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Cy3-ATP labeling of unfixed, permeabilized mouse hair cells

ATP-utilizing enzymes play key roles in hair bundles, the mechanically sensitive organelles of sensory hair cells in the inner ear. We used a fluorescent ATP analog, EDA-ATP-Cy3 (Cy3-ATP), to label ATP-binding proteins in two different preparations of unfixed hair-cell stereocilia of the mouse. In t...

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Detalles Bibliográficos
Autores principales: Pacentine, Itallia V., Barr-Gillespie, Peter G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8668996/
https://www.ncbi.nlm.nih.gov/pubmed/34903829
http://dx.doi.org/10.1038/s41598-021-03365-x

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