Cargando…

An NIR-Triggered Au Nanocage Used for Photo-Thermo Therapy of Chronic Wound in Diabetic Rats Through Bacterial Membrane Destruction and Skin Cell Mitochondrial Protection

Bacterial infection and its severe oxidative stress reaction will cause damage to skin cell mitochondria, resulting in long-lasting wound healing and great pain to patients. Thus, delayed wound healing in diabetic patients with Staphylococcus aureus infection is a principal challenge worldwide. Ther...

Descripción completa

Detalles Bibliográficos
Autores principales: Ding, Jiaxin, Gao, Binbin, Chen, Zhenhua, Mei, Xifan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8671044/
https://www.ncbi.nlm.nih.gov/pubmed/34925036
http://dx.doi.org/10.3389/fphar.2021.779944
_version_ 1784615086308982784
author Ding, Jiaxin
Gao, Binbin
Chen, Zhenhua
Mei, Xifan
author_facet Ding, Jiaxin
Gao, Binbin
Chen, Zhenhua
Mei, Xifan
author_sort Ding, Jiaxin
collection PubMed
description Bacterial infection and its severe oxidative stress reaction will cause damage to skin cell mitochondria, resulting in long-lasting wound healing and great pain to patients. Thus, delayed wound healing in diabetic patients with Staphylococcus aureus infection is a principal challenge worldwide. Therefore, novel biomaterials with multifunction of bacterial membrane destruction and skin cell mitochondrial protection are urgently needed to be developed to address this challenge. In this work, novel gold cage (AuNCs) modified with epigallocatechin gallate (EGCG) were prepared to treat delayed diabetic wounds. The results showed that Au-EGCG had a high and stable photothermal conversion efficiency under near-infrared irradiation, and the scavenging rate of Au-EGCG for S. aureus could reach 95%. The production of large amounts of reactive oxygen species (ROS) leads to the disruption of bacterial membranes, inducing bacterial lysis and apoptosis. Meanwhile, Au-EGCG fused into hydrogel (Au-EGCG@H) promoted the migration and proliferation of human umbilical cord endothelial cells, reduced cellular mitochondrial damage and oxidative stress in the presence of infection, and significantly increased the basic fibroblast growth factor expression and vascular endothelial growth factor. In addition, animal studies showed that wound closure was 97.2% after 12 days of treatment, and the healing of chronic diabetic wounds was significantly accelerated. Au-EGCG nanoplatforms were successfully prepared to promote cell migration and angiogenesis in diabetic rats while removing S. aureus, reducing oxidative stress in cells, and restoring impaired mitochondrial function. Au-EGCG provides an effective, biocompatible, and multifunctional therapeutic strategy for chronic diabetic wounds.
format Online
Article
Text
id pubmed-8671044
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-86710442021-12-16 An NIR-Triggered Au Nanocage Used for Photo-Thermo Therapy of Chronic Wound in Diabetic Rats Through Bacterial Membrane Destruction and Skin Cell Mitochondrial Protection Ding, Jiaxin Gao, Binbin Chen, Zhenhua Mei, Xifan Front Pharmacol Pharmacology Bacterial infection and its severe oxidative stress reaction will cause damage to skin cell mitochondria, resulting in long-lasting wound healing and great pain to patients. Thus, delayed wound healing in diabetic patients with Staphylococcus aureus infection is a principal challenge worldwide. Therefore, novel biomaterials with multifunction of bacterial membrane destruction and skin cell mitochondrial protection are urgently needed to be developed to address this challenge. In this work, novel gold cage (AuNCs) modified with epigallocatechin gallate (EGCG) were prepared to treat delayed diabetic wounds. The results showed that Au-EGCG had a high and stable photothermal conversion efficiency under near-infrared irradiation, and the scavenging rate of Au-EGCG for S. aureus could reach 95%. The production of large