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Characterization of microRNAs in spent culture medium associated with human embryo quality and development

BACKGROUND: Given implantation failure limits the improvement of in vitro fertilization (IVF) success rates, there is an urgency to identify potential biomarkers for embryo quality and predict the outcomes of IVF-embryo transfer (IVF-ET). METHODS: Using RNA-sequencing, we identified the expression p...

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Autores principales: Wang, Shanshan, Chen, Lei, Zhu, Yingchun, Jiang, Weihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667117/
https://www.ncbi.nlm.nih.gov/pubmed/34988157
http://dx.doi.org/10.21037/atm-21-5029
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author Wang, Shanshan
Chen, Lei
Zhu, Yingchun
Jiang, Weihua
author_facet Wang, Shanshan
Chen, Lei
Zhu, Yingchun
Jiang, Weihua
author_sort Wang, Shanshan
collection PubMed
description BACKGROUND: Given implantation failure limits the improvement of in vitro fertilization (IVF) success rates, there is an urgency to identify potential biomarkers for embryo quality and predict the outcomes of IVF-embryo transfer (IVF-ET). METHODS: Using RNA-sequencing, we identified the expression profiles of 16 spent culture medium (SCM) collected from embryos at the cleavage on day 3 (D3 cleavage) and blastocyst stages on day 5 (D5 blastocyst) during IVF cycles. Differentially expressed miRNAs (DEmiRNAs) were then identified, and microRNA (miRNA)-messenger RNA (mRNA) interaction networks were constructed. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) confirmation and validation in the Gene Expression Omnibus (GEO) database were performed. RESULTS: Compared with the pregnant group, 29 DEmiRNAs were detected in the non-pregnant group at D3 cleavage, and 26 were detected in the non-pregnant group at D5 blastocyst. Among them, a total of six known miRNAs, including hsa-miR-199a-3p>hsa-miR-199b-3p, hsa-miR-199a-5p, hsa-miR-379-5p, hsa-miR-432-5p, hsa-miR-99a-5p, and hsa-miR-483-5p, were identified. The results of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that these target genes of DEmiRNAs were associated with various biological processes (BPs). The results of validation in qRT-PCR and the GEO database suggested the reliability of our RNA-sequencing results. CONCLUSIONS: In conclusion, we identified three miRNAs, including hsa-miR-199a-5p, hsa-miR-483-5p, and hsa-miR-432-5p, which may serve as biomarkers for embryo quality during IVF cycles.
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spelling pubmed-86671172022-01-04 Characterization of microRNAs in spent culture medium associated with human embryo quality and development Wang, Shanshan Chen, Lei Zhu, Yingchun Jiang, Weihua Ann Transl Med Original Article BACKGROUND: Given implantation failure limits the improvement of in vitro fertilization (IVF) success rates, there is an urgency to identify potential biomarkers for embryo quality and predict the outcomes of IVF-embryo transfer (IVF-ET). METHODS: Using RNA-sequencing, we identified the expression profiles of 16 spent culture medium (SCM) collected from embryos at the cleavage on day 3 (D3 cleavage) and blastocyst stages on day 5 (D5 blastocyst) during IVF cycles. Differentially expressed miRNAs (DEmiRNAs) were then identified, and microRNA (miRNA)-messenger RNA (mRNA) interaction networks were constructed. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) confirmation and validation in the Gene Expression Omnibus (GEO) database were performed. RESULTS: Compared with the pregnant group, 29 DEmiRNAs were detected in the non-pregnant group at D3 cleavage, and 26 were detected in the non-pregnant group at D5 blastocyst. Among them, a total of six known miRNAs, including hsa-miR-199a-3p>hsa-miR-199b-3p, hsa-miR-199a-5p, hsa-miR-379-5p, hsa-miR-432-5p, hsa-miR-99a-5p, and hsa-miR-483-5p, were identified. The results of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that these target genes of DEmiRNAs were associated with various biological processes (BPs). The results of validation in qRT-PCR and the GEO database suggested the reliability of our RNA-sequencing results. CONCLUSIONS: In conclusion, we identified three miRNAs, including hsa-miR-199a-5p, hsa-miR-483-5p, and hsa-miR-432-5p, which may serve as biomarkers for embryo quality during IVF cycles. AME Publishing Company 2021-11 /pmc/articles/PMC8667117/ /pubmed/34988157 http://dx.doi.org/10.21037/atm-21-5029 Text en 2021 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Wang, Shanshan
Chen, Lei
Zhu, Yingchun
Jiang, Weihua
Characterization of microRNAs in spent culture medium associated with human embryo quality and development
title Characterization of microRNAs in spent culture medium associated with human embryo quality and development
title_full Characterization of microRNAs in spent culture medium associated with human embryo quality and development
title_fullStr Characterization of microRNAs in spent culture medium associated with human embryo quality and development
title_full_unstemmed Characterization of microRNAs in spent culture medium associated with human embryo quality and development
title_short Characterization of microRNAs in spent culture medium associated with human embryo quality and development
title_sort characterization of micrornas in spent culture medium associated with human embryo quality and development
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667117/
https://www.ncbi.nlm.nih.gov/pubmed/34988157
http://dx.doi.org/10.21037/atm-21-5029
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