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Design Features to Accelerate the Higher-Order Assembly of DNA Origami on Membranes
[Image: see text] Nanotechnology often exploits DNA origami nanostructures assembled into even larger superstructures up to micrometer sizes with nanometer shape precision. However, large-scale assembly of such structures is very time-consuming. Here, we investigated the efficiency of superstructure...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667037/ https://www.ncbi.nlm.nih.gov/pubmed/34818013 http://dx.doi.org/10.1021/acs.jpcb.1c07694 |
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author | Qutbuddin, Yusuf Krohn, Jan-Hagen Brüggenthies, Gereon A. Stein, Johannes Gavrilovic, Svetozar Stehr, Florian Schwille, Petra |
author_facet | Qutbuddin, Yusuf Krohn, Jan-Hagen Brüggenthies, Gereon A. Stein, Johannes Gavrilovic, Svetozar Stehr, Florian Schwille, Petra |
author_sort | Qutbuddin, Yusuf |
collection | PubMed |
description | [Image: see text] Nanotechnology often exploits DNA origami nanostructures assembled into even larger superstructures up to micrometer sizes with nanometer shape precision. However, large-scale assembly of such structures is very time-consuming. Here, we investigated the efficiency of superstructure assembly on surfaces using indirect cross-linking through low-complexity connector strands binding staple strand extensions, instead of connector strands binding to scaffold loops. Using single-molecule imaging techniques, including fluorescence microscopy and atomic force microscopy, we show that low sequence complexity connector strands allow formation of DNA origami superstructures on lipid membranes, with an order-of-magnitude enhancement in the assembly speed of superstructures. A number of effects, including suppression of DNA hairpin formation, high local effective binding site concentration, and multivalency are proposed to contribute to the acceleration. Thus, the use of low-complexity sequences for DNA origami higher-order assembly offers a very simple but efficient way of improving throughput in DNA origami design. |
format | Online Article Text |
id | pubmed-8667037 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-86670372021-12-14 Design Features to Accelerate the Higher-Order Assembly of DNA Origami on Membranes Qutbuddin, Yusuf Krohn, Jan-Hagen Brüggenthies, Gereon A. Stein, Johannes Gavrilovic, Svetozar Stehr, Florian Schwille, Petra J Phys Chem B [Image: see text] Nanotechnology often exploits DNA origami nanostructures assembled into even larger superstructures up to micrometer sizes with nanometer shape precision. However, large-scale assembly of such structures is very time-consuming. Here, we investigated the efficiency of superstructure assembly on surfaces using indirect cross-linking through low-complexity connector strands binding staple strand extensions, instead of connector strands binding to scaffold loops. Using single-molecule imaging techniques, including fluorescence microscopy and atomic force microscopy, we show that low sequence complexity connector strands allow formation of DNA origami superstructures on lipid membranes, with an order-of-magnitude enhancement in the assembly speed of superstructures. A number of effects, including suppression of DNA hairpin formation, high local effective binding site concentration, and multivalency are proposed to contribute to the acceleration. Thus, the use of low-complexity sequences for DNA origami higher-order assembly offers a very simple but efficient way of improving throughput in DNA origami design. American Chemical Society 2021-11-24 2021-12-09 /pmc/articles/PMC8667037/ /pubmed/34818013 http://dx.doi.org/10.1021/acs.jpcb.1c07694 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Qutbuddin, Yusuf Krohn, Jan-Hagen Brüggenthies, Gereon A. Stein, Johannes Gavrilovic, Svetozar Stehr, Florian Schwille, Petra Design Features to Accelerate the Higher-Order Assembly of DNA Origami on Membranes |
title | Design Features to Accelerate the Higher-Order Assembly
of DNA Origami on Membranes |
title_full | Design Features to Accelerate the Higher-Order Assembly
of DNA Origami on Membranes |
title_fullStr | Design Features to Accelerate the Higher-Order Assembly
of DNA Origami on Membranes |
title_full_unstemmed | Design Features to Accelerate the Higher-Order Assembly
of DNA Origami on Membranes |
title_short | Design Features to Accelerate the Higher-Order Assembly
of DNA Origami on Membranes |
title_sort | design features to accelerate the higher-order assembly
of dna origami on membranes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667037/ https://www.ncbi.nlm.nih.gov/pubmed/34818013 http://dx.doi.org/10.1021/acs.jpcb.1c07694 |
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