amounts of reactive oxygen species (ROS) leads to the disruption of bacterial membranes, inducing bacterial lysis and apoptosis. Meanwhile, Au-EGCG fused into hydrogel (Au-EGCG@H) promoted the migration and proliferation of human umbilical cord endothelial cells, reduced cellular mitochondrial damage and oxidative stress in the presence of infection, and significantly increased the basic fibroblast growth factor expression and vascular endothelial growth factor. In addition, animal studies showed that wound closure was 97.2% after 12 days of treatment, and the healing of chronic diabetic wounds was significantly accelerated. Au-EGCG nanoplatforms were successfully prepared to promote cell migration and angiogenesis in diabetic rats while removing S. aureus, reducing oxidative stress in cells, and restoring impaired mitochondrial function. Au-EGCG provides an effective, biocompatible, and multifunctional therapeutic strategy for chronic diabetic wounds. Frontiers Media S.A. 2021-11-30 /pmc/articles/PMC8671044/ /pubmed/34925036 http://dx.doi.org/10.3389/fphar.2021.779944 Text en Copyright © 2021 Ding, Gao, Chen and Mei. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Ding, Jiaxin
Gao, Binbin
Chen, Zhenhua
Mei, Xifan
An NIR-Triggered Au Nanocage Used for Photo-Thermo Therapy of Chronic Wound in Diabetic Rats Through Bacterial Membrane Destruction and Skin Cell Mitochondrial Protection
title An NIR-Triggered Au Nanocage Used for Photo-Thermo Therapy of Chronic Wound in Diabetic Rats Through Bacterial Membrane Destruction and Skin Cell Mitochondrial Protection
title_full An NIR-Triggered Au Nanocage Used for Photo-Thermo Therapy of Chronic Wound in Diabetic Rats Through Bacterial Membrane Destruction and Skin Cell Mitochondrial Protection
title_fullStr An NIR-Triggered Au Nanocage Used for Photo-Thermo Therapy of Chronic Wound in Diabetic Rats Through Bacterial Membrane Destruction and Skin Cell Mitochondrial Protection
title_full_unstemmed An NIR-Triggered Au Nanocage Used for Photo-Thermo Therapy of Chronic Wound in Diabetic Rats Through Bacterial Membrane Destruction and Skin Cell Mitochondrial Protection
title_short An NIR-Triggered Au Nanocage Used for Photo-Thermo Therapy of Chronic Wound in Diabetic Rats Through Bacterial Membrane Destruction and Skin Cell Mitochondrial Protection
title_sort nir-triggered au nanocage used for photo-thermo therapy of chronic wound in diabetic rats through bacterial membrane destruction and skin cell mitochondrial protection
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8671044/
https://www.ncbi.nlm.nih.gov/pubmed/34925036
http://dx.doi.org/10.3389/fphar.2021.779944
work_keys_str_mv AT dingjiaxin annirtriggeredaunanocageusedforphotothermotherapyofchronicwoundindiabeticratsthroughbacterialmembranedestructionandskincellmitochondrialprotection
AT gaobinbin annirtriggeredaunanocageusedforphotothermotherapyofchronicwoundindiabeticratsthroughbacterialmembranedestructionandskincellmitochondrialprotection
AT chenzhenhua annirtriggeredaunanocageusedforphotothermotherapyofchronicwoundindiabeticratsthroughbacterialmembranedestructionandskincellmitochondrialprotection
AT meixifan annirtriggeredaunanocageusedforphotothermotherapyofchronicwoundindiabeticratsthroughbacterialmembranedestructionandskincellmitochondrialprotection
AT dingjiaxin nirtriggeredaunanocageusedforphotothermotherapyofchronicwoundindiabeticratsthroughbacterialmembranedestructionandskincellmitochondrialprotection
AT gaobinbin nirtriggeredaunanocageusedforphotothermotherapyofchronicwoundindiabeticratsthroughbacterialmembranedestructionandskincellmitochondrialprotection
AT chenzhenhua nirtriggeredaunanocageusedforphotothermotherapyofchronicwoundindiabeticratsthroughbacterialmembranedestructionandskincellmitochondrialprotection
AT meixifan nirtriggeredaunanocageusedforphotothermotherapyofchronicwoundindiabeticratsthroughbacterialmembranedestructionandskincellmitochondrialprotection