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An Exploration of Non-Coding RNAs in Extracellular Vesicles Delivered by Swine Anterior Pituitary
Extracellular vesicles are lipid bilayer-delimited particles carrying proteins, lipids, and small RNAs. Previous studies have demonstrated that they had regulatory functions both physiologically and pathologically. However, information remains inadequate on extracellular vesicles from the anterior p...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667663/ https://www.ncbi.nlm.nih.gov/pubmed/34912377 http://dx.doi.org/10.3389/fgene.2021.772753 |
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author | Xiong, Jiali Zhang, Haojie Zeng, Bin Liu, Jie Luo, Junyi Chen, Ting Sun, Jiajie Xi, Qianyun Zhang, Yongliang |
author_facet | Xiong, Jiali Zhang, Haojie Zeng, Bin Liu, Jie Luo, Junyi Chen, Ting Sun, Jiajie Xi, Qianyun Zhang, Yongliang |
author_sort | Xiong, Jiali |
collection | PubMed |
description | Extracellular vesicles are lipid bilayer-delimited particles carrying proteins, lipids, and small RNAs. Previous studies have demonstrated that they had regulatory functions both physiologically and pathologically. However, information remains inadequate on extracellular vesicles from the anterior pituitary, a key endocrine organ in animals and humans. In this study, we separated and identified extracellular vesicles from the anterior pituitary of the Duroc swine model. Total RNA was extracted and RNA-seq was performed, followed by a comprehensive analysis of miRNAs, lncRNAs, and circRNAs. Resultantly, we obtained 416 miRNAs, 16,232 lncRNAs, and 495 circRNAs. Furthermore, GO and KEGG enrichment analysis showed that the ncRNAs in extracellular vesicles may participate in regulating intracellular signal transduction, cellular component organization or biogenesis, small molecule binding, and transferase activity. The cross-talk between them also suggested that they may play an important role in the signaling process and biological regulation. This is the first report of ncRNA data in the anterior pituitary extracellular vesicles from the duroc swine breed, which is a fundamental resource for exploring detailed functions of extracellular vesicles from the anterior pituitary. |
format | Online Article Text |
id | pubmed-8667663 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86676632021-12-14 An Exploration of Non-Coding RNAs in Extracellular Vesicles Delivered by Swine Anterior Pituitary Xiong, Jiali Zhang, Haojie Zeng, Bin Liu, Jie Luo, Junyi Chen, Ting Sun, Jiajie Xi, Qianyun Zhang, Yongliang Front Genet Genetics Extracellular vesicles are lipid bilayer-delimited particles carrying proteins, lipids, and small RNAs. Previous studies have demonstrated that they had regulatory functions both physiologically and pathologically. However, information remains inadequate on extracellular vesicles from the anterior pituitary, a key endocrine organ in animals and humans. In this study, we separated and identified extracellular vesicles from the anterior pituitary of the Duroc swine model. Total RNA was extracted and RNA-seq was performed, followed by a comprehensive analysis of miRNAs, lncRNAs, and circRNAs. Resultantly, we obtained 416 miRNAs, 16,232 lncRNAs, and 495 circRNAs. Furthermore, GO and KEGG enrichment analysis showed that the ncRNAs in extracellular vesicles may participate in regulating intracellular signal transduction, cellular component organization or biogenesis, small molecule binding, and transferase activity. The cross-talk between them also suggested that they may play an important role in the signaling process and biological regulation. This is the first report of ncRNA data in the anterior pituitary extracellular vesicles from the duroc swine breed, which is a fundamental resource for exploring detailed functions of extracellular vesicles from the anterior pituitary. Frontiers Media S.A. 2021-11-29 /pmc/articles/PMC8667663/ /pubmed/34912377 http://dx.doi.org/10.3389/fgene.2021.772753 Text en Copyright © 2021 Xiong, Zhang, Zeng, Liu, Luo, Chen, Sun, Xi and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Xiong, Jiali Zhang, Haojie Zeng, Bin Liu, Jie Luo, Junyi Chen, Ting Sun, Jiajie Xi, Qianyun Zhang, Yongliang An Exploration of Non-Coding RNAs in Extracellular Vesicles Delivered by Swine Anterior Pituitary |
title | An Exploration of Non-Coding RNAs in Extracellular Vesicles Delivered by Swine Anterior Pituitary |
title_full | An Exploration of Non-Coding RNAs in Extracellular Vesicles Delivered by Swine Anterior Pituitary |
title_fullStr | An Exploration of Non-Coding RNAs in Extracellular Vesicles Delivered by Swine Anterior Pituitary |
title_full_unstemmed | An Exploration of Non-Coding RNAs in Extracellular Vesicles Delivered by Swine Anterior Pituitary |
title_short | An Exploration of Non-Coding RNAs in Extracellular Vesicles Delivered by Swine Anterior Pituitary |
title_sort | exploration of non-coding rnas in extracellular vesicles delivered by swine anterior pituitary |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667663/ https://www.ncbi.nlm.nih.gov/pubmed/34912377 http://dx.doi.org/10.3389/fgene.2021.772753 |
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