Cargando…
Potential anticancer effect of aspirin and 2′-hydroxy-2,3,5′-trimethoxychalcone-linked polymeric micelles against cervical cancer through apoptosis
Although early diagnosis and treatment of cancers in women are achievable through continuous diagnostic tests, cervical cancer (CVC) still has a high mortality rate. In the present study, we investigated whether certain nanoparticles (NPs), comprising aspirin conjugated 2′-hydroxy-2,3,5′-trimethoxyc...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669688/ https://www.ncbi.nlm.nih.gov/pubmed/34966447 http://dx.doi.org/10.3892/ol.2021.13149 |
_version_ | 1784614830055882752 |
---|---|
author | Lee, Kang Pa Baek, Suji Yoon, Myeong Sik Park, Ji Soo Hong, Bok Sil Lee, Sang Ju Oh, Seung Jun Kwon, Seung Hae Lee, Ruda Lee, Dae Ho Park, Kang-Seo Moon, Byung Seok |
author_facet | Lee, Kang Pa Baek, Suji Yoon, Myeong Sik Park, Ji Soo Hong, Bok Sil Lee, Sang Ju Oh, Seung Jun Kwon, Seung Hae Lee, Ruda Lee, Dae Ho Park, Kang-Seo Moon, Byung Seok |
author_sort | Lee, Kang Pa |
collection | PubMed |
description | Although early diagnosis and treatment of cancers in women are achievable through continuous diagnostic tests, cervical cancer (CVC) still has a high mortality rate. In the present study, we investigated whether certain nanoparticles (NPs), comprising aspirin conjugated 2′-hydroxy-2,3,5′-trimethoxychalcone chemicals, could induce the apoptosis of cancer cells. HeLa cells were treated with NPs and the cell viability was evaluated using WST-1 assay. Protein expression of Ki-67 was measured using immunocytochemistry. In addition, the apoptotic effect of NPs was determined using TUNEL assay. To investigate the apoptosis signaling pathways, reverse transcription quantitative PCR was performed and lipid accumulation was observed via holotomographic microscopy. The IC(50) value of the NPs was 4.172 µM in HeLa cells. Furthermore, 10 µM NPs significantly inhibited the cell proliferation and stimulated the apoptosis of HeLa cells. In addition, apoptosis and mitochondrial dysfunction were induced by the NPs through lipid accumulation in HeLa cells, leading to apoptotic signaling cascades. Taken together, the results from the present study demonstrated that the NPs developed promoted apoptosis though efficient lipid accumulation in HeLa cells, suggesting that they may provide a novel way to improve the efficacy of CVC anticancer treatment. |
format | Online Article Text |
id | pubmed-8669688 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-86696882021-12-28 Potential anticancer effect of aspirin and 2′-hydroxy-2,3,5′-trimethoxychalcone-linked polymeric micelles against cervical cancer through apoptosis Lee, Kang Pa Baek, Suji Yoon, Myeong Sik Park, Ji Soo Hong, Bok Sil Lee, Sang Ju Oh, Seung Jun Kwon, Seung Hae Lee, Ruda Lee, Dae Ho Park, Kang-Seo Moon, Byung Seok Oncol Lett Articles Although early diagnosis and treatment of cancers in women are achievable through continuous diagnostic tests, cervical cancer (CVC) still has a high mortality rate. In the present study, we investigated whether certain nanoparticles (NPs), comprising aspirin conjugated 2′-hydroxy-2,3,5′-trimethoxychalcone chemicals, could induce the apoptosis of cancer cells. HeLa cells were treated with NPs and the cell viability was evaluated using WST-1 assay. Protein expression of Ki-67 was measured using immunocytochemistry. In addition, the apoptotic effect of NPs was determined using TUNEL assay. To investigate the apoptosis signaling pathways, reverse transcription quantitative PCR was performed and lipid accumulation was observed via holotomographic microscopy. The IC(50) value of the NPs was 4.172 µM in HeLa cells. Furthermore, 10 µM NPs significantly inhibited the cell proliferation and stimulated the