Cargando…

Green Process: Improved Semi-Continuous Fermentation of Pichia pastoris Based on the Principle of Vitality Cell Separation

The large-scale fermentation of Pichia pastoris for recombinant protein production would be time consuming and produce a large amount of waste yeast. Here we introduce a novel semi-continuous fermentation process for P. pastoris GS115 that can separate vitality cells from broth and recycle the cells...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wang, Denggang, Li, Wenjie, Zhang, Xinying, Liang, Shuli, Lin, Ying
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2021
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669635/ https://www.ncbi.nlm.nih.gov/pubmed/34917600 http://dx.doi.org/10.3389/fbioe.2021.777774

Ejemplares similares

Improving the catalytic performance of Pichia pastoris whole-cell biocatalysts by fermentation process
por: Wang, Denggang, et al.
Publicado: (2021)

Continuous Cultivation as a Tool Toward the Rational Bioprocess Development With Pichia Pastoris Cell Factory
por: Nieto-Taype, Miguel Angel, et al.
Publicado: (2020)

Cell Surface Display of Thermomyces lanuginosus Lipase in Pichia pastoris
por: Yang, Jiaming, et al.
Publicado: (2020)

Artificial Transcription Factors for Tuneable Gene Expression in Pichia pastoris
por: Naseri, Gita, et al.
Publicado: (2021)

Polycistronic Expression System for Pichia pastoris Composed of Chitino- and Chitosanolytic Enzymes
por: Kaczmarek, Michal B., et al.
Publicado: (2021)

Establishing a Eukaryotic Pichia pastoris Cell-Free Protein Synthesis System
por: Zhang, Lingkai, et al.
Publicado: (2020)

A Combinational Strategy for Effective Heterologous Production of Functional Human Lysozyme in Pichia pastoris
por: He, Huahua, et al.
Publicado: (2020)

Improved Production of Xylanase in Pichia pastoris and Its Application in Xylose Production From Xylan
por: Miao, Ting, et al.
Publicado: (2021)

High-Level Expression and Biochemical Properties of A Thermo-Alkaline Pectate Lyase From Bacillus sp. RN1 in Pichia pastoris With Potential in Ramie Degumming
por: Zheng, Xueyun, et al.
Publicado: (2020)

Combining Metabolic Engineering and Multiplexed Screening Methods for 3-Hydroxypropionic Acid Production in Pichia pastoris
por: Fina, Albert, et al.
Publicado: (2022)

Fed-Batch Production of Saccharomyces cerevisiae L-Asparaginase II by Recombinant Pichia pastoris MUT(s) Strain
por: Rodrigues, David, et al.
Publicado: (2019)

Synthesis and Assembly of Hepatitis B Virus-Like Particles in a Pichia pastoris Cell-Free System
por: Spice, Alex J., et al.
Publicado: (2020)

Corrigendum: Combining metabolic engineering and multiplexed screening methods for 3-hydroxypropionic acid production in Pichia pastoris
por: Fina, Albert, et al.
Publicado: (2022)

P( AOX1 ) expression in mixed-substrate continuous cultures of Komagataella phaffii (Pichia pastoris) is completely determined by methanol consumption regardless of the secondary carbon source
por: Singh, Anamika, et al.
Publicado: (2023)

Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production
por: Gasset, Arnau, et al.
Publicado: (2022)

Improved production of recombinant β-mannanase (TaMan5) in Pichia pastoris and its synergistic degradation of lignocellulosic biomass
por: Zheng, Fengzhen, et al.
Publicado: (2023)

Overall Key Performance Indicator to Optimizing Operation of High-Pressure Homogenizers for a Reliable Quantification of Intracellular Components in Pichia pastoris
por: Garcia-Ortega, Xavier, et al.
Publicado: (2015)

Gene cloning and expression of MAP30 in Pichia pastoris
por: Wang, Fang, et al.
Publicado: (2014)

Transcriptional Regulation of Aerobic Metabolism in Pichia pastoris Fermentation
por: Zhang, Biao, et al.
Publicado: (2016)

Production of recombinant human epidermal growth factor in Pichia pastoris
por: Eissazadeh, Samira, et al.
Publicado: (2017)

Secreted production of an elastin-like polypeptide by Pichia pastoris
por: Schipperus, Roelof, et al.
Publicado: (2009)

A Downstream Processing Cascade for Separation of Caproic and Caprylic Acid from Maize Silage-Based Fermentation Broth
por: Braune, Maria, et al.
Publicado: (2021)

Corrigendum: A downstream processing cascade for separation of caproic and caprylic acid from maize silage-based fermentation broth
por: Braune, Maria, et al.
Publicado: (2022)

Recycling of a selectable marker with a self-excisable plasmid in Pichia pastoris
por: Li, Cheng, et al.
Publicado: (2017)

Separation, purification, and crystallization of 1,5-pentanediamine hydrochloride from fermentation broth by cation resin
por: Li, Hui, et al.
Publicado: (2023)

Pichia pastoris Exhibits High Viability and a Low Maintenance Energy Requirement at Near-Zero Specific Growth Rates
por: Rebnegger, Corinna, et al.
Publicado: (2016)

Continuous Self-Cycling Fermentation Leads to Economical Lycopene Production by Saccharomyces cerevisiae
por: Wang, Zhiming, et al.
Publicado: (2020)

Improving Thermostability and Catalytic Activity of Glycosyltransferase From Panax ginseng by Semi-Rational Design for Rebaudioside D Synthesis
por: Chen, Meiqi, et al.
Publicado: (2022)

Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris
por: Sygmund, Christoph, et al.
Publicado: (2011)

Production and secretion dynamics of prokaryotic Penicillin G acylase in Pichia pastoris
por: Borčinová, Martina, et al.
Publicado: (2020)

High Productivity of Eicosapentaenoic Acid and Fucoxanthin by a Marine Diatom Chaetoceros gracilis in a Semi-Continuous Culture
por: Tachihana, Saki, et al.
Publicado: (2020)

Pulsed electric field: A “green” extraction technology for biomolecular products from glycerol with fermentation of non-Saccharomyces yeasts
por: Tsapou, Evangelia A., et al.
Publicado: (2022)

Fhl1p protein, a positive transcription factor in Pichia pastoris, enhances the expression of recombinant proteins
por: Zheng, Xueyun, et al.
Publicado: (2019)

The optimization of fermentation conditions for Pichia pastoris GS115 producing recombinant xylanase
por: Sun, Taotao, et al.
Publicado: (2020)

Development and Optimization of a Novel Soft Sensor Modeling Method for Fermentation Process of Pichia pastoris
por: Wang, Bo, et al.
Publicado: (2023)

Bromination of L-tryptophan in a Fermentative Process With Corynebacterium glutamicum
por: Veldmann, Kareen H., et al.
Publicado: (2019)

Two-Stage Semi-Continuous 2-Keto-Gluconic Acid (2KGA) Production by Pseudomonas plecoglossicida JUIM01 From Rice Starch Hydrolyzate
por: Sun, Lei, et al.
Publicado: (2020)

Exchange of endogenous and heterogeneous yeast terminators in Pichia pastoris to tune mRNA stability and gene expression
por: Ito, Yoichiro, et al.
Publicado: (2020)

Construction and screening of a glycosylphosphatidylinositol protein deletion library in Pichia pastoris
por: Wang, Pan, et al.
Publicado: (2020)

Effects of adding cocoa fermentation medium on cigar leaves in agricultural fermentation stage
por: Zong, Pengfei, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_5msbcl80bb2nloj23lomc6jnng