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Characterization of microRNAs in spent culture medium associated with human embryo quality and development
BACKGROUND: Given implantation failure limits the improvement of in vitro fertilization (IVF) success rates, there is an urgency to identify potential biomarkers for embryo quality and predict the outcomes of IVF-embryo transfer (IVF-ET). METHODS: Using RNA-sequencing, we identified the expression p...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AME Publishing Company
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667117/ https://www.ncbi.nlm.nih.gov/pubmed/34988157 http://dx.doi.org/10.21037/atm-21-5029 |
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author | Wang, Shanshan Chen, Lei Zhu, Yingchun Jiang, Weihua |
author_facet | Wang, Shanshan Chen, Lei Zhu, Yingchun Jiang, Weihua |
author_sort | Wang, Shanshan |
collection | PubMed |
description | BACKGROUND: Given implantation failure limits the improvement of in vitro fertilization (IVF) success rates, there is an urgency to identify potential biomarkers for embryo quality and predict the outcomes of IVF-embryo transfer (IVF-ET). METHODS: Using RNA-sequencing, we identified the expression profiles of 16 spent culture medium (SCM) collected from embryos at the cleavage on day 3 (D3 cleavage) and blastocyst stages on day 5 (D5 blastocyst) during IVF cycles. Differentially expressed miRNAs (DEmiRNAs) were then identified, and microRNA (miRNA)-messenger RNA (mRNA) interaction networks were constructed. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) confirmation and validation in the Gene Expression Omnibus (GEO) database were performed. RESULTS: Compared with the pregnant group, 29 DEmiRNAs were detected in the non-pregnant group at D3 cleavage, and 26 were detected in the non-pregnant group at D5 blastocyst. Among them, a total of six known miRNAs, including hsa-miR-199a-3p>hsa-miR-199b-3p, hsa-miR-199a-5p, hsa-miR-379-5p, hsa-miR-432-5p, hsa-miR-99a-5p, and hsa-miR-483-5p, were identified. The results of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that these target genes of DEmiRNAs were associated with various biological processes (BPs). The results of validation in qRT-PCR and the GEO database suggested the reliability of our RNA-sequencing results. CONCLUSIONS: In conclusion, we identified three miRNAs, including hsa-miR-199a-5p, hsa-miR-483-5p, and hsa-miR-432-5p, which may serve as biomarkers for embryo quality during IVF cycles. |
format | Online Article Text |
id | pubmed-8667117 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | AME Publishing Company |
record_format | MEDLINE/PubMed |
spelling | pubmed-86671172022-01-04 Characterization of microRNAs in spent culture medium associated with human embryo quality and development Wang, Shanshan Chen, Lei Zhu, Yingchun Jiang, Weihua Ann Transl Med Original Article BACKGROUND: Given implantation failure limits the improvement of in vitro fertilization (IVF) success rates, there is an urgency to identify potential biomarkers for embryo quality and predict the outcomes of IVF-embryo transfer (IVF-ET). METHODS: Using RNA-sequencing, we identified the expression profiles of 16 spent culture medium (SCM) collected from embryos at the cleavage on day 3 (D3 cleavage) and blastocyst stages on day 5 (D5 blastocyst) during IVF cycles. Differentially expressed miRNAs (DEmiRNAs) were then identified, and microRNA (miRNA)-messenger RNA (mRNA) interaction networks were constructed. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) confirmation and validation in the Gene Expression Omnibus (GEO) database were performed. RESULTS: Compared with the pregnant group, 29 DEmiRNAs were detected in the non-pregnant group at D3 cleavage, and 26 were detected in the non-pregnant group at D5 blastocyst. Among them, a total of six known miRNAs, including hsa-miR-199a-3p>hsa-miR-199b-3p, hsa-miR-199a-5p, hsa-miR-379-5p, hsa-miR-432-5p, hsa-miR-99a-5p, and hsa-miR-483-5p, were identified. The results of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that these target genes of DEmiRNAs were associated with various biological processes (BPs). The results of validation in qRT-PCR and the GEO database suggested the reliability of our RNA-sequencing results. CONCLUSIONS: In conclusion, we identified three miRNAs, including hsa-miR-199a-5p, hsa-miR-483-5p, and hsa-miR-432-5p, which may serve as biomarkers for embryo quality during IVF cycles. AME Publishing Company 2021-11 /pmc/articles/PMC8667117/ /pubmed/34988157 http://dx.doi.org/10.21037/atm-21-5029 Text en 2021 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Original Article Wang, Shanshan Chen, Lei Zhu, Yingchun Jiang, Weihua Characterization of microRNAs in spent culture medium associated with human embryo quality and development |
title | Characterization of microRNAs in spent culture medium associated with human embryo quality and development |
title_full | Characterization of microRNAs in spent culture medium associated with human embryo quality and development |
title_fullStr | Characterization of microRNAs in spent culture medium associated with human embryo quality and development |
title_full_unstemmed | Characterization of microRNAs in spent culture medium associated with human embryo quality and development |
title_short | Characterization of microRNAs in spent culture medium associated with human embryo quality and development |
title_sort | characterization of micrornas in spent culture medium associated with human embryo quality and development |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667117/ https://www.ncbi.nlm.nih.gov/pubmed/34988157 http://dx.doi.org/10.21037/atm-21-5029 |
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