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Reagents and models for detecting endogenous GLP1R and GIPR

Glucagon-like peptide-1 receptor (GLP1R) agonists target the GLP1R, whereas dual GLP1R/ gastric inhibitory polypeptide receptor (GIPR) agonists target both the GLP1R and GIPR. Despite the importance of these drug classes for the treatment of diabetes and obesity, still very little is known about the...

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Detalles Bibliográficos
Autores principales: Ast, Julia, Broichhagen, Johannes, Hodson, David J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669301/
https://www.ncbi.nlm.nih.gov/pubmed/34911028
http://dx.doi.org/10.1016/j.ebiom.2021.103739
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author Ast, Julia
Broichhagen, Johannes
Hodson, David J.
author_facet Ast, Julia
Broichhagen, Johannes
Hodson, David J.
author_sort Ast, Julia
collection PubMed
description Glucagon-like peptide-1 receptor (GLP1R) agonists target the GLP1R, whereas dual GLP1R/ gastric inhibitory polypeptide receptor (GIPR) agonists target both the GLP1R and GIPR. Despite the importance of these drug classes for the treatment of diabetes and obesity, still very little is known about the localization of GLP1R and GIPR themselves. Complicating matters is the low abundance of GLP1R and GIPR mRNA/protein, as well as a lack of specific and validated reagents for their detection. Without knowing where GLP1R and GIPR are located, it is difficult to propose mechanisms of action in the various target organs, and whether this is indirect or direct. In the current review, we will explain the steps needed to properly validate reagents for endogenous GLP1R/GIPR detection, describe the available approaches to visualize GLP1R/GIPR, and provide an update on the state-of-art. The overall aim is to provide a reference resource for researchers interested in GLP1R and GIPR signaling.
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spelling pubmed-86693012021-12-15 Reagents and models for detecting endogenous GLP1R and GIPR Ast, Julia Broichhagen, Johannes Hodson, David J. EBioMedicine Review Glucagon-like peptide-1 receptor (GLP1R) agonists target the GLP1R, whereas dual GLP1R/ gastric inhibitory polypeptide receptor (GIPR) agonists target both the GLP1R and GIPR. Despite the importance of these drug classes for the treatment of diabetes and obesity, still very little is known about the localization of GLP1R and GIPR themselves. Complicating matters is the low abundance of GLP1R and GIPR mRNA/protein, as well as a lack of specific and validated reagents for their detection. Without knowing where GLP1R and GIPR are located, it is difficult to propose mechanisms of action in the various target organs, and whether this is indirect or direct. In the current review, we will explain the steps needed to properly validate reagents for endogenous GLP1R/GIPR detection, describe the available approaches to visualize GLP1R/GIPR, and provide an update on the state-of-art. The overall aim is to provide a reference resource for researchers interested in GLP1R and GIPR signaling. Elsevier 2021-12-12 /pmc/articles/PMC8669301/ /pubmed/34911028 http://dx.doi.org/10.1016/j.ebiom.2021.103739 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Ast, Julia
Broichhagen, Johannes
Hodson, David J.
Reagents and models for detecting endogenous GLP1R and GIPR
title Reagents and models for detecting endogenous GLP1R and GIPR
title_full Reagents and models for detecting endogenous GLP1R and GIPR
title_fullStr Reagents and models for detecting endogenous GLP1R and GIPR
title_full_unstemmed Reagents and models for detecting endogenous GLP1R and GIPR
title_short Reagents and models for detecting endogenous GLP1R and GIPR
title_sort reagents and models for detecting endogenous glp1r and gipr
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669301/
https://www.ncbi.nlm.nih.gov/pubmed/34911028
http://dx.doi.org/10.1016/j.ebiom.2021.103739
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