that circNfix promotes MEF2C expression during muscle cell differentiation in part by acting as a sponge for miR-204-5p.
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spelling pubmed-86665712021-12-14 Identification of Potential circRNA-microRNA-mRNA Regulatory Network in Skeletal Muscle Das, Arundhati Shyamal, Sharmishtha Sinha, Tanvi Mishra, Smruti Sambhav Panda, Amaresh C. Front Mol Biosci Molecular Biosciences Circular RNAs (circRNAs) are a newly discovered family of regulatory RNAs generated through backsplicing. Genome-wide profiling of circRNAs found that circRNAs are ubiquitously expressed and regulate gene expression by acting as a sponge for RNA-binding proteins (RBPs) and microRNAs (miRNAs). To identify circRNAs expressed in mouse skeletal muscle, we performed high-throughput RNA-sequencing of circRNA-enriched gastrocnemius muscle RNA samples, which identified more than 1,200 circRNAs. In addition, we have identified more than 14,000 and 15,000 circRNAs in aging human skeletal muscle tissue and satellite cells, respectively. A subset of abundant circRNAs was analyzed by RT-PCR, Sanger sequencing, and RNase R digestion assays to validate their expression in mouse skeletal muscle tissues. Analysis of the circRNA-miRNA-mRNA regulatory network revealed that conserved circNfix might associate with miR-204-5p, a suppressor of myocyte enhancer factor 2c (Mef2c) expression. To support the hypothesis that circNfix might regulate myogenesis by controlling Mef2c expression, silencing circNfix moderately reduced Mef2c mRNA expression and inhibited C2C12 differentiation. We propose that circNfix promotes MEF2C expression during muscle cell differentiation in part by acting as a sponge for miR-204-5p. Frontiers Media S.A. 2021-11-29 /pmc/articles/PMC8666571/ /pubmed/34912845 http://dx.doi.org/10.3389/fmolb.2021.762185 Text en Copyright © 2021 Das, Shyamal, Sinha, Mishra and Panda. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Das, Arundhati
Shyamal, Sharmishtha
Sinha, Tanvi
Mishra, Smruti Sambhav
Panda, Amaresh C.
Identification of Potential circRNA-microRNA-mRNA Regulatory Network in Skeletal Muscle
title Identification of Potential circRNA-microRNA-mRNA Regulatory Network in Skeletal Muscle
title_full Identification of Potential circRNA-microRNA-mRNA Regulatory Network in Skeletal Muscle
title_fullStr Identification of Potential circRNA-microRNA-mRNA Regulatory Network in Skeletal Muscle
title_full_unstemmed Identification of Potential circRNA-microRNA-mRNA Regulatory Network in Skeletal Muscle
title_short Identification of Potential circRNA-microRNA-mRNA Regulatory Network in Skeletal Muscle
title_sort identification of potential circrna-microrna-mrna regulatory network in skeletal muscle
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8666571/
https://www.ncbi.nlm.nih.gov/pubmed/34912845
http://dx.doi.org/10.3389/fmolb.2021.762185
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