Cargando…

Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study

Health monitoring during offshore saturation diving is complicated due to restricted access to the divers, the desire to keep invasive procedures to a minimum, and limited opportunity for laboratory work onboard dive support vessels (DSV). In this pilot study, we examined whether measuring salivary...

Descripción completa

Detalles Bibliográficos
Autores principales: Monnoyer, Roxane, Lautridou, Jacky, Deb, Sanjoy, Hjelde, Astrid, Eftedal, Ingrid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669759/
https://www.ncbi.nlm.nih.gov/pubmed/34916964
http://dx.doi.org/10.3389/fphys.2021.791525
_version_ 1784614843156791296
author Monnoyer, Roxane
Lautridou, Jacky
Deb, Sanjoy
Hjelde, Astrid
Eftedal, Ingrid
author_facet Monnoyer, Roxane
Lautridou, Jacky
Deb, Sanjoy
Hjelde, Astrid
Eftedal, Ingrid
author_sort Monnoyer, Roxane
collection PubMed
description Health monitoring during offshore saturation diving is complicated due to restricted access to the divers, the desire to keep invasive procedures to a minimum, and limited opportunity for laboratory work onboard dive support vessels (DSV). In this pilot study, we examined whether measuring salivary biomarkrers in samples collected by the divers themselves might be a feasible approach to environmental stress assessment. Nine saturation divers were trained in the passive drool method for saliva collection and proceeded to collect samples at nine time points before, during, and after an offshore commercial saturation diving campaign. Samples collected within the hyperbaric living chambers were decompressed and stored frozen at −20°C onboard the DSV until they were shipped to land for analysis. Passive drool samples were collected without loss and assayed for a selection of salivary biomarkers: secretory immunoglobulin A (SIgA), C-reactive protein (CRP), tumor necrosis factor (TNF)-α, interleukins IL-6, IL-8, IL-1β, as well as cortisol and alpha-amylase. During the bottom phase of the hyperbaric saturation, SIgA, CRP, TNF-α, IL-8 and IL-1β increased significantly, whereas IL-6, cortisol and alpha-amylase were unchanged. All markers returned to pre-dive levels after the divers were decompressed back to surface pressure. We conclude that salivary biomarker analysis may be a feasible approach to stress assessment in offshore saturation diving. The results of our pilot test are consonant with an activation of the sympathetic nervous system related to systemic inflammation during hyperbaric and hyperoxic saturation.
format Online
Article
Text
id pubmed-8669759
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-86697592021-12-15 Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study Monnoyer, Roxane Lautridou, Jacky Deb, Sanjoy Hjelde, Astrid Eftedal, Ingrid Front Physiol Physiology Health monitoring during offshore saturation diving is complicated due to restricted access to the divers, the desire to keep invasive procedures to a minimum, and limited opportunity for laboratory work onboard dive support vessels (DSV). In this pilot study, we examined whether measuring salivary biomarkrers in samples collected by the divers themselves might be a feasible approach to environmental stress assessment. Nine saturation divers were trained in the passive drool method for saliva collection and proceeded to collect samples at nine time points before, during, and after an offshore commercial saturation diving campaign. Samples collected within the hyperbaric living chambers were decompressed and stored frozen at −20°C onboard the DSV until they were shipped to land for analysis. Passive drool samples were collected without loss and assayed for a selection of salivary biomarkers: secretory immunoglobulin A (SIgA), C-reactive protein (CRP), tumor necrosis factor (TNF)-α, interleukins IL-6, IL-8, IL-1β, as well as cortisol and alpha-amylase. During the bottom phase of the hyperbaric saturation, SIgA, CRP, TNF-α, IL-8 and IL-1β increased significantly, whereas IL-6, cortisol and alpha-amylase were unchanged. All markers returned to pre-dive levels after the divers were decompressed back to surface pressure. We conclude that salivary biomarker analysis may be a feasible approach to stress assessment in offshore saturation diving. The results of our pilot test are consonant with an activation of the sympathetic nervous system related to systemic inflammation during hyperbaric and hyperoxic saturation. Frontiers Media S.A. 2021-11-30 /pmc/articles/PMC8669759/ /pubmed/34916964 http://dx.doi.org/10.3389/fphys.2021.791525 Text en Copyright © 2021 Monnoyer, Lautridou, Deb, Hjelde and Eftedal. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Monnoyer, Roxane
Lautridou, Jacky
Deb, Sanjoy
Hjelde, Astrid
Eftedal, Ingrid
Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study
title Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study
title_full Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study
title_fullStr Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study
title_full_unstemmed Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study
title_short Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study
title_sort using salivary biomarkers for stress assessment in offshore saturation diving: a pilot study
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669759/
https://www.ncbi.nlm.nih.gov/pubmed/34916964
http://dx.doi.org/10.3389/fphys.2021.791525
work_keys_str_mv AT monnoyerroxane usingsalivarybiomarkersforstressassessmentinoffshoresaturationdivingapilotstudy
AT lautridoujacky usingsalivarybiomarkersforstressassessmentinoffshoresaturationdivingapilotstudy
AT debsanjoy usingsalivarybiomarkersforstressassessmentinoffshoresaturationdivingapilotstudy
AT hjeldeastrid usingsalivarybiomarkersforstressassessmentinoffshoresaturationdivingapilotstudy
AT eftedalingrid usingsalivarybiomarkersforstressassessmentinoffshoresaturationdivingapilotstudy