Isolation and culturing of primary mouse astrocytes for the analysis of focal adhesion dynamics

Primary astrocytes have gained attention as an important model for in vitro biological and biochemical research in the last decades. In this protocol, we describe a fast and cost-effective technique for isolating, culturing, and maintaining primary mouse astrocytes at ∼ 80% purity levels, which can...

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Autores principales: Güler, Baran E., Krzysko, Jacek, Wolfrum, Uwe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669101/
https://www.ncbi.nlm.nih.gov/pubmed/34917973
http://dx.doi.org/10.1016/j.xpro.2021.100954
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author Güler, Baran E.
Krzysko, Jacek
Wolfrum, Uwe
author_facet Güler, Baran E.
Krzysko, Jacek
Wolfrum, Uwe
author_sort Güler, Baran E.
collection PubMed
description Primary astrocytes have gained attention as an important model for in vitro biological and biochemical research in the last decades. In this protocol, we describe a fast and cost-effective technique for isolating, culturing, and maintaining primary mouse astrocytes at ∼ 80% purity levels, which can be used in in vitro studies for migration and focal adhesion dynamics. In addition, we present an optimized transfection and manual quantification approach for focal adhesion analysis in fixed and living cells. For complete details on the use and execution of this protocol, please refer to Kusuluri et al. (2021).
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spelling pubmed-86691012021-12-15 Isolation and culturing of primary mouse astrocytes for the analysis of focal adhesion dynamics Güler, Baran E. Krzysko, Jacek Wolfrum, Uwe STAR Protoc Protocol Primary astrocytes have gained attention as an important model for in vitro biological and biochemical research in the last decades. In this protocol, we describe a fast and cost-effective technique for isolating, culturing, and maintaining primary mouse astrocytes at ∼ 80% purity levels, which can be used in in vitro studies for migration and focal adhesion dynamics. In addition, we present an optimized transfection and manual quantification approach for focal adhesion analysis in fixed and living cells. For complete details on the use and execution of this protocol, please refer to Kusuluri et al. (2021). Elsevier 2021-12-08 /pmc/articles/PMC8669101/ /pubmed/34917973 http://dx.doi.org/10.1016/j.xpro.2021.100954 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Güler, Baran E.
Krzysko, Jacek
Wolfrum, Uwe
Isolation and culturing of primary mouse astrocytes for the analysis of focal adhesion dynamics
title Isolation and culturing of primary mouse astrocytes for the analysis of focal adhesion dynamics
title_full Isolation and culturing of primary mouse astrocytes for the analysis of focal adhesion dynamics
title_fullStr Isolation and culturing of primary mouse astrocytes for the analysis of focal adhesion dynamics
title_full_unstemmed Isolation and culturing of primary mouse astrocytes for the analysis of focal adhesion dynamics
title_short Isolation and culturing of primary mouse astrocytes for the analysis of focal adhesion dynamics
title_sort isolation and culturing of primary mouse astrocytes for the analysis of focal adhesion dynamics
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669101/
https://www.ncbi.nlm.nih.gov/pubmed/34917973
http://dx.doi.org/10.1016/j.xpro.2021.100954
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