Cargando…

Evaluation of the efficacy of LAMP-based SARS-CoV-2 detection with simple RNA extraction from nasopharyngeal swabs: A prospective observational study

The Loopamp SARS-CoV-2 Detection Kit is used for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Loop-mediated isothermal amplification (LAMP) is based on a measurement principle that can be used with a relatively simple device. Detection using this kit requires viral...

Descripción completa

Detalles Bibliográficos
Autores principales: Karino, Masaki, Harada, Mizuki, Yamada, Chihiro, Fukuoka, Kyoko, Sugo, Megumi, Hanada, Hiroyuki, Masuda, Daisaku, Adachi, Shingo, Nakao, Shota, Seki, Masayuki, Yamato, Masaya, Yamashita, Shizuya, Takano, Toru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670695/
https://www.ncbi.nlm.nih.gov/pubmed/34905576
http://dx.doi.org/10.1371/journal.pone.0260732
_version_ 1784615016980283392
author Karino, Masaki
Harada, Mizuki
Yamada, Chihiro
Fukuoka, Kyoko
Sugo, Megumi
Hanada, Hiroyuki
Masuda, Daisaku
Adachi, Shingo
Nakao, Shota
Seki, Masayuki
Yamato, Masaya
Yamashita, Shizuya
Takano, Toru
author_facet Karino, Masaki
Harada, Mizuki
Yamada, Chihiro
Fukuoka, Kyoko
Sugo, Megumi
Hanada, Hiroyuki
Masuda, Daisaku
Adachi, Shingo
Nakao, Shota
Seki, Masayuki
Yamato, Masaya
Yamashita, Shizuya
Takano, Toru
author_sort Karino, Masaki
collection PubMed
description The Loopamp SARS-CoV-2 Detection Kit is used for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Loop-mediated isothermal amplification (LAMP) is based on a measurement principle that can be used with a relatively simple device. Detection using this kit requires viral RNA extraction from samples with the QIAGEN QIAamp Viral Mini Kit (QIAGEN extraction) or the Loopamp Viral RNA Extraction Kit (Eiken extraction), which are recommended by the manufacturer. However, the efficacy of LAMP-based SARS-CoV-2 detection using these extraction methods has not been compared. In this study, we aimed to compare the results of genome extraction and detection from nasopharyngeal swab samples using the QIAGEN and Eiken extraction kits. The present study involved patients who presented to the Rinku General Medical Center with suspected COVID-19 (25 positive and 26 negative cases). A comparison of the results obtained using each extraction method with those obtained via PCR showed that the positive, negative, and overall concordance rates between QIAGEN extraction and PCR were 96.0% (24/25 samples), 100% (26/26), and 98.0% (50/51; κ = 0.96, 95% CI = 0.69–1.00), respectively. Results with Eiken extraction were also favorable, with positive, negative, and overall concordance rates of 88.0% (22/25), 100% (26/26), and 94.1% (48/51; κ = 0.88, 95% CI = 0.61–1.00), respectively. Favorable results were obtained using both QIAGEN and Eiken extraction kits. Since Eiken extraction can be completed in a few minutes, it enables prompt and reliable testing for SARS-CoV-2 detection.
format Online
Article
Text
id pubmed-8670695
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-86706952021-12-15 Evaluation of the efficacy of LAMP-based SARS-CoV-2 detection with simple RNA extraction from nasopharyngeal swabs: A prospective observational study Karino, Masaki Harada, Mizuki Yamada, Chihiro Fukuoka, Kyoko Sugo, Megumi Hanada, Hiroyuki Masuda, Daisaku Adachi, Shingo Nakao, Shota Seki, Masayuki Yamato, Masaya Yamashita, Shizuya Takano, Toru PLoS One Research Article The Loopamp SARS-CoV-2 Detection Kit is used for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Loop-mediated isothermal amplification (LAMP) is based on a measurement principle that can be used with a relatively simple device. Detection using this kit requires viral RNA extraction from samples with the QIAGEN QIAamp Viral Mini Kit (QIAGEN extraction) or the Loopamp Viral RNA Extraction Kit (Eiken extraction), which are recommended by the manufacturer. However, the efficacy of LAMP-based SARS-CoV-2 detection using these extraction methods has not been compared. In this study, we aimed to compare the results of genome extraction and detection from nasopharyngeal swab samples using the QIAGEN and Eiken extraction kits. The present study involved patients who presented to the Rinku General Medical Center with suspected COVID-19 (25 positive