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Potential anticancer effect of aspirin and 2′-hydroxy-2,3,5′-trimethoxychalcone-linked polymeric micelles against cervical cancer through apoptosis

Although early diagnosis and treatment of cancers in women are achievable through continuous diagnostic tests, cervical cancer (CVC) still has a high mortality rate. In the present study, we investigated whether certain nanoparticles (NPs), comprising aspirin conjugated 2′-hydroxy-2,3,5′-trimethoxyc...

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Autores principales: Lee, Kang Pa, Baek, Suji, Yoon, Myeong Sik, Park, Ji Soo, Hong, Bok Sil, Lee, Sang Ju, Oh, Seung Jun, Kwon, Seung Hae, Lee, Ruda, Lee, Dae Ho, Park, Kang-Seo, Moon, Byung Seok
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669688/
https://www.ncbi.nlm.nih.gov/pubmed/34966447
http://dx.doi.org/10.3892/ol.2021.13149
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author Lee, Kang Pa
Baek, Suji
Yoon, Myeong Sik
Park, Ji Soo
Hong, Bok Sil
Lee, Sang Ju
Oh, Seung Jun
Kwon, Seung Hae
Lee, Ruda
Lee, Dae Ho
Park, Kang-Seo
Moon, Byung Seok
author_facet Lee, Kang Pa
Baek, Suji
Yoon, Myeong Sik
Park, Ji Soo
Hong, Bok Sil
Lee, Sang Ju
Oh, Seung Jun
Kwon, Seung Hae
Lee, Ruda
Lee, Dae Ho
Park, Kang-Seo
Moon, Byung Seok
author_sort Lee, Kang Pa
collection PubMed
description Although early diagnosis and treatment of cancers in women are achievable through continuous diagnostic tests, cervical cancer (CVC) still has a high mortality rate. In the present study, we investigated whether certain nanoparticles (NPs), comprising aspirin conjugated 2′-hydroxy-2,3,5′-trimethoxychalcone chemicals, could induce the apoptosis of cancer cells. HeLa cells were treated with NPs and the cell viability was evaluated using WST-1 assay. Protein expression of Ki-67 was measured using immunocytochemistry. In addition, the apoptotic effect of NPs was determined using TUNEL assay. To investigate the apoptosis signaling pathways, reverse transcription quantitative PCR was performed and lipid accumulation was observed via holotomographic microscopy. The IC(50) value of the NPs was 4.172 µM in HeLa cells. Furthermore, 10 µM NPs significantly inhibited the cell proliferation and stimulated the apoptosis of HeLa cells. In addition, apoptosis and mitochondrial dysfunction were induced by the NPs through lipid accumulation in HeLa cells, leading to apoptotic signaling cascades. Taken together, the results from the present study demonstrated that the NPs developed promoted apoptosis though efficient lipid accumulation in HeLa cells, suggesting that they may provide a novel way to improve the efficacy of CVC anticancer treatment.
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spelling pubmed-86696882021-12-28 Potential anticancer effect of aspirin and 2′-hydroxy-2,3,5′-trimethoxychalcone-linked polymeric micelles against cervical cancer through apoptosis Lee, Kang Pa Baek, Suji Yoon, Myeong Sik Park, Ji Soo Hong, Bok Sil Lee, Sang Ju Oh, Seung Jun Kwon, Seung Hae Lee, Ruda Lee, Dae Ho Park, Kang-Seo Moon, Byung Seok Oncol Lett Articles Although early diagnosis and treatment of cancers in women are achievable through continuous diagnostic tests, cervical cancer (CVC) still has a high mortality rate. In the present study, we investigated whether certain nanoparticles (NPs), comprising aspirin conjugated 2′-hydroxy-2,3,5′-trimethoxychalcone chemicals, could induce the apoptosis of cancer cells. HeLa cells were treated with NPs and the cell viability was evaluated using WST-1 assay. Protein expression of Ki-67 was measured using immunocytochemistry. In addition, the apoptotic effect of NPs was determined using TUNEL assay. To investigate the apoptosis signaling pathways, reverse transcription quantitative PCR was performed and lipid accumulation was observed via holotomographic microscopy. The IC(50) value of the NPs was 4.172 µM in HeLa cells. Furthermore, 10 µM NPs significantly inhibited the cell proliferation and stimulated the apoptosis of HeLa cells. In addition, apoptosis and mitochondrial dysfunction were induced by the NPs through lipid accumulation in HeLa cells, leading to apoptotic signaling cascades. Taken together, the results from the present study demonstrated that the NPs developed promoted apoptosis though efficient lipid accumulation in HeLa cells, suggesting that they may provide a novel way to improve the efficacy of CVC anticancer treatment. D.A. Spandidos 2022-01 2021-11-25 /pmc/articles/PMC8669688/ /pubmed/34966447 http://dx.doi.org/10.3892/ol.2021.13149 Text en Copyright: © Lee et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Lee, Kang Pa
Baek, Suji
Yoon, Myeong Sik
Park, Ji Soo
Hong, Bok Sil
Lee, Sang Ju
Oh, Seung Jun
Kwon, Seung Hae
Lee, Ruda
Lee, Dae Ho
Park, Kang-Seo
Moon, Byung Seok
Potential anticancer effect of aspirin and 2′-hydroxy-2,3,5′-trimethoxychalcone-linked polymeric micelles against cervical cancer through apoptosis
title Potential anticancer effect of aspirin and 2′-hydroxy-2,3,5′-trimethoxychalcone-linked polymeric micelles against cervical cancer through apoptosis
title_full Potential anticancer effect of aspirin and 2′-hydroxy-2,3,5′-trimethoxychalcone-linked polymeric micelles against cervical cancer through apoptosis
title_fullStr Potential anticancer effect of aspirin and 2′-hydroxy-2,3,5′-trimethoxychalcone-linked polymeric micelles against cervical cancer through apoptosis
title_full_unstemmed Potential anticancer effect of aspirin and 2′-hydroxy-2,3,5′-trimethoxychalcone-linked polymeric micelles against cervical cancer through apoptosis
title_short Potential anticancer effect of aspirin and 2′-hydroxy-2,3,5′-trimethoxychalcone-linked polymeric micelles against cervical cancer through apoptosis
title_sort potential anticancer effect of aspirin and 2′-hydroxy-2,3,5′-trimethoxychalcone-linked polymeric micelles against cervical cancer through apoptosis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669688/
https://www.ncbi.nlm.nih.gov/pubmed/34966447
http://dx.doi.org/10.3892/ol.2021.13149
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