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Isolation and culturing of primary mouse astrocytes for the analysis of focal adhesion dynamics
Primary astrocytes have gained attention as an important model for in vitro biological and biochemical research in the last decades. In this protocol, we describe a fast and cost-effective technique for isolating, culturing, and maintaining primary mouse astrocytes at ∼ 80% purity levels, which can...
Autores principales: | Güler, Baran E., Krzysko, Jacek, Wolfrum, Uwe |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669101/ https://www.ncbi.nlm.nih.gov/pubmed/34917973 http://dx.doi.org/10.1016/j.xpro.2021.100954 |
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