and 26 negative cases). A comparison of the results obtained using each extraction method with those obtained via PCR showed that the positive, negative, and overall concordance rates between QIAGEN extraction and PCR were 96.0% (24/25 samples), 100% (26/26), and 98.0% (50/51; κ = 0.96, 95% CI = 0.69–1.00), respectively. Results with Eiken extraction were also favorable, with positive, negative, and overall concordance rates of 88.0% (22/25), 100% (26/26), and 94.1% (48/51; κ = 0.88, 95% CI = 0.61–1.00), respectively. Favorable results were obtained using both QIAGEN and Eiken extraction kits. Since Eiken extraction can be completed in a few minutes, it enables prompt and reliable testing for SARS-CoV-2 detection. Public Library of Science 2021-12-14 /pmc/articles/PMC8670695/ /pubmed/34905576 http://dx.doi.org/10.1371/journal.pone.0260732 Text en © 2021 Karino et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Karino, Masaki
Harada, Mizuki
Yamada, Chihiro
Fukuoka, Kyoko
Sugo, Megumi
Hanada, Hiroyuki
Masuda, Daisaku
Adachi, Shingo
Nakao, Shota
Seki, Masayuki
Yamato, Masaya
Yamashita, Shizuya
Takano, Toru
Evaluation of the efficacy of LAMP-based SARS-CoV-2 detection with simple RNA extraction from nasopharyngeal swabs: A prospective observational study
title Evaluation of the efficacy of LAMP-based SARS-CoV-2 detection with simple RNA extraction from nasopharyngeal swabs: A prospective observational study
title_full Evaluation of the efficacy of LAMP-based SARS-CoV-2 detection with simple RNA extraction from nasopharyngeal swabs: A prospective observational study
title_fullStr Evaluation of the efficacy of LAMP-based SARS-CoV-2 detection with simple RNA extraction from nasopharyngeal swabs: A prospective observational study
title_full_unstemmed Evaluation of the efficacy of LAMP-based SARS-CoV-2 detection with simple RNA extraction from nasopharyngeal swabs: A prospective observational study
title_short Evaluation of the efficacy of LAMP-based SARS-CoV-2 detection with simple RNA extraction from nasopharyngeal swabs: A prospective observational study
title_sort evaluation of the efficacy of lamp-based sars-cov-2 detection with simple rna extraction from nasopharyngeal swabs: a prospective observational study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670695/
https://www.ncbi.nlm.nih.gov/pubmed/34905576
http://dx.doi.org/10.1371/journal.pone.0260732
work_keys_str_mv AT karinomasaki evaluationoftheefficacyoflampbasedsarscov2detectionwithsimplernaextractionfromnasopharyngealswabsaprospectiveobservationalstudy
AT haradamizuki evaluationoftheefficacyoflampbasedsarscov2detectionwithsimplernaextractionfromnasopharyngealswabsaprospectiveobservationalstudy
AT yamadachihiro evaluationoftheefficacyoflampbasedsarscov2detectionwithsimplernaextractionfromnasopharyngealswabsaprospectiveobservationalstudy
AT fukuokakyoko evaluationoftheefficacyoflampbasedsarscov2detectionwithsimplernaextractionfromnasopharyngealswabsaprospectiveobservationalstudy
AT sugomegumi evaluationoftheefficacyoflampbasedsarscov2detectionwithsimplernaextractionfromnasopharyngealswabsaprospectiveobservationalstudy
AT hanadahiroyuki evaluationoftheefficacyoflampbasedsarscov2detectionwithsimplernaextractionfromnasopharyngealswabsaprospectiveobservationalstudy
AT masudadaisaku evaluationoftheefficacyoflampbasedsarscov2detectionwithsimplernaextractionfromnasopharyngealswabsaprospectiveobservationalstudy
AT adachishingo evaluationoftheefficacyoflampbasedsarscov2detectionwithsimplernaextractionfromnasopharyngealswabsaprospectiveobservationalstudy
AT nakaoshota evaluationoftheefficacyoflampbasedsarscov2detectionwithsimplernaextractionfromnasopharyngealswabsaprospectiveobservationalstudy
AT sekimasayuki evaluationoftheefficacyoflampbasedsarscov2detectionwithsimplernaextractionfromnasopharyngealswabsaprospectiveobservationalstudy
AT yamatomasaya evaluationoftheefficacyoflampbasedsarscov2detectionwithsimplernaextractionfromnasopharyngealswabsaprospectiveobservationalstudy
AT yamashitashizuya evaluationoftheefficacyoflampbasedsarscov2detectionwithsimplernaextractionfromnasopharyngealswabsaprospectiveobservationalstudy
AT takanotoru evaluationoftheefficacyoflampbasedsarscov2detectionwithsimplernaextractionfromnasopharyngealswabsaprospectiveobservationalstudy