Process control and in silico modeling strategies for enabling high density culture of human pluripotent stem cells in stirred tank bioreactors

The routine therapeutic and industrial applications of human pluripotent stem cells (hPSCs) require their constant mass supply by robust, efficient, and economically viable bioprocesses. Our protocol describes the fully controlled expansion of hPSCs in stirred tank bioreactors (STBRs) enabling cell...

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Detalles Bibliográficos
Autores principales: Manstein, Felix, Ullmann, Kevin, Triebert, Wiebke, Zweigerdt, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8666714/
https://www.ncbi.nlm.nih.gov/pubmed/34917976
http://dx.doi.org/10.1016/j.xpro.2021.100988
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author Manstein, Felix
Ullmann, Kevin
Triebert, Wiebke
Zweigerdt, Robert
author_facet Manstein, Felix
Ullmann, Kevin
Triebert, Wiebke
Zweigerdt, Robert
author_sort Manstein, Felix
collection PubMed
description The routine therapeutic and industrial applications of human pluripotent stem cells (hPSCs) require their constant mass supply by robust, efficient, and economically viable bioprocesses. Our protocol describes the fully controlled expansion of hPSCs in stirred tank bioreactors (STBRs) enabling cell densities of 35 × 10(6) cells/mL while reducing culture medium consumption by 75%. This is achieved by in silico process modeling and computable upscaling. We provide a detailed blueprint for systematic process development of hPSCs and their progenies. For complete details on the use and execution of this protocol, please refer to Manstein et al. (2021).
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spelling pubmed-86667142021-12-15 Process control and in silico modeling strategies for enabling high density culture of human pluripotent stem cells in stirred tank bioreactors Manstein, Felix Ullmann, Kevin Triebert, Wiebke Zweigerdt, Robert STAR Protoc Protocol The routine therapeutic and industrial applications of human pluripotent stem cells (hPSCs) require their constant mass supply by robust, efficient, and economically viable bioprocesses. Our protocol describes the fully controlled expansion of hPSCs in stirred tank bioreactors (STBRs) enabling cell densities of 35 × 10(6) cells/mL while reducing culture medium consumption by 75%. This is achieved by in silico process modeling and computable upscaling. We provide a detailed blueprint for systematic process development of hPSCs and their progenies. For complete details on the use and execution of this protocol, please refer to Manstein et al. (2021). Elsevier 2021-12-09 /pmc/articles/PMC8666714/ /pubmed/34917976 http://dx.doi.org/10.1016/j.xpro.2021.100988 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Manstein, Felix
Ullmann, Kevin
Triebert, Wiebke
Zweigerdt, Robert
Process control and in silico modeling strategies for enabling high density culture of human pluripotent stem cells in stirred tank bioreactors
title Process control and in silico modeling strategies for enabling high density culture of human pluripotent stem cells in stirred tank bioreactors
title_full Process control and in silico modeling strategies for enabling high density culture of human pluripotent stem cells in stirred tank bioreactors
title_fullStr Process control and in silico modeling strategies for enabling high density culture of human pluripotent stem cells in stirred tank bioreactors
title_full_unstemmed Process control and in silico modeling strategies for enabling high density culture of human pluripotent stem cells in stirred tank bioreactors
title_short Process control and in silico modeling strategies for enabling high density culture of human pluripotent stem cells in stirred tank bioreactors
title_sort process control and in silico modeling strategies for enabling high density culture of human pluripotent stem cells in stirred tank bioreactors
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8666714/
https://www.ncbi.nlm.nih.gov/pubmed/34917976
http://dx.doi.org/10.1016/j.xpro.2021.100988
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