Simultaneous recording of behavioral and neural responses of free-moving nematodes C. elegans

To reveal the neural mechanisms that control animal behavior, it is necessary to link the neural responses to behavioral changes and interpret them. We have developed a protocol to simultaneously record the behavior and neural activity of freely moving C. elegans by combining a microfluidic device a...

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Detalles Bibliográficos
Autores principales: Sato, Hirofumi, Kunitomo, Hirofumi, Fei, Xianfeng, Hashimoto, Koichi, Iino, Yuichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669343/
https://www.ncbi.nlm.nih.gov/pubmed/34917983
http://dx.doi.org/10.1016/j.xpro.2021.101011
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author Sato, Hirofumi
Kunitomo, Hirofumi
Fei, Xianfeng
Hashimoto, Koichi
Iino, Yuichi
author_facet Sato, Hirofumi
Kunitomo, Hirofumi
Fei, Xianfeng
Hashimoto, Koichi
Iino, Yuichi
author_sort Sato, Hirofumi
collection PubMed
description To reveal the neural mechanisms that control animal behavior, it is necessary to link the neural responses to behavioral changes and interpret them. We have developed a protocol to simultaneously record the behavior and neural activity of freely moving C. elegans by combining a microfluidic device and a tracking stage. Here we detail the protocol for the experiment, with an example of behavioral and neural responses of nematodes to salt concentration changes. For complete details on the use and execution of this protocol, please refer to Sato et al. (2021).
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spelling pubmed-86693432021-12-15 Simultaneous recording of behavioral and neural responses of free-moving nematodes C. elegans Sato, Hirofumi Kunitomo, Hirofumi Fei, Xianfeng Hashimoto, Koichi Iino, Yuichi STAR Protoc Protocol To reveal the neural mechanisms that control animal behavior, it is necessary to link the neural responses to behavioral changes and interpret them. We have developed a protocol to simultaneously record the behavior and neural activity of freely moving C. elegans by combining a microfluidic device and a tracking stage. Here we detail the protocol for the experiment, with an example of behavioral and neural responses of nematodes to salt concentration changes. For complete details on the use and execution of this protocol, please refer to Sato et al. (2021). Elsevier 2021-12-13 /pmc/articles/PMC8669343/ /pubmed/34917983 http://dx.doi.org/10.1016/j.xpro.2021.101011 Text en © 2021. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Sato, Hirofumi
Kunitomo, Hirofumi
Fei, Xianfeng
Hashimoto, Koichi
Iino, Yuichi
Simultaneous recording of behavioral and neural responses of free-moving nematodes C. elegans
title Simultaneous recording of behavioral and neural responses of free-moving nematodes C. elegans
title_full Simultaneous recording of behavioral and neural responses of free-moving nematodes C. elegans
title_fullStr Simultaneous recording of behavioral and neural responses of free-moving nematodes C. elegans
title_full_unstemmed Simultaneous recording of behavioral and neural responses of free-moving nematodes C. elegans
title_short Simultaneous recording of behavioral and neural responses of free-moving nematodes C. elegans
title_sort simultaneous recording of behavioral and neural responses of free-moving nematodes c. elegans
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669343/
https://www.ncbi.nlm.nih.gov/pubmed/34917983
http://dx.doi.org/10.1016/j.xpro.2021.101011
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