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Micro-computed tomography: A novel diagnostic technique for the evaluation of gastrointestinal specimens

Micro-computed tomography (micro-CT) is a non-destructive modality that can be used to obtain high-resolution three-dimensional (3 D) images of the whole sample tissue; the usefulness of micro-CT has been reported for evaluation of breast cancer and lung cancer. However, this novel diagnostic techni...

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Autores principales: Kawata, Noboru, Teplov, Alexei, Ntiamoah, Peter, Shia, Jinru, Hameed, Meera, Yagi, Yukako
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Georg Thieme Verlag KG 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670991/
https://www.ncbi.nlm.nih.gov/pubmed/34917457
http://dx.doi.org/10.1055/a-1546-8063
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author Kawata, Noboru
Teplov, Alexei
Ntiamoah, Peter
Shia, Jinru
Hameed, Meera
Yagi, Yukako
author_facet Kawata, Noboru
Teplov, Alexei
Ntiamoah, Peter
Shia, Jinru
Hameed, Meera
Yagi, Yukako
author_sort Kawata, Noboru
collection PubMed
description Micro-computed tomography (micro-CT) is a non-destructive modality that can be used to obtain high-resolution three-dimensional (3 D) images of the whole sample tissue; the usefulness of micro-CT has been reported for evaluation of breast cancer and lung cancer. However, this novel diagnostic technique has never been used for evaluating endoscopically resected gastrointestinal specimens. In the present study, we scanned 13 formalin-fixed paraffin-embedded (FFPE) tissue blocks of a normal human colon and gastric tissue samples using micro-CT. The evaluation comprised a comparison of the acquired whole block images with the images of the corresponding cross-sectional slice of the hematoxylin and eosin-stained slide. Micro-CT was able to produce images of the whole sample and clearly depict tissues such as glandular structures, muscularis mucosae, and blood vessels in the FFPE tissue blocks of normal gastrointestinal samples. Furthermore, the 3 D reconstructed could be used to create a cross-sectional image and reflected the surface structure of samples obtained from any site. Micro-CT has the potential to become a highly promising pathological diagnostic assistance tool for endoscopically resected gastrointestinal specimens in combination with conventional microscopic examination.
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spelling pubmed-86709912021-12-15 Micro-computed tomography: A novel diagnostic technique for the evaluation of gastrointestinal specimens Kawata, Noboru Teplov, Alexei Ntiamoah, Peter Shia, Jinru Hameed, Meera Yagi, Yukako Endosc Int Open Micro-computed tomography (micro-CT) is a non-destructive modality that can be used to obtain high-resolution three-dimensional (3 D) images of the whole sample tissue; the usefulness of micro-CT has been reported for evaluation of breast cancer and lung cancer. However, this novel diagnostic technique has never been used for evaluating endoscopically resected gastrointestinal specimens. In the present study, we scanned 13 formalin-fixed paraffin-embedded (FFPE) tissue blocks of a normal human colon and gastric tissue samples using micro-CT. The evaluation comprised a comparison of the acquired whole block images with the images of the corresponding cross-sectional slice of the hematoxylin and eosin-stained slide. Micro-CT was able to produce images of the whole sample and clearly depict tissues such as glandular structures, muscularis mucosae, and blood vessels in the FFPE tissue blocks of normal gastrointestinal samples. Furthermore, the 3 D reconstructed could be used to create a cross-sectional image and reflected the surface structure of samples obtained from any site. Micro-CT has the potential to become a highly promising pathological diagnostic assistance tool for endoscopically resected gastrointestinal specimens in combination with conventional microscopic examination. Georg Thieme Verlag KG 2021-12-14 /pmc/articles/PMC8670991/ /pubmed/34917457 http://dx.doi.org/10.1055/a-1546-8063 Text en The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited.
spellingShingle Kawata, Noboru
Teplov, Alexei
Ntiamoah, Peter
Shia, Jinru
Hameed, Meera
Yagi, Yukako
Micro-computed tomography: A novel diagnostic technique for the evaluation of gastrointestinal specimens
title Micro-computed tomography: A novel diagnostic technique for the evaluation of gastrointestinal specimens
title_full Micro-computed tomography: A novel diagnostic technique for the evaluation of gastrointestinal specimens
title_fullStr Micro-computed tomography: A novel diagnostic technique for the evaluation of gastrointestinal specimens
title_full_unstemmed Micro-computed tomography: A novel diagnostic technique for the evaluation of gastrointestinal specimens
title_short Micro-computed tomography: A novel diagnostic technique for the evaluation of gastrointestinal specimens
title_sort micro-computed tomography: a novel diagnostic technique for the evaluation of gastrointestinal specimens
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670991/
https://www.ncbi.nlm.nih.gov/pubmed/34917457
http://dx.doi.org/10.1055/a-1546-8063
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