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Analysis of cytokines in the aqueous humor during intravitreal Ranibizumab treatment of diabetic macular edema
This study aimed to analyze the concentrations of VEGF, b-FGF, TNF, interleukin (IL)-1, IL-6, IL-8, IL-10, and IL-12 in the aqueous humor of patients with diabetic macular edema with and without peripheral retinal ischemia and to ascertain the changes in the levels of these molecules during treatmen...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8671392/ https://www.ncbi.nlm.nih.gov/pubmed/34907265 http://dx.doi.org/10.1038/s41598-021-03433-2 |
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author | de Freitas, Luiz Guilherme Azevedo Isaac, David Leonardo Cruvinel Abud, Murilo Batista Segundo, Alexandre Dantas Soares Quintas Barros, Mariana Larissa Alvino de Albuquerque, Gabriela Caroline Monteiro Guimarães, Bruna Dantas Aires de Morais, Clarice Neuenschwander Lins de Ávila, Marcos Pereira |
author_facet | de Freitas, Luiz Guilherme Azevedo Isaac, David Leonardo Cruvinel Abud, Murilo Batista Segundo, Alexandre Dantas Soares Quintas Barros, Mariana Larissa Alvino de Albuquerque, Gabriela Caroline Monteiro Guimarães, Bruna Dantas Aires de Morais, Clarice Neuenschwander Lins de Ávila, Marcos Pereira |
author_sort | de Freitas, Luiz Guilherme Azevedo |
collection | PubMed |
description | This study aimed to analyze the concentrations of VEGF, b-FGF, TNF, interleukin (IL)-1, IL-6, IL-8, IL-10, and IL-12 in the aqueous humor of patients with diabetic macular edema with and without peripheral retinal ischemia and to ascertain the changes in the levels of these molecules during treatment with ranibizumab. A therapeutic, prospective, randomized interventional study was carried out. Twenty-four eyes from 24 patients were studied and divided into 3 groups. Group 1 (9 eyes) included patients with diabetic macular edema without peripheral ischemia. Group 2 (10 eyes) included patients with diabetic macular edema with peripheral ischemia. Group 3 (5 eyes), the control group, included patients without systemic and/or eye diseases. Patients in Groups 1 and 2 received 3 intravitreal injections of 2 mg/0.05 ml ranibizumab at an interval of approximately 30 days. Before administering the injections, the aqueous humor was collected. In the control group, aqueous humor was collected before facetectomy. During treatment, the median IL-6 concentration significantly increased in Group 1 but showed a slight but not significant decrease in Group 2. Interleukin 8 levels were significantly different at the end of treatment compared to the beginning in Groups 1 and 2. TNF, IL-1, IL-10, and IL-12 levels were practically unchanged in both groups. VEGF was significantly reduced at the end of the study in Groups 1 and 2. B-FGF was not detected in most of the studied patients, and in those with detectable levels, there was no significant variation. There was a significant increase in the median level of interleukin 6 in the group without ischemia and a significant decrease in VEGF in both groups. The cytokines TNF, IL-1, IL-10, and IL-12 did not show significant variation. |
format | Online Article Text |
id | pubmed-8671392 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-86713922021-12-15 Analysis of cytokines in the aqueous humor during intravitreal Ranibizumab treatment of diabetic macular edema de Freitas, Luiz Guilherme Azevedo Isaac, David Leonardo Cruvinel Abud, Murilo Batista Segundo, Alexandre Dantas Soares Quintas Barros, Mariana Larissa Alvino de Albuquerque, Gabriela Caroline Monteiro Guimarães, Bruna Dantas Aires de Morais, Clarice Neuenschwander Lins de Ávila, Marcos Pereira Sci Rep Article This study aimed to analyze the concentrations of VEGF, b-FGF, TNF, interleukin (IL)-1, IL-6, IL-8, IL-10, and IL-12 in the aqueous humor of patients with diabetic macular edema with and without peripheral retinal ischemia and to ascertain the changes in the levels of these molecules during treatment with ranibizumab. A therapeutic, prospective, randomized interventional study was carried out. Twenty-four eyes from 24 patients were studied and divided into 3 groups. Group 1 (9 eyes) included patients with diabetic macular edema without peripheral ischemia. Group 2 (10 eyes) included patients with diabetic macular edema with peripheral ischemia. Group 3 (5 eyes), the control group, included patients without systemic and/or eye diseases. Patients in Groups 1 and 2 received 3 intravitreal injections of 2 mg/0.05 ml ranibizumab at an interval of approximately 30 days. Before administering the injections, the aqueous humor was collected. In the control group, aqueous humor was collected before facetectomy. During treatment, the median IL-6 concentration significantly increased in Group 1 but showed a slight but not significant decrease in