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CircularLRRC7 is a Potential Tumor Suppressor Associated With miR-1281 and PDXP Expression in Glioblastoma

Circular RNAs (circRNAs) are usually enriched in neural tissues, yet about 80% circRNAs have lower expression in gliomas relative to normal brains, highlighting the importance of circRNAs as tumor suppressors. However, the clinical impact as well as the pathways regulated by the tumor-suppressive ci...

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Autores principales: Kong, Xue, Xu, Ruiting, Wang, Wei, Zeng, Minghui, Li, Yuan, Lin, Mengyu, Zhou, Wenchao, Fu, Xianming, Wu, Haibo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667166/
https://www.ncbi.nlm.nih.gov/pubmed/34912844
http://dx.doi.org/10.3389/fmolb.2021.743417
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author Kong, Xue
Xu, Ruiting
Wang, Wei
Zeng, Minghui
Li, Yuan
Lin, Mengyu
Zhou, Wenchao
Fu, Xianming
Wu, Haibo
author_facet Kong, Xue
Xu, Ruiting
Wang, Wei
Zeng, Minghui
Li, Yuan
Lin, Mengyu
Zhou, Wenchao
Fu, Xianming
Wu, Haibo
author_sort Kong, Xue
collection PubMed
description Circular RNAs (circRNAs) are usually enriched in neural tissues, yet about 80% circRNAs have lower expression in gliomas relative to normal brains, highlighting the importance of circRNAs as tumor suppressors. However, the clinical impact as well as the pathways regulated by the tumor-suppressive circRNAs remain largely unknown in glioblastoma (GBM). Through bioinformatic analysis followed by experimental validation, we found that hsa_circ_0114014 (circLRRC7) was dramatically down-regulated in GBM when compared with normal brain tissues (p < 0.0001). GBM patients with a lower circLRRC7 expression had poorer progression-free survival (PFS, p < 0.05) and overall survival (OS, p < 0.05). Analyses of the predicted target miRNAs of circLRRC7 in CSCD and CRI databases, in combination with the miRNA expression data in GBMs and normal brains from GSE database, revealed miR-1281 as a potential downstream target of circLRRC7. Subsequently, the target genes of hsa-mir-1281 were predicted by TargetScan, miRDB and miRNATAR databases. Intersection analysis and correlation test indicated that PDXP was a potential target of miR-1281. In summary, circLRRC7 may be a tumor suppressor that associated with miR-1281 and PDXP expression in GBM, which may provide novel therapeutic targets for GBM treatment.
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spelling pubmed-86671662021-12-14 CircularLRRC7 is a Potential Tumor Suppressor Associated With miR-1281 and PDXP Expression in Glioblastoma Kong, Xue Xu, Ruiting Wang, Wei Zeng, Minghui Li, Yuan Lin, Mengyu Zhou, Wenchao Fu, Xianming Wu, Haibo Front Mol Biosci Molecular Biosciences Circular RNAs (circRNAs) are usually enriched in neural tissues, yet about 80% circRNAs have lower expression in gliomas relative to normal brains, highlighting the importance of circRNAs as tumor suppressors. However, the clinical impact as well as the pathways regulated by the tumor-suppressive circRNAs remain largely unknown in glioblastoma (GBM). Through bioinformatic analysis followed by experimental validation, we found that hsa_circ_0114014 (circLRRC7) was dramatically down-regulated in GBM when compared with normal brain tissues (p < 0.0001). GBM patients with a lower circLRRC7 expression had poorer progression-free survival (PFS, p < 0.05) and overall survival (OS, p < 0.05). Analyses of the predicted target miRNAs of circLRRC7 in CSCD and CRI databases, in combination with the miRNA expression data in GBMs and normal brains from GSE database, revealed miR-1281 as a potential downstream target of circLRRC7. Subsequently, the target genes of hsa-mir-1281 were predicted by TargetScan, miRDB and miRNATAR databases. Intersection analysis and correlation test indicated that PDXP was a potential target of miR-1281. In summary, circLRRC7 may be a tumor suppressor that associated with miR-1281 and PDXP expression in GBM, which may provide novel therapeutic targets for GBM treatment. Frontiers Media S.A. 2021-11-29 /pmc/articles/PMC8667166/ /pubmed/34912844 http://dx.doi.org/10.3389/fmolb.2021.743417 Text en Copyright © 2021 Kong, Xu, Wang, Zeng, Li, Lin, Zhou, Fu and Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Kong, Xue
Xu, Ruiting
Wang, Wei
Zeng, Minghui
Li, Yuan
Lin, Mengyu
Zhou, Wenchao
Fu, Xianming
Wu, Haibo
CircularLRRC7 is a Potential Tumor Suppressor Associated With miR-1281 and PDXP Expression in Glioblastoma
title CircularLRRC7 is a Potential Tumor Suppressor Associated With miR-1281 and PDXP Expression in Glioblastoma
title_full CircularLRRC7 is a Potential Tumor Suppressor Associated With miR-1281 and PDXP Expression in Glioblastoma
title_fullStr CircularLRRC7 is a Potential Tumor Suppressor Associated With miR-1281 and PDXP Expression in Glioblastoma
title_full_unstemmed CircularLRRC7 is a Potential Tumor Suppressor Associated With miR-1281 and PDXP Expression in Glioblastoma
title_short CircularLRRC7 is a Potential Tumor Suppressor Associated With miR-1281 and PDXP Expression in Glioblastoma
title_sort circularlrrc7 is a potential tumor suppressor associated with mir-1281 and pdxp expression in glioblastoma
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667166/
https://www.ncbi.nlm.nih.gov/pubmed/34912844
http://dx.doi.org/10.3389/fmolb.2021.743417
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