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Generation of mutant pigs by lipofection-mediated genome editing in embryos

The specificity and efficiency of CRISPR/Cas9 gene-editing systems are determined by several factors, including the mode of delivery, when applied to mammalian embryos. Given the limited time window for delivery, faster and more reliable methods to introduce Cas9-gRNA ribonucleoprotein complexes (RN...

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Detalles Bibliográficos
Autores principales:	Hirata, Maki, Wittayarat, Manita, Namula, Zhao, Le, Quynh Anh, Lin, Qingyi, Takebayashi, Koki, Thongkittidilok, Chommanart, Mito, Taro, Tomonari, Sayuri, Tanihara, Fuminori, Otoi, Takeshige
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8668999/ https://www.ncbi.nlm.nih.gov/pubmed/34903813 http://dx.doi.org/10.1038/s41598-021-03325-5

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