Standardization of two SARS-CoV-2 serology assays to the WHO 20/136 human standard reference material

WHO 20/136 is standard reference material for SARS−COV-2 serology assays. Standardization of serology assays that target the same antigen and class of immunoglobulin will enable comparison of results between studies that use various lab-developed and commercial assays around the world. Standardizati...

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Detalles Bibliográficos
Autores principales: Freeman, James, Conklin, Justin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667347/
https://www.ncbi.nlm.nih.gov/pubmed/34915088
http://dx.doi.org/10.1016/j.jviromet.2021.114430
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author Freeman, James
Conklin, Justin
author_facet Freeman, James
Conklin, Justin
author_sort Freeman, James
collection PubMed
description WHO 20/136 is standard reference material for SARS−COV-2 serology assays. Standardization of serology assays that target the same antigen and class of immunoglobulin will enable comparison of results between studies that use various lab-developed and commercial assays around the world. Standardization of assays will help better define immune correlates of protection and possibly immune correlates of vaccine efficacy. Two automated SARS−COV-2 anti-S1 RBD immunoglobulin serology assays on the Atellica IM Analyzer were calibrated to WHO 20/136 Standard Reference Material which was assigned 1000 binding antibody units (BAU/mL). The anti-S1 RBD IgG assay (sCOVG) cut-off Index of 1.00 corresponded to WHO 45.1 BAU/mL, and the anti-S1 RBD Ig Total assay (COV2T) cut-off Index of 1.00 corresponded to WHO 6.70 BAU/mL.
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spelling pubmed-86673472021-12-14 Standardization of two SARS-CoV-2 serology assays to the WHO 20/136 human standard reference material Freeman, James Conklin, Justin J Virol Methods Short Communication WHO 20/136 is standard reference material for SARS−COV-2 serology assays. Standardization of serology assays that target the same antigen and class of immunoglobulin will enable comparison of results between studies that use various lab-developed and commercial assays around the world. Standardization of assays will help better define immune correlates of protection and possibly immune correlates of vaccine efficacy. Two automated SARS−COV-2 anti-S1 RBD immunoglobulin serology assays on the Atellica IM Analyzer were calibrated to WHO 20/136 Standard Reference Material which was assigned 1000 binding antibody units (BAU/mL). The anti-S1 RBD IgG assay (sCOVG) cut-off Index of 1.00 corresponded to WHO 45.1 BAU/mL, and the anti-S1 RBD Ig Total assay (COV2T) cut-off Index of 1.00 corresponded to WHO 6.70 BAU/mL. Elsevier B.V. 2022-02 2021-12-13 /pmc/articles/PMC8667347/ /pubmed/34915088 http://dx.doi.org/10.1016/j.jviromet.2021.114430 Text en © 2021 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Short Communication
Freeman, James
Conklin, Justin
Standardization of two SARS-CoV-2 serology assays to the WHO 20/136 human standard reference material
title Standardization of two SARS-CoV-2 serology assays to the WHO 20/136 human standard reference material
title_full Standardization of two SARS-CoV-2 serology assays to the WHO 20/136 human standard reference material
title_fullStr Standardization of two SARS-CoV-2 serology assays to the WHO 20/136 human standard reference material
title_full_unstemmed Standardization of two SARS-CoV-2 serology assays to the WHO 20/136 human standard reference material
title_short Standardization of two SARS-CoV-2 serology assays to the WHO 20/136 human standard reference material
title_sort standardization of two sars-cov-2 serology assays to the who 20/136 human standard reference material
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667347/
https://www.ncbi.nlm.nih.gov/pubmed/34915088
http://dx.doi.org/10.1016/j.jviromet.2021.114430
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