Streptomyces tsukubensis VKM Aс-2618D—an Effective Producer of Tacrolimus

The Streptomyces sp. VKM Ac-2618D strain has been identified, and its morphological and physiological features have been studied in relation to the production of the immunosuppressant tacrolimus. The phenotypic variability of the strain was analyzed, and a dissociant with a high level of tacrolimus...

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Autores principales: Poshekhontseva, V. Yu., Fokina, V. V., Tarlachkov, S. V., Machulin, A. V., Shutov, A. A., Donova, M. V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pleiades Publishing 2021
Materias:
Producers, Biology, Selection, and Genetic Engineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670718/
https://www.ncbi.nlm.nih.gov/pubmed/34924587
http://dx.doi.org/10.1134/S0003683821090064
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author Poshekhontseva, V. Yu.
Fokina, V. V.
Tarlachkov, S. V.
Machulin, A. V.
Shutov, A. A.
Donova, M. V.
author_facet Poshekhontseva, V. Yu.
Fokina, V. V.
Tarlachkov, S. V.
Machulin, A. V.
Shutov, A. A.
Donova, M. V.
author_sort Poshekhontseva, V. Yu.
collection PubMed
description The Streptomyces sp. VKM Ac-2618D strain has been identified, and its morphological and physiological features have been studied in relation to the production of the immunosuppressant tacrolimus. The phenotypic variability of the strain was analyzed, and a dissociant with a high level of tacrolimus production was selected. Based on a comprehensive study of morphological, physiological, and chemotaxonomic properties and on phylogenetic analysis, the strain was named Streptomyces tsukubensis VKM Ac-2618D. The strain genome contains the full version of the tacrolimus biosynthetic gene cluster. The advantages of fed-batch cultivation mode for tacrolimus biosynthesis are shown. The results broaden the understanding of the characteristics of polyketide biosynthesis and can be used in the development of technology for tacrolimus production.
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spelling pubmed-86707182021-12-15 Streptomyces tsukubensis VKM Aс-2618D—an Effective Producer of Tacrolimus Poshekhontseva, V. Yu. Fokina, V. V. Tarlachkov, S. V. Machulin, A. V. Shutov, A. A. Donova, M. V. Appl Biochem Microbiol Producers, Biology, Selection, and Genetic Engineering The Streptomyces sp. VKM Ac-2618D strain has been identified, and its morphological and physiological features have been studied in relation to the production of the immunosuppressant tacrolimus. The phenotypic variability of the strain was analyzed, and a dissociant with a high level of tacrolimus production was selected. Based on a comprehensive study of morphological, physiological, and chemotaxonomic properties and on phylogenetic analysis, the strain was named Streptomyces tsukubensis VKM Ac-2618D. The strain genome contains the full version of the tacrolimus biosynthetic gene cluster. The advantages of fed-batch cultivation mode for tacrolimus biosynthesis are shown. The results broaden the understanding of the characteristics of polyketide biosynthesis and can be used in the development of technology for tacrolimus production. Pleiades Publishing 2021-12-14 2021 /pmc/articles/PMC8670718/ /pubmed/34924587 http://dx.doi.org/10.1134/S0003683821090064 Text en © Pleiades Publishing, Inc. 2021, ISSN 0003-6838, Applied Biochemistry and Microbiology, 2021, Vol. 57, No. 9, pp. 939–948. © Pleiades Publishing, Inc., 2021.Russian Text © The Author(s), 2021, published in Biotekhnologiya, 2021, Vol. 37, No. 1, pp. 26–36. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Producers, Biology, Selection, and Genetic Engineering
Poshekhontseva, V. Yu.
Fokina, V. V.
Tarlachkov, S. V.
Machulin, A. V.
Shutov, A. A.
Donova, M. V.
Streptomyces tsukubensis VKM Aс-2618D—an Effective Producer of Tacrolimus
title Streptomyces tsukubensis VKM Aс-2618D—an Effective Producer of Tacrolimus
title_full Streptomyces tsukubensis VKM Aс-2618D—an Effective Producer of Tacrolimus
title_fullStr Streptomyces tsukubensis VKM Aс-2618D—an Effective Producer of Tacrolimus
title_full_unstemmed Streptomyces tsukubensis VKM Aс-2618D—an Effective Producer of Tacrolimus
title_short Streptomyces tsukubensis VKM Aс-2618D—an Effective Producer of Tacrolimus
title_sort streptomyces tsukubensis vkm aс-2618d—an effective producer of tacrolimus
topic Producers, Biology, Selection, and Genetic Engineering
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670718/
https://www.ncbi.nlm.nih.gov/pubmed/34924587
http://dx.doi.org/10.1134/S0003683821090064
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