Cargando…

Development of an In-House Rapid Antimicrobial Susceptibility Testing Protocol for Positive Blood Culture and Its Implementation in Routine Microbiology Laboratories

Bloodstream infections (BSI) are associated with high morbidity and mortality and remain a leading cause of death. Blood culture (BC) including the identification and the antimicrobial susceptibility testing of the causative microorganisms should be performed as soon as possible. In this study, we d...

Descripción completa

Detalles Bibliográficos
Autores principales: Cao, Min, Huang, Lin, Hu, Yanyan, Fang, Yinfei, Zhang, Rong, Chen, Gongxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669140/
https://www.ncbi.nlm.nih.gov/pubmed/34917051
http://dx.doi.org/10.3389/fmicb.2021.765757
_version_ 1784614726529974272
author Cao, Min
Huang, Lin
Hu, Yanyan
Fang, Yinfei
Zhang, Rong
Chen, Gongxiang
author_facet Cao, Min
Huang, Lin
Hu, Yanyan
Fang, Yinfei
Zhang, Rong
Chen, Gongxiang
author_sort Cao, Min
collection PubMed
description Bloodstream infections (BSI) are associated with high morbidity and mortality and remain a leading cause of death. Blood culture (BC) including the identification and the antimicrobial susceptibility testing of the causative microorganisms should be performed as soon as possible. In this study, we developed an in-house rapid antimicrobial susceptibility testing (rAST) protocol for positive BC. First, the rAST was performed in the simulated positive BC of standard strains (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, and Pseudomonas aeruginosa ATCC 27853) at three different times to assess the reproducibility and operability by dispensing four drops of BC broth onto a Mueller–Hinton agar plate after a positive signal. Furthermore, the rAST was performed in clinical positive BCs. The results of rAST at 4, 6, 8, and 18 h of incubation were compared with results of the standard 16- to 20-h disk diffusion method, and the preliminary breakpoints of the rAST method were established according to the inhibition diameter of sensitive strains and resistant strains. Finally, the rAST was performed in the simulated positive BC of clinical strains to evaluate the availability of the preliminary breakpoints. The rAST results of standard strains were distributed evenly at three different times. Among the 202 clinical strains used to establish the preliminary breakpoints, the number of zone diameters that could be read and interpreted (60, 87, 98, and 100%) increased with incubation time (4, 6, 8, and 18 h), and the categorical agreement was acceptable, with total error rates of 3.0, 2.3, 2.1, and 1.3% at 4, 6, 8, and 18 h of incubation, respectively. In conclusion, the in-house rAST protocol for positive BC can be implemented in routine laboratories. It provides reliable antimicrobial susceptibility testing results for BSI pathogens after 4–6 h of incubation.
format Online
Article
Text
id pubmed-8669140
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-86691402021-12-15 Development of an In-House Rapid Antimicrobial Susceptibility Testing Protocol for Positive Blood Culture and Its Implementation in Routine Microbiology Laboratories Cao, Min Huang, Lin Hu, Yanyan Fang, Yinfei Zhang, Rong Chen, Gongxiang Front Microbiol Microbiology Bloodstream infections (BSI) are associated with high morbidity and mortality and remain a leading cause of death. Blood culture (BC) including the identification and the antimicrobial susceptibility testing of the causative microorganisms should be performed as soon as possible. In this study, we developed an in-house rapid antimicrobial susceptibility testing (rAST) protocol for positive BC. First, the rAST was performed in the simulated positive BC of standard strains (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, and Pseudomonas aeruginosa ATCC 27853) at three different times to assess the reproducibility and operability by dispensing four drops of BC broth onto a Mueller–Hinton agar plate after a positive signal. Furthermore, the rAST was performed in clinical positive BCs. The results of rAST at 4, 6, 8, and 18 h of incubation were compared with results of the standard 16- to 20-h disk diffusion method, and the preliminary breakpoints of the rAST method were established according to the inhibition diameter of sensitive strains and resistant strains. Finally, the rAST was performed in the simulated positive BC of clinical strains to evaluate the availability of the preliminary breakpoints. The rAST results of standard strains were distributed evenly at three different times. Among the 202 clinical strains used to establish the preliminary breakpoints, the number of zone diameters that could be read and interpreted (60, 87, 98, and 100%) increased with incubation time (4, 6, 8, and 18 h), and the categorical agreement was acceptable, with total error rates of 3.0, 2.3, 2.1, and 1.3% at 4, 6, 8, and 18 h of incubation, respectively. In conclusion, the in-house rAST protocol for positive BC can be implemented in routine laboratories. It provides reliable antimicrobial susceptibility testing results for BSI pathogens after 4–6 h of incubation. Frontiers Media S.A. 2021-11-30 /pmc/articles/PMC8669140/ /pubmed/34917051 http://dx.doi.org/10.3389/fmicb.2021.765757 Text en Copyright © 2021 Cao, Huang, Hu, Fang, Zhang and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Cao, Min
Huang, Lin
Hu, Yanyan
Fang, Yinfei
Zhang, Rong
Chen, Gongxiang
Development of an In-House Rapid Antimicrobial Susceptibility Testing Protocol for Positive Blood Culture and Its Implementation in Routine Microbiology Laboratories
title Development of an In-House Rapid Antimicrobial Susceptibility Testing Protocol for Positive Blood Culture and Its Implementation in Routine Microbiology Laboratories
title_full Development of an In-House Rapid Antimicrobial Susceptibility Testing Protocol for Positive Blood Culture and Its Implementation in Routine Microbiology Laboratories
title_fullStr Development of an In-House Rapid Antimicrobial Susceptibility Testing Protocol for Positive Blood Culture and Its Implementation in Routine Microbiology Laboratories
title_full_unstemmed Development of an In-House Rapid Antimicrobial Susceptibility Testing Protocol for Positive Blood Culture and Its Implementation in Routine Microbiology Laboratories
title_short Development of an In-House Rapid Antimicrobial Susceptibility Testing Protocol for Positive Blood Culture and Its Implementation in Routine Microbiology Laboratories
title_sort development of an in-house rapid antimicrobial susceptibility testing protocol for positive blood culture and its implementation in routine microbiology laboratories
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669140/
https://www.ncbi.nlm.nih.gov/pubmed/34917051
http://dx.doi.org/10.3389/fmicb.2021.765757
work_keys_str_mv AT caomin developmentofaninhouserapidantimicrobialsusceptibilitytestingprotocolforpositivebloodcultureanditsimplementationinroutinemicrobiologylaboratories
AT huanglin developmentofaninhouserapidantimicrobialsusceptibilitytestingprotocolforpositivebloodcultureanditsimplementationinroutinemicrobiologylaboratories
AT huyanyan developmentofaninhouserapidantimicrobialsusceptibilitytestingprotocolforpositivebloodcultureanditsimplementationinroutinemicrobiologylaboratories
AT fangyinfei developmentofaninhouserapidantimicrobialsusceptibilitytestingprotocolforpositivebloodcultureanditsimplementationinroutinemicrobiologylaboratories
AT zhangrong developmentofaninhouserapidantimicrobialsusceptibilitytestingprotocolforpositivebloodcultureanditsimplementationinroutinemicrobiologylaboratories
AT chengongxiang developmentofaninhouserapidantimicrobialsusceptibilitytestingprotocolforpositivebloodcultureanditsimplementationinroutinemicrobiologylaboratories