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Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study
Health monitoring during offshore saturation diving is complicated due to restricted access to the divers, the desire to keep invasive procedures to a minimum, and limited opportunity for laboratory work onboard dive support vessels (DSV). In this pilot study, we examined whether measuring salivary...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669759/ https://www.ncbi.nlm.nih.gov/pubmed/34916964 http://dx.doi.org/10.3389/fphys.2021.791525 |
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author | Monnoyer, Roxane Lautridou, Jacky Deb, Sanjoy Hjelde, Astrid Eftedal, Ingrid |
author_facet | Monnoyer, Roxane Lautridou, Jacky Deb, Sanjoy Hjelde, Astrid Eftedal, Ingrid |
author_sort | Monnoyer, Roxane |
collection | PubMed |
description | Health monitoring during offshore saturation diving is complicated due to restricted access to the divers, the desire to keep invasive procedures to a minimum, and limited opportunity for laboratory work onboard dive support vessels (DSV). In this pilot study, we examined whether measuring salivary biomarkrers in samples collected by the divers themselves might be a feasible approach to environmental stress assessment. Nine saturation divers were trained in the passive drool method for saliva collection and proceeded to collect samples at nine time points before, during, and after an offshore commercial saturation diving campaign. Samples collected within the hyperbaric living chambers were decompressed and stored frozen at −20°C onboard the DSV until they were shipped to land for analysis. Passive drool samples were collected without loss and assayed for a selection of salivary biomarkers: secretory immunoglobulin A (SIgA), C-reactive protein (CRP), tumor necrosis factor (TNF)-α, interleukins IL-6, IL-8, IL-1β, as well as cortisol and alpha-amylase. During the bottom phase of the hyperbaric saturation, SIgA, CRP, TNF-α, IL-8 and IL-1β increased significantly, whereas IL-6, cortisol and alpha-amylase were unchanged. All markers returned to pre-dive levels after the divers were decompressed back to surface pressure. We conclude that salivary biomarker analysis may be a feasible approach to stress assessment in offshore saturation diving. The results of our pilot test are consonant with an activation of the sympathetic nervous system related to systemic inflammation during hyperbaric and hyperoxic saturation. |
format | Online Article Text |
id | pubmed-8669759 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86697592021-12-15 Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study Monnoyer, Roxane Lautridou, Jacky Deb, Sanjoy Hjelde, Astrid Eftedal, Ingrid Front Physiol Physiology Health monitoring during offshore saturation diving is complicated due to restricted access to the divers, the desire to keep invasive procedures to a minimum, and limited opportunity for laboratory work onboard dive support vessels (DSV). In this pilot study, we examined whether measuring salivary biomarkrers in samples collected by the divers themselves might be a feasible approach to environmental stress assessment. Nine saturation divers were trained in the passive drool method for saliva collection and proceeded to collect samples at nine time points before, during, and after an offshore commercial saturation diving campaign. Samples collected within the hyperbaric living chambers were decompressed and stored frozen at −20°C onboard the DSV until they were shipped to land for analysis. Passive drool samples were collected without loss and assayed for a selection of salivary biomarkers: secretory immunoglobulin A (SIgA), C-reactive protein (CRP), tumor necrosis factor (TNF)-α, interleukins IL-6, IL-8, IL-1β, as well as cortisol and alpha-amylase. During the bottom phase of the hyperbaric saturation, SIgA, CRP, TNF-α, IL-8 and IL-1β increased significantly, whereas IL-6, cortisol and alpha-amylase were unchanged. All markers returned to pre-dive levels after the divers were decompressed back to surface pressure. We conclude that salivary biomarker analysis may be a feasible approach to stress assessment in offshore saturation diving. The results of our pilot test are consonant with an activation of the sympathetic nervous system related to systemic inflammation during hyperbaric and hyperoxic saturation. Frontiers Media S.A. 2021-11-30 /pmc/articles/PMC8669759/ /pubmed/34916964 http://dx.doi.org/10.3389/fphys.2021.791525 Text en Copyright © 2021 Monnoyer, Lautridou, Deb, Hjelde and Eftedal. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Monnoyer, Roxane Lautridou, Jacky Deb, Sanjoy Hjelde, Astrid Eftedal, Ingrid Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study |
title | Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study |
title_full | Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study |
title_fullStr | Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study |
title_full_unstemmed | Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study |
title_short | Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study |
title_sort | using salivary biomarkers for stress assessment in offshore saturation diving: a pilot study |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669759/ https://www.ncbi.nlm.nih.gov/pubmed/34916964 http://dx.doi.org/10.3389/fphys.2021.791525 |
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