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Isolation and culturing of primary mouse astrocytes for the analysis of focal adhesion dynamics

Primary astrocytes have gained attention as an important model for in vitro biological and biochemical research in the last decades. In this protocol, we describe a fast and cost-effective technique for isolating, culturing, and maintaining primary mouse astrocytes at ∼ 80% purity levels, which can...

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Detalles Bibliográficos
Autores principales: Güler, Baran E., Krzysko, Jacek, Wolfrum, Uwe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669101/
https://www.ncbi.nlm.nih.gov/pubmed/34917973
http://dx.doi.org/10.1016/j.xpro.2021.100954