Loop-Mediated Isothermal Amplification as a Promising Method for Mass COVID-19 Diagnostics

Real-time reverse-transcription polymerase chain reaction (RT-PCR) is currently the most popular method for early COVID-19 diagnostics. However, loop-mediated isothermal amplification (LAMP) is superior to real-time RT-PCR in rapidity and simplicity, since it does not require expensive laboratory eq...

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Autores principales: Makarova, J. A., Fomicheva, K. A., Osipyants, A. I., Shkurnikov, M. Yu., Pokryshchenko, A. A., Tonevitsky, E. A., Vechorko, V. I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pleiades Publishing 2021
Materias:
Problems and Prospects
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670616/
https://www.ncbi.nlm.nih.gov/pubmed/34924586
http://dx.doi.org/10.1134/S0003683821080032
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author Makarova, J. A.
Fomicheva, K. A.
Osipyants, A. I.
Shkurnikov, M. Yu.
Pokryshchenko, A. A.
Tonevitsky, E. A.
Vechorko, V. I.
author_facet Makarova, J. A.
Fomicheva, K. A.
Osipyants, A. I.
Shkurnikov, M. Yu.
Pokryshchenko, A. A.
Tonevitsky, E. A.
Vechorko, V. I.
author_sort Makarova, J. A.
collection PubMed
description Real-time reverse-transcription polymerase chain reaction (RT-PCR) is currently the most popular method for early COVID-19 diagnostics. However, loop-mediated isothermal amplification (LAMP) is superior to real-time RT-PCR in rapidity and simplicity, since it does not require expensive laboratory equipment and trained personnel. LAMP-based diagnostic kits for COVID-19 testing already exist, but corresponding tests are not yet widely available. The method has great potential for mass application. Here, we discuss the technical and methodological aspects of its widespread adoption.
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spelling pubmed-86706162021-12-14 Loop-Mediated Isothermal Amplification as a Promising Method for Mass COVID-19 Diagnostics Makarova, J. A. Fomicheva, K. A. Osipyants, A. I. Shkurnikov, M. Yu. Pokryshchenko, A. A. Tonevitsky, E. A. Vechorko, V. I. Appl Biochem Microbiol Problems and Prospects Real-time reverse-transcription polymerase chain reaction (RT-PCR) is currently the most popular method for early COVID-19 diagnostics. However, loop-mediated isothermal amplification (LAMP) is superior to real-time RT-PCR in rapidity and simplicity, since it does not require expensive laboratory equipment and trained personnel. LAMP-based diagnostic kits for COVID-19 testing already exist, but corresponding tests are not yet widely available. The method has great potential for mass application. Here, we discuss the technical and methodological aspects of its widespread adoption. Pleiades Publishing 2021-12-14 2021 /pmc/articles/PMC8670616/ /pubmed/34924586 http://dx.doi.org/10.1134/S0003683821080032 Text en © Pleiades Publishing, Inc. 2021, ISSN 0003-6838, Applied Biochemistry and Microbiology, 2021, Vol. 57, No. 8, pp. 845–850. © Pleiades Publishing, Inc., 2021.Russian Text © The Author(s), 2020, published in Biotekhnologiya, 2020, Vol. 36, No. 5, pp. 6–12. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Problems and Prospects
Makarova, J. A.
Fomicheva, K. A.
Osipyants, A. I.
Shkurnikov, M. Yu.
Pokryshchenko, A. A.
Tonevitsky, E. A.
Vechorko, V. I.
Loop-Mediated Isothermal Amplification as a Promising Method for Mass COVID-19 Diagnostics
title Loop-Mediated Isothermal Amplification as a Promising Method for Mass COVID-19 Diagnostics
title_full Loop-Mediated Isothermal Amplification as a Promising Method for Mass COVID-19 Diagnostics
title_fullStr Loop-Mediated Isothermal Amplification as a Promising Method for Mass COVID-19 Diagnostics
title_full_unstemmed Loop-Mediated Isothermal Amplification as a Promising Method for Mass COVID-19 Diagnostics
title_short Loop-Mediated Isothermal Amplification as a Promising Method for Mass COVID-19 Diagnostics
title_sort loop-mediated isothermal amplification as a promising method for mass covid-19 diagnostics
topic Problems and Prospects
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670616/
https://www.ncbi.nlm.nih.gov/pubmed/34924586
http://dx.doi.org/10.1134/S0003683821080032
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