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An MD-2-related lipid-recognition protein is required for insect reproduction and integument development

The myeloid differentiation factor 2 (MD-2)-related lipid-recognition protein is involved in immune responses through recognizing bacteria lipopolysaccharide in mammals, arthropods and plants. However, the physiological roles of MD-2 in other biological processes are largely unknown. Here, we identi...

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Autores principales: Wang, Wei, Ma, Ya, Yang, Rui-Rui, Cheng, Xu, Huang, Hai-Jian, Zhang, Chuan-Xi, Bao, Yan-Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670961/
https://www.ncbi.nlm.nih.gov/pubmed/34905699
http://dx.doi.org/10.1098/rsob.210170
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author Wang, Wei
Ma, Ya
Yang, Rui-Rui
Cheng, Xu
Huang, Hai-Jian
Zhang, Chuan-Xi
Bao, Yan-Yuan
author_facet Wang, Wei
Ma, Ya
Yang, Rui-Rui
Cheng, Xu
Huang, Hai-Jian
Zhang, Chuan-Xi
Bao, Yan-Yuan
author_sort Wang, Wei
collection PubMed
description The myeloid differentiation factor 2 (MD-2)-related lipid-recognition protein is involved in immune responses through recognizing bacteria lipopolysaccharide in mammals, arthropods and plants. However, the physiological roles of MD-2 in other biological processes are largely unknown. Here, we identified three homologue MD-2 genes (NlML1, NlML2 and NlML3) by searching the genome and transcriptome databases of the brown planthopper Nilaparvata lugens, a hemipteran insect species. Temporospatial analysis showed that the NlML1 gene was highly expressed in the fat body but much less so in the other tissues, while the NlML2 and NlML3 genes were highly expressed in the testis or digestive tract. RNA interference-mediated depletion of the NlML1 gene significantly downregulated the transcription of numerous integument protein genes. The NlML1 knockdown led to moulting failure and mortality at the nymph–adult transition phase, impaired egg laying and hatching, and reduced 20-hydroxyecdysone (20E) production in the nymphs. 20E could rescue the deficient moulting phenotypes derived from dsNlML1 RNAi. These novel findings indicate that NlML1 is required for nymphal moulting and female reproductive success as it plays an important role in regulating 20E synthesis, lipid and chitin metabolisms in N. lugens, thus contributing to our understanding of developmental and reproductive mechanisms in insects.
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spelling pubmed-86709612021-12-28 An MD-2-related lipid-recognition protein is required for insect reproduction and integument development Wang, Wei Ma, Ya Yang, Rui-Rui Cheng, Xu Huang, Hai-Jian Zhang, Chuan-Xi Bao, Yan-Yuan Open Biol Research The myeloid differentiation factor 2 (MD-2)-related lipid-recognition protein is involved in immune responses through recognizing bacteria lipopolysaccharide in mammals, arthropods and plants. However, the physiological roles of MD-2 in other biological processes are largely unknown. Here, we identified three homologue MD-2 genes (NlML1, NlML2 and NlML3) by searching the genome and transcriptome databases of the brown planthopper Nilaparvata lugens, a hemipteran insect species. Temporospatial analysis showed that the NlML1 gene was highly expressed in the fat body but much less so in the other tissues, while the NlML2 and NlML3 genes were highly expressed in the testis or digestive tract. RNA interference-mediated depletion of the NlML1 gene significantly downregulated the transcription of numerous integument protein genes. The NlML1 knockdown led to moulting failure and mortality at the nymph–adult transition phase, impaired egg laying and hatching, and reduced 20-hydroxyecdysone (20E) production in the nymphs. 20E could rescue the deficient moulting phenotypes derived from dsNlML1 RNAi. These novel findings indicate that NlML1 is required for nymphal moulting and female reproductive success as it plays an important role in regulating 20E synthesis, lipid and chitin metabolisms in N. lugens, thus contributing to our understanding of developmental and reproductive mechanisms in insects. The Royal Society 2021-12-15 /pmc/articles/PMC8670961/ /pubmed/34905699 http://dx.doi.org/10.1098/rsob.210170 Text en © 2021 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited.
spellingShingle Research
Wang, Wei
Ma, Ya
Yang, Rui-Rui
Cheng, Xu
Huang, Hai-Jian
Zhang, Chuan-Xi
Bao, Yan-Yuan
An MD-2-related lipid-recognition protein is required for insect reproduction and integument development
title An MD-2-related lipid-recognition protein is required for insect reproduction and integument development
title_full An MD-2-related lipid-recognition protein is required for insect reproduction and integument development
title_fullStr An MD-2-related lipid-recognition protein is required for insect reproduction and integument development
title_full_unstemmed An MD-2-related lipid-recognition protein is required for insect reproduction and integument development
title_short An MD-2-related lipid-recognition protein is required for insect reproduction and integument development
title_sort md-2-related lipid-recognition protein is required for insect reproduction and integument development
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670961/
https://www.ncbi.nlm.nih.gov/pubmed/34905699
http://dx.doi.org/10.1098/rsob.210170
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