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Effect of cigarette smoke extract on mitochondrial division in mouse quadriceps femoris cells

BACKGROUND: To observe the effect of cigarette smoke extract (CSE) on mitochondrial division in mouse quadriceps femoris cells and to explore the potential molecular mechanism of skeletal muscle dysfunction (SMD) in patients with chronic obstructive pulmonary disease (COPD). METHODS: Quadriceps femo...

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Autores principales: Tan, Zhidan, Li, Siqi, Zhu, Su, Yao, Xiaoxuan, Li, Jing, Gao, Xinglin, Yang, Shifang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667143/
https://www.ncbi.nlm.nih.gov/pubmed/34988208
http://dx.doi.org/10.21037/atm-21-5891
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author Tan, Zhidan
Li, Siqi
Zhu, Su
Yao, Xiaoxuan
Li, Jing
Gao, Xinglin
Yang, Shifang
author_facet Tan, Zhidan
Li, Siqi
Zhu, Su
Yao, Xiaoxuan
Li, Jing
Gao, Xinglin
Yang, Shifang
author_sort Tan, Zhidan
collection PubMed
description BACKGROUND: To observe the effect of cigarette smoke extract (CSE) on mitochondrial division in mouse quadriceps femoris cells and to explore the potential molecular mechanism of skeletal muscle dysfunction (SMD) in patients with chronic obstructive pulmonary disease (COPD). METHODS: Quadriceps femoris were cultured, passaged, and stimulated with different concentrations of CSE. We divided cells into four groups (Control, 2.5%, 5%, 10%). The growth of cells, the expression of Dynamin related protein 1 (Drp-1), and apoptosis were observed and evaluated by fluorescence microscopy, RT-PCR, Western blot, and flow cytometry. RESULTS: The longer the intervention time, the more obvious the decrease in cell number. In the 5% and 10% groups, the cells became round with gaps. Under an inverted fluorescence microscope, the green fluorescence of cells in 5% and 10% stained with Mito-Tracker Green was significantly less than that of the Control and 2.5%. Red fluorescence was reduced and the green fluorescence was increased in the 5% and 10% stained with JC-1. Flow cytometry analysis showed that reactive oxygen species (ROS) and apoptosis were increased in the CSE intervention groups. In the Control, 2.5%, 5%, and 10%, the levels of ROS were 0.052±0.015, 0.170±0.030, 5.340±0.500, and 24.400±1.900, respectively. The apoptotic rates (%) were 0.270±0.009, 2.650±0.060, 11.850±0.020, and 31.820±1.260, respectively. The relative expression levels were, 0.900±0.093, 1.141±0.099, 1.361±0.034, 2.155±0.092 for DNM1L mRNA, and 0.509±0.008, 0.569±0.028, 0.792±0.048, 0.940±0.062 for Drp-1. There were significant differences in the apoptotic rate, and Drp-1 expression between 5% and 10% compared with the Control and 2.5% (P<0.05). CONCLUSIONS: CSE may enhance mitochondrial division of quadriceps femoris cells by up-regulating the expression of Drp-1, affecting cellular energy metabolism and promoting quadriceps femoris apoptosis, ultimately leading to the occurrence and development of skeletal muscle dysfunction in COPD.
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spelling pubmed-86671432022-01-04 Effect of cigarette smoke extract on mitochondrial division in mouse quadriceps femoris cells Tan, Zhidan Li, Siqi Zhu, Su Yao, Xiaoxuan Li, Jing Gao, Xinglin Yang, Shifang Ann Transl Med Original Article BACKGROUND: To observe the effect of cigarette smoke extract (CSE) on mitochondrial division in mouse quadriceps femoris cells and to explore the potential molecular mechanism of skeletal muscle dysfunction (SMD) in patients with chronic obstructive pulmonary disease (COPD). METHODS: Quadriceps femoris were cultured, passaged, and stimulated with different concentrations of CSE. We divided cells into four groups (Control, 2.5%, 5%, 10%). The growth of cells, the expression of Dynamin related protein 1 (Drp-1), and apoptosis were observed and evaluated by fluorescence microscopy, RT-PCR, Western blot, and flow cytometry. RESULTS: The longer the intervention time, the more obvious the decrease in cell number. In the 5% and 10% groups, the cells became round with gaps. Under an inverted fluorescence microscope, the green fluorescence of cells in 5% and 10% stained with Mito-Tracker Green was significantly less than that of the Control and 2.5%. Red fluorescence was reduced and the green fluorescence was increased in the 5% and 10% stained with JC-1. Flow cytometry analysis showed that reactive oxygen species (ROS) and apoptosis were increased in the CSE intervention groups. In the Control, 2.5%, 5%, and 10%, the levels of ROS were 0.052±0.015, 0.170±0.030, 5.340±0.500, and 24.400±1.900, respectively. The apoptotic rates (%) were 0.270±0.009, 2.650±0.060, 11.850±0.020, and 31.820±1.260, respectively. The relative expression levels were, 0.900±0.093, 1.141±0.099, 1.361±0.034, 2.155±0.092 for DNM1L mRNA, and 0.509±0.008, 0.569±0.028, 0.792±0.048, 0.940±0.062 for Drp-1. There were significant differences in the apoptotic rate, and Drp-1 expression between 5% and 10% compared with the Control and 2.5% (P<0.05). CONCLUSIONS: CSE may enhance mitochondrial division of quadriceps femoris cells by up-regulating the expression of Drp-1, affecting cellular energy metabolism and promoting quadriceps femoris apoptosis, ultimately leading to the occurrence and development of skeletal muscle dysfunction in COPD. AME Publishing Company 2021-11 /pmc/articles/PMC8667143/ /pubmed/34988208 http://dx.doi.org/10.21037/atm-21-5891 Text en 2021 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Tan, Zhidan
Li, Siqi
Zhu, Su
Yao, Xiaoxuan
Li, Jing
Gao, Xinglin
Yang, Shifang
Effect of cigarette smoke extract on mitochondrial division in mouse quadriceps femoris cells
title Effect of cigarette smoke extract on mitochondrial division in mouse quadriceps femoris cells
title_full Effect of cigarette smoke extract on mitochondrial division in mouse quadriceps femoris cells
title_fullStr Effect of cigarette smoke extract on mitochondrial division in mouse quadriceps femoris cells
title_full_unstemmed Effect of cigarette smoke extract on mitochondrial division in mouse quadriceps femoris cells
title_short Effect of cigarette smoke extract on mitochondrial division in mouse quadriceps femoris cells
title_sort effect of cigarette smoke extract on mitochondrial division in mouse quadriceps femoris cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667143/
https://www.ncbi.nlm.nih.gov/pubmed/34988208
http://dx.doi.org/10.21037/atm-21-5891
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