Cargando…

Combined Effects of Proton Radiation and Simulated Microgravity on the Cell Viability and ALP Activity of Murine Osteoblast Cells

Proton radiation (PR) and microgravity (μG) are two key factors that impact living things in space. This study aimed to explore the combined effects of PR and simulated μG (SμG) on bone function. Mouse embryo osteoblast precursor cells (MC3T3-E1) were irradiated with proton beams and immediately tre...

Descripción completa

Detalles Bibliográficos
Autores principales: Ma, Liqiu, Kong, Fuquan, Gong, Yihao, Wang, Qiaojuan, Liu, Jiancheng, Sui, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669367/
https://www.ncbi.nlm.nih.gov/pubmed/34917576
http://dx.doi.org/10.3389/fpubh.2021.759236
_version_ 1784614764566020096
author Ma, Liqiu
Kong, Fuquan
Gong, Yihao
Wang, Qiaojuan
Liu, Jiancheng
Sui, Li
author_facet Ma, Liqiu
Kong, Fuquan
Gong, Yihao
Wang, Qiaojuan
Liu, Jiancheng
Sui, Li
author_sort Ma, Liqiu
collection PubMed
description Proton radiation (PR) and microgravity (μG) are two key factors that impact living things in space. This study aimed to explore the combined effects of PR and simulated μG (SμG) on bone function. Mouse embryo osteoblast precursor cells (MC3T3-E1) were irradiated with proton beams and immediately treated with SμG for 2 days using a three-dimensional clinostat. All samples were subjected to cell viability, alkaline phosphatase (ALP) activity and transcriptome assays. The results showed that cell viability decreased with increasing doses of PR. The peak ALP activity after PR or SμG alone was lower than that obtained with the non-treatment control. No difference in cell viability or ALP activity was found between 1 Gy PR combined with SμG (PR-SμG) and PR alone. However, 4 Gy PR-SμG resulted in decreased cell viability and ALP activity compared with those obtained with PR alone. Furthermore, Gene Ontology analysis revealed the same trend. These results revealed that PR-SμG may lead to reductions in the proliferation and differentiation capacities of cells in a dose-dependent manner. Our data provide new insights into bone-related hazards caused by multiple factors, such as PR and μG, in the space environment.
format Online
Article
Text
id pubmed-8669367
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-86693672021-12-15 Combined Effects of Proton Radiation and Simulated Microgravity on the Cell Viability and ALP Activity of Murine Osteoblast Cells Ma, Liqiu Kong, Fuquan Gong, Yihao Wang, Qiaojuan Liu, Jiancheng Sui, Li Front Public Health Public Health Proton radiation (PR) and microgravity (μG) are two key factors that impact living things in space. This study aimed to explore the combined effects of PR and simulated μG (SμG) on bone function. Mouse embryo osteoblast precursor cells (MC3T3-E1) were irradiated with proton beams and immediately treated with SμG for 2 days using a three-dimensional clinostat. All samples were subjected to cell viability, alkaline phosphatase (ALP) activity and transcriptome assays. The results showed that cell viability decreased with increasing doses of PR. The peak ALP activity after PR or SμG alone was lower than that obtained with the non-treatment control. No difference in cell viability or ALP activity was found between 1 Gy PR combined with SμG (PR-SμG) and PR alone. However, 4 Gy PR-SμG resulted in decreased cell viability and ALP activity compared with those obtained with PR alone. Furthermore, Gene Ontology analysis revealed the same trend. These results revealed that PR-SμG may lead to reductions in the proliferation and differentiation capacities of cells in a dose-dependent manner. Our data provide new insights into bone-related hazards caused by multiple factors, such as PR and μG, in the space environment. Frontiers Media S.A. 2021-11-30 /pmc/articles/PMC8669367/ /pubmed/34917576 http://dx.doi.org/10.3389/fpubh.2021.759236 Text en Copyright © 2021 Ma, Kong, Gong, Wang, Liu and Sui. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Public Health
Ma, Liqiu
Kong, Fuquan
Gong, Yihao
Wang, Qiaojuan
Liu, Jiancheng
Sui, Li
Combined Effects of Proton Radiation and Simulated Microgravity on the Cell Viability and ALP Activity of Murine Osteoblast Cells
title Combined Effects of Proton Radiation and Simulated Microgravity on the Cell Viability and ALP Activity of Murine Osteoblast Cells
title_full Combined Effects of Proton Radiation and Simulated Microgravity on the Cell Viability and ALP Activity of Murine Osteoblast Cells
title_fullStr Combined Effects of Proton Radiation and Simulated Microgravity on the Cell Viability and ALP Activity of Murine Osteoblast Cells
title_full_unstemmed Combined Effects of Proton Radiation and Simulated Microgravity on the Cell Viability and ALP Activity of Murine Osteoblast Cells
title_short Combined Effects of Proton Radiation and Simulated Microgravity on the Cell Viability and ALP Activity of Murine Osteoblast Cells
title_sort combined effects of proton radiation and simulated microgravity on the cell viability and alp activity of murine osteoblast cells
topic Public Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669367/
https://www.ncbi.nlm.nih.gov/pubmed/34917576
http://dx.doi.org/10.3389/fpubh.2021.759236
work_keys_str_mv AT maliqiu combinedeffectsofprotonradiationandsimulatedmicrogravityonthecellviabilityandalpactivityofmurineosteoblastcells
AT kongfuquan combinedeffectsofprotonradiationandsimulatedmicrogravityonthecellviabilityandalpactivityofmurineosteoblastcells
AT gongyihao combinedeffectsofprotonradiationandsimulatedmicrogravityonthecellviabilityandalpactivityofmurineosteoblastcells
AT wangqiaojuan combinedeffectsofprotonradiationandsimulatedmicrogravityonthecellviabilityandalpactivityofmurineosteoblastcells
AT liujiancheng combinedeffectsofprotonradiationandsimulatedmicrogravityonthecellviabilityandalpactivityofmurineosteoblastcells
AT suili combinedeffectsofprotonradiationandsimulatedmicrogravityonthecellviabilityandalpactivityofmurineosteoblastcells