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m(6)A Regulator-Mediated Methylation Modification Patterns and Characteristics of Immunity in Blood Leukocytes of COVID-19 Patients

Both RNA N6-methyladenosine (m(6)A) modification of SARS-CoV-2 and immune characteristics of the human body have been reported to play an important role in COVID-19, but how the m(6)A methylation modification of leukocytes responds to the virus infection remains unknown. Based on the RNA-seq of 126...

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Autores principales: Qiu, Xiangmin, Hua, Xiaoliang, Li, Qianyin, Zhou, Qin, Chen, Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669770/
https://www.ncbi.nlm.nih.gov/pubmed/34917088
http://dx.doi.org/10.3389/fimmu.2021.774776
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author Qiu, Xiangmin
Hua, Xiaoliang
Li, Qianyin
Zhou, Qin
Chen, Juan
author_facet Qiu, Xiangmin
Hua, Xiaoliang
Li, Qianyin
Zhou, Qin
Chen, Juan
author_sort Qiu, Xiangmin
collection PubMed
description Both RNA N6-methyladenosine (m(6)A) modification of SARS-CoV-2 and immune characteristics of the human body have been reported to play an important role in COVID-19, but how the m(6)A methylation modification of leukocytes responds to the virus infection remains unknown. Based on the RNA-seq of 126 samples from the GEO database, we disclosed that there is a remarkably higher m(6)A modification level of blood leukocytes in patients with COVID-19 compared to patients without COVID-19, and this difference was related to CD4(+) T cells. Two clusters were identified by unsupervised clustering, m(6)A cluster A characterized by T cell activation had a higher prognosis than m(6)A cluster B. Elevated metabolism level, blockage of the immune checkpoint, and lower level of m6A score were observed in m(6)A cluster B. A protective model was constructed based on nine selected genes and it exhibited an excellent predictive value in COVID-19. Further analysis revealed that the protective score was positively correlated to HFD45 and ventilator-free days, while negatively correlated to SOFA score, APACHE-II score, and crp. Our works systematically depicted a complicated correlation between m(6)A methylation modification and host lymphocytes in patients infected with SARS-CoV-2 and provided a well-performing model to predict the patients’ outcomes.
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spelling pubmed-86697702021-12-15 m(6)A Regulator-Mediated Methylation Modification Patterns and Characteristics of Immunity in Blood Leukocytes of COVID-19 Patients Qiu, Xiangmin Hua, Xiaoliang Li, Qianyin Zhou, Qin Chen, Juan Front Immunol Immunology Both RNA N6-methyladenosine (m(6)A) modification of SARS-CoV-2 and immune characteristics of the human body have been reported to play an important role in COVID-19, but how the m(6)A methylation modification of leukocytes responds to the virus infection remains unknown. Based on the RNA-seq of 126 samples from the GEO database, we disclosed that there is a remarkably higher m(6)A modification level of blood leukocytes in patients with COVID-19 compared to patients without COVID-19, and this difference was related to CD4(+) T cells. Two clusters were identified by unsupervised clustering, m(6)A cluster A characterized by T cell activation had a higher prognosis than m(6)A cluster B. Elevated metabolism level, blockage of the immune checkpoint, and lower level of m6A score were observed in m(6)A cluster B. A protective model was constructed based on nine selected genes and it exhibited an excellent predictive value in COVID-19. Further analysis revealed that the protective score was positively correlated to HFD45 and ventilator-free days, while negatively correlated to SOFA score, APACHE-II score, and crp. Our works systematically depicted a complicated correlation between m(6)A methylation modification and host lymphocytes in patients infected with SARS-CoV-2 and provided a well-performing model to predict the patients’ outcomes. Frontiers Media S.A. 2021-11-30 /pmc/articles/PMC8669770/ /pubmed/34917088 http://dx.doi.org/10.3389/fimmu.2021.774776 Text en Copyright © 2021 Qiu, Hua, Li, Zhou and Chen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Qiu, Xiangmin
Hua, Xiaoliang
Li, Qianyin
Zhou, Qin
Chen, Juan
m(6)A Regulator-Mediated Methylation Modification Patterns and Characteristics of Immunity in Blood Leukocytes of COVID-19 Patients
title m(6)A Regulator-Mediated Methylation Modification Patterns and Characteristics of Immunity in Blood Leukocytes of COVID-19 Patients
title_full m(6)A Regulator-Mediated Methylation Modification Patterns and Characteristics of Immunity in Blood Leukocytes of COVID-19 Patients
title_fullStr m(6)A Regulator-Mediated Methylation Modification Patterns and Characteristics of Immunity in Blood Leukocytes of COVID-19 Patients
title_full_unstemmed m(6)A Regulator-Mediated Methylation Modification Patterns and Characteristics of Immunity in Blood Leukocytes of COVID-19 Patients
title_short m(6)A Regulator-Mediated Methylation Modification Patterns and Characteristics of Immunity in Blood Leukocytes of COVID-19 Patients
title_sort m(6)a regulator-mediated methylation modification patterns and characteristics of immunity in blood leukocytes of covid-19 patients
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669770/
https://www.ncbi.nlm.nih.gov/pubmed/34917088
http://dx.doi.org/10.3389/fimmu.2021.774776
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