Simultaneous recording of behavioral and neural responses of free-moving nematodes C. elegans

To reveal the neural mechanisms that control animal behavior, it is necessary to link the neural responses to behavioral changes and interpret them. We have developed a protocol to simultaneously record the behavior and neural activity of freely moving C. elegans by combining a microfluidic device a...

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Detalles Bibliográficos
Autores principales: Sato, Hirofumi, Kunitomo, Hirofumi, Fei, Xianfeng, Hashimoto, Koichi, Iino, Yuichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669343/
https://www.ncbi.nlm.nih.gov/pubmed/34917983
http://dx.doi.org/10.1016/j.xpro.2021.101011
Descripción
Sumario:To reveal the neural mechanisms that control animal behavior, it is necessary to link the neural responses to behavioral changes and interpret them. We have developed a protocol to simultaneously record the behavior and neural activity of freely moving C. elegans by combining a microfluidic device and a tracking stage. Here we detail the protocol for the experiment, with an example of behavioral and neural responses of nematodes to salt concentration changes. For complete details on the use and execution of this protocol, please refer to Sato et al. (2021).

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