Loop-Mediated Isothermal Amplification as a Promising Method for Mass COVID-19 Diagnostics

Real-time reverse-transcription polymerase chain reaction (RT-PCR) is currently the most popular method for early COVID-19 diagnostics. However, loop-mediated isothermal amplification (LAMP) is superior to real-time RT-PCR in rapidity and simplicity, since it does not require expensive laboratory eq...

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Detalles Bibliográficos
Autores principales: Makarova, J. A., Fomicheva, K. A., Osipyants, A. I., Shkurnikov, M. Yu., Pokryshchenko, A. A., Tonevitsky, E. A., Vechorko, V. I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pleiades Publishing 2021
Materias:
Problems and Prospects
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670616/
https://www.ncbi.nlm.nih.gov/pubmed/34924586
http://dx.doi.org/10.1134/S0003683821080032
Descripción
Sumario:Real-time reverse-transcription polymerase chain reaction (RT-PCR) is currently the most popular method for early COVID-19 diagnostics. However, loop-mediated isothermal amplification (LAMP) is superior to real-time RT-PCR in rapidity and simplicity, since it does not require expensive laboratory equipment and trained personnel. LAMP-based diagnostic kits for COVID-19 testing already exist, but corresponding tests are not yet widely available. The method has great potential for mass application. Here, we discuss the technical and methodological aspects of its widespread adoption.

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