Identification of Theileria spp. in sheep and goats from Jeddah, Saudi Arabia, using molecular techniques

BACKGROUND: Thileriosis is a tick -born disease caused by hemoprotozoan parasites which has global veterinary and economic implications. METHODS: Blood samples were collected from 216 sheep and 83 goats from Jeddah, Saudi Arabia, were analyzed to determine whether the animals were infected with Thei...

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Autores principales: Metwally, Dina M., Alajmi, Reem, Alsulami, Muslimah N., Al-Turaiki, Isra M., Abdel-Gaber, Rewaida, Alkhuriji, Afrah F., Albohiri, Haleema H., Mohamed, Khalil, Baghdadi, Hanadi B., El-Khadragy, Manal F., Isaias, Guillermo T., El-Ashram, Saeed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Bioinformatics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667737/
https://www.ncbi.nlm.nih.gov/pubmed/34966592
http://dx.doi.org/10.7717/peerj.12596
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author Metwally, Dina M.
Alajmi, Reem
Alsulami, Muslimah N.
Al-Turaiki, Isra M.
Abdel-Gaber, Rewaida
Alkhuriji, Afrah F.
Albohiri, Haleema H.
Mohamed, Khalil
Baghdadi, Hanadi B.
El-Khadragy, Manal F.
Isaias, Guillermo T.
El-Ashram, Saeed
author_facet Metwally, Dina M.
Alajmi, Reem
Alsulami, Muslimah N.
Al-Turaiki, Isra M.
Abdel-Gaber, Rewaida
Alkhuriji, Afrah F.
Albohiri, Haleema H.
Mohamed, Khalil
Baghdadi, Hanadi B.
El-Khadragy, Manal F.
Isaias, Guillermo T.
El-Ashram, Saeed
author_sort Metwally, Dina M.
collection PubMed
description BACKGROUND: Thileriosis is a tick -born disease caused by hemoprotozoan parasites which has global veterinary and economic implications. METHODS: Blood samples were collected from 216 sheep and 83 goats from Jeddah, Saudi Arabia, were analyzed to determine whether the animals were infected with Theileria spp. parasites. The parasites were detected using a polymerase chain reaction (PCR) targeting the gene of 18S rRNA followed by sequencing. RESULTS: According to obtained findings, Theileria spp. were detected in sheep (57.8%, 48/83) and goats (51.9%, 112/216). Phylogenetic analysis to sequence data showed that T. ovis identified in this study were found to be closely connected to an isolate from Turkey, with 84.4–99.8% pairwise identity and 52.35–99.79% coverage.
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spelling pubmed-86677372021-12-28 Identification of Theileria spp. in sheep and goats from Jeddah, Saudi Arabia, using molecular techniques Metwally, Dina M. Alajmi, Reem Alsulami, Muslimah N. Al-Turaiki, Isra M. Abdel-Gaber, Rewaida Alkhuriji, Afrah F. Albohiri, Haleema H. Mohamed, Khalil Baghdadi, Hanadi B. El-Khadragy, Manal F. Isaias, Guillermo T. El-Ashram, Saeed PeerJ Bioinformatics BACKGROUND: Thileriosis is a tick -born disease caused by hemoprotozoan parasites which has global veterinary and economic implications. METHODS: Blood samples were collected from 216 sheep and 83 goats from Jeddah, Saudi Arabia, were analyzed to determine whether the animals were infected with Theileria spp. parasites. The parasites were detected using a polymerase chain reaction (PCR) targeting the gene of 18S rRNA followed by sequencing. RESULTS: According to obtained findings, Theileria spp. were detected in sheep (57.8%, 48/83) and goats (51.9%, 112/216). Phylogenetic analysis to sequence data showed that T. ovis identified in this study were found to be closely connected to an isolate from Turkey, with 84.4–99.8% pairwise identity and 52.35–99.79% coverage. PeerJ Inc. 2021-12-10 /pmc/articles/PMC8667737/ /pubmed/34966592 http://dx.doi.org/10.7717/peerj.12596 Text en ©2021 Metwally et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Bioinformatics
Metwally, Dina M.
Alajmi, Reem
Alsulami, Muslimah N.
Al-Turaiki, Isra M.
Abdel-Gaber, Rewaida
Alkhuriji, Afrah F.
Albohiri, Haleema H.
Mohamed, Khalil
Baghdadi, Hanadi B.
El-Khadragy, Manal F.
Isaias, Guillermo T.
El-Ashram, Saeed
Identification of Theileria spp. in sheep and goats from Jeddah, Saudi Arabia, using molecular techniques
title Identification of Theileria spp. in sheep and goats from Jeddah, Saudi Arabia, using molecular techniques
title_full Identification of Theileria spp. in sheep and goats from Jeddah, Saudi Arabia, using molecular techniques
title_fullStr Identification of Theileria spp. in sheep and goats from Jeddah, Saudi Arabia, using molecular techniques
title_full_unstemmed Identification of Theileria spp. in sheep and goats from Jeddah, Saudi Arabia, using molecular techniques
title_short Identification of Theileria spp. in sheep and goats from Jeddah, Saudi Arabia, using molecular techniques
title_sort identification of theileria spp. in sheep and goats from jeddah, saudi arabia, using molecular techniques
topic Bioinformatics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667737/
https://www.ncbi.nlm.nih.gov/pubmed/34966592
http://dx.doi.org/10.7717/peerj.12596
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