apoptosis of HeLa cells. In addition, apoptosis and mitochondrial dysfunction were induced by the NPs through lipid accumulation in HeLa cells, leading to apoptotic signaling cascades. Taken together, the results from the present study demonstrated that the NPs developed promoted apoptosis though efficient lipid accumulation in HeLa cells, suggesting that they may provide a novel way to improve the efficacy of CVC anticancer treatment. D.A. Spandidos 2022-01 2021-11-25 /pmc/articles/PMC8669688/ /pubmed/34966447 http://dx.doi.org/10.3892/ol.2021.13149 Text en Copyright: © Lee et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Lee, Kang Pa Baek, Suji Yoon, Myeong Sik Park, Ji Soo Hong, Bok Sil Lee, Sang Ju Oh, Seung Jun Kwon, Seung Hae Lee, Ruda Lee, Dae Ho Park, Kang-Seo Moon, Byung Seok Potential anticancer effect of aspirin and 2′-hydroxy-2,3,5′-trimethoxychalcone-linked polymeric micelles against cervical cancer through apoptosis |
title | Potential anticancer effect of aspirin and 2′-hydroxy-2,3,5′-trimethoxychalcone-linked polymeric micelles against cervical cancer through apoptosis |
title_full | Potential anticancer effect of aspirin and 2′-hydroxy-2,3,5′-trimethoxychalcone-linked polymeric micelles against cervical cancer through apoptosis |
title_fullStr | Potential anticancer effect of aspirin and 2′-hydroxy-2,3,5′-trimethoxychalcone-linked polymeric micelles against cervical cancer through apoptosis |
title_full_unstemmed | Potential anticancer effect of aspirin and 2′-hydroxy-2,3,5′-trimethoxychalcone-linked polymeric micelles against cervical cancer through apoptosis |
title_short | Potential anticancer effect of aspirin and 2′-hydroxy-2,3,5′-trimethoxychalcone-linked polymeric micelles against cervical cancer through apoptosis |
title_sort | potential anticancer effect of aspirin and 2′-hydroxy-2,3,5′-trimethoxychalcone-linked polymeric micelles against cervical cancer through apoptosis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669688/ https://www.ncbi.nlm.nih.gov/pubmed/34966447 http://dx.doi.org/10.3892/ol.2021.13149 |
work_keys_str_mv | AT leekangpa potentialanticancereffectofaspirinand2hydroxy235trimethoxychalconelinkedpolymericmicellesagainstcervicalcancerthroughapoptosis AT baeksuji potentialanticancereffectofaspirinand2hydroxy235trimethoxychalconelinkedpolymericmicellesagainstcervicalcancerthroughapoptosis AT yoonmyeongsik potentialanticancereffectofaspirinand2hydroxy235trimethoxychalconelinkedpolymericmicellesagainstcervicalcancerthroughapoptosis AT parkjisoo potentialanticancereffectofaspirinand2hydroxy235trimethoxychalconelinkedpolymericmicellesagainstcervicalcancerthroughapoptosis AT hongboksil potentialanticancereffectofaspirinand2hydroxy235trimethoxychalconelinkedpolymericmicellesagainstcervicalcancerthroughapoptosis AT leesangju potentialanticancereffectofaspirinand2hydroxy235trimethoxychalconelinkedpolymericmicellesagainstcervicalcancerthroughapoptosis AT ohseungjun potentialanticancereffectofaspirinand2hydroxy235trimethoxychalconelinkedpolymericmicellesagainstcervicalcancerthroughapoptosis AT kwonseunghae potentialanticancereffectofaspirinand2hydroxy235trimethoxychalconelinkedpolymericmicellesagainstcervicalcancerthroughapoptosis AT leeruda potentialanticancereffectofaspirinand2hydroxy235trimethoxychalconelinkedpolymericmicellesagainstcervicalcancerthroughapoptosis AT leedaeho potentialanticancereffectofaspirinand2hydroxy235trimethoxychalconelinkedpolymericmicellesagainstcervicalcancerthroughapoptosis AT parkkangseo potentialanticancereffectofaspirinand2hydroxy235trimethoxychalconelinkedpolymericmicellesagainstcervicalcancerthroughapoptosis AT moonbyungseok potentialanticancereffectofaspirinand2hydroxy235trimethoxychalconelinkedpolymericmicellesagainstcervicalcancerthroughapoptosis |