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MicroRNA-671-5p inhibits cell proliferation, migration and invasion in non-small cell lung cancer by targeting MFAP3L
MicroRNA (miR)-671-5p serves as a tumor suppressor in several types of cancer, including gastric and breast cancer. However, the function of miR-671-5p in non-small cell lung cancer (NSCLC) has not been described in detail. The present study aimed to investigate the role of miR-671-5p in NSCLC. The...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669681/ https://www.ncbi.nlm.nih.gov/pubmed/34841435 http://dx.doi.org/10.3892/mmr.2021.12546 |
Sumario: | MicroRNA (miR)-671-5p serves as a tumor suppressor in several types of cancer, including gastric and breast cancer. However, the function of miR-671-5p in non-small cell lung cancer (NSCLC) has not been described in detail. The present study aimed to investigate the role of miR-671-5p in NSCLC. The expression levels of miR-671-5p were determined in NSCLC tissue samples and cell lines using reverse transcription-quantitative PCR. Prediction of miR-671-5p targets was performed using the TargetScan database and verified by luciferase reporter assay and western blot analysis. Functional experiments, including Cell Counting Kit-8, wound healing and Transwell assays, were performed in NSCLC cells. The results of the present study demonstrated that lower expression levels of miR-671-5p were observed in NSCLC tissues and cell lines compared with those in the corresponding controls. Low miR-671-5p levels were significantly associated with an advanced Tumor-Node-Metastasis stage and lymph node metastasis in patients with NSCLC. Microfibril-associated protein 3-like (MFAP3L) was confirmed to be a direct target of miR-671-5p. The proliferative, migratory and invasive abilities of NSCLC cells were suppressed following transfection with miR-671-5p mimics and promoted by the miR-671-5p inhibitor compared with those in the respective control groups. In addition, the effects of miR-671-5p on cell proliferation, migration and invasion, as well as the expression levels of proliferating cell nuclear antigen, E-cadherin, N-cadherin and vimentin were reversed by MFAP3L overexpression. In conclusion, targeting the miR-671-5p/MFAP3L signaling pathway may be a promising therapeutic strategy for NSCLC treatment. |
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