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Molecular Characterization and Clinical Relevance of ANXA1 in Gliomas via 1,018 Chinese Cohort Patients

Annexin A1 (ANXA1) is a calcium-dependent phospholipid-binding protein and has been implicated in multiple functions essential in cancer, including cell proliferation, apoptosis, chemosensitivity, metastasis, and invasion. However, the biological role and clinical behavior of ANXA1 in glioma remain...

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Autores principales: Qian, Zenghui, Fan, Wenhua, Meng, Fanlin, Sun, Zhiyan, Li, Guanzhang, Zhai, You, Chang, Yuanhao, Yang, Changlin, Zeng, Fan, Chai, Ruichao, Wu, Fan, Zhao, Zheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667664/
https://www.ncbi.nlm.nih.gov/pubmed/34912807
http://dx.doi.org/10.3389/fcell.2021.777182
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author Qian, Zenghui
Fan, Wenhua
Meng, Fanlin
Sun, Zhiyan
Li, Guanzhang
Zhai, You
Chang, Yuanhao
Yang, Changlin
Zeng, Fan
Chai, Ruichao
Wu, Fan
Zhao, Zheng
author_facet Qian, Zenghui
Fan, Wenhua
Meng, Fanlin
Sun, Zhiyan
Li, Guanzhang
Zhai, You
Chang, Yuanhao
Yang, Changlin
Zeng, Fan
Chai, Ruichao
Wu, Fan
Zhao, Zheng
author_sort Qian, Zenghui
collection PubMed
description Annexin A1 (ANXA1) is a calcium-dependent phospholipid-binding protein and has been implicated in multiple functions essential in cancer, including cell proliferation, apoptosis, chemosensitivity, metastasis, and invasion. However, the biological role and clinical behavior of ANXA1 in glioma remain unclear. In this study, RNA-seq (n = 1018 cases) and whole-exome sequencing (WES) (n = 286 cases) data on a Chinese cohort, RNA-seq data with different histological regions of glioblastoma blocks (n = 270 cases), and scRNA-seq data (n = 7630 cells) were used. We used the R software to perform statistical calculations and graph rendering. We found that ANXA1 is closely related to the malignant progression in gliomas. Meanwhile, ANXA1 is significantly associated with clinical behavior. Furthermore, the mutational profile revealed that glioma subtypes classified by ANXA1 expression showed distinct genetic features. Functional analyses suggest that ANXA1 correlates with the immune-related function and cancer hallmark. At a single-cell level, we found that ANXA1 is highly expressed in M2 macrophages and tumor cells of the mesenchymal subtype. Importantly, our result suggested that ANXA1 expression is significant with the patient’s survival outcome. Our study revealed that ANXA1 was closely related to immune response. ANXA1 plays a key factor in M2 macrophages and MES tumor cells. Patients with lower ANXA1 expression levels tended to experience improved survival. ANXA1 may become a valuable factor for the diagnosis and treatment of gliomas in clinical practice.
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spelling pubmed-86676642021-12-14 Molecular Characterization and Clinical Relevance of ANXA1 in Gliomas via 1,018 Chinese Cohort Patients Qian, Zenghui Fan, Wenhua Meng, Fanlin Sun, Zhiyan Li, Guanzhang Zhai, You Chang, Yuanhao Yang, Changlin Zeng, Fan Chai, Ruichao Wu, Fan Zhao, Zheng Front Cell Dev Biol Cell and Developmental Biology Annexin A1 (ANXA1) is a calcium-dependent phospholipid-binding protein and has been implicated in multiple functions essential in cancer, including cell proliferation, apoptosis, chemosensitivity, metastasis, and invasion. However, the biological role and clinical behavior of ANXA1 in glioma remain unclear. In this study, RNA-seq (n = 1018 cases) and whole-exome sequencing (WES) (n = 286 cases) data on a Chinese cohort, RNA-seq data with different histological regions of glioblastoma blocks (n = 270 cases), and scRNA-seq data (n = 7630 cells) were used. We used the R software to perform statistical calculations and graph rendering. We found that ANXA1 is closely related to the malignant progression in gliomas. Meanwhile, ANXA1 is significantly associated with clinical behavior. Furthermore, the mutational profile revealed that glioma subtypes classified by ANXA1 expression showed distinct genetic features. Functional analyses suggest that ANXA1 correlates with the immune-related function and cancer hallmark. At a single-cell level, we found that ANXA1 is highly expressed in M2 macrophages and tumor cells of the mesenchymal subtype. Importantly, our result suggested that ANXA1 expression is significant with the patient’s survival outcome. Our study revealed that ANXA1 was closely related to immune response. ANXA1 plays a key factor in M2 macrophages and MES tumor cells. Patients with lower ANXA1 expression levels tended to experience improved survival. ANXA1 may become a valuable factor for the diagnosis and treatment of gliomas in clinical practice. Frontiers Media S.A. 2021-11-29 /pmc/articles/PMC8667664/ /pubmed/34912807 http://dx.doi.org/10.3389/fcell.2021.777182 Text en Copyright © 2021 Qian, Fan, Meng, Sun, Li, Zhai, Chang, Yang, Zeng, Chai, Wu and Zhao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Qian, Zenghui
Fan, Wenhua
Meng, Fanlin
Sun, Zhiyan
Li, Guanzhang
Zhai, You
Chang, Yuanhao
Yang, Changlin
Zeng, Fan
Chai, Ruichao
Wu, Fan
Zhao, Zheng
Molecular Characterization and Clinical Relevance of ANXA1 in Gliomas via 1,018 Chinese Cohort Patients
title Molecular Characterization and Clinical Relevance of ANXA1 in Gliomas via 1,018 Chinese Cohort Patients
title_full Molecular Characterization and Clinical Relevance of ANXA1 in Gliomas via 1,018 Chinese Cohort Patients
title_fullStr Molecular Characterization and Clinical Relevance of ANXA1 in Gliomas via 1,018 Chinese Cohort Patients
title_full_unstemmed Molecular Characterization and Clinical Relevance of ANXA1 in Gliomas via 1,018 Chinese Cohort Patients
title_short Molecular Characterization and Clinical Relevance of ANXA1 in Gliomas via 1,018 Chinese Cohort Patients
title_sort molecular characterization and clinical relevance of anxa1 in gliomas via 1,018 chinese cohort patients
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667664/
https://www.ncbi.nlm.nih.gov/pubmed/34912807
http://dx.doi.org/10.3389/fcell.2021.777182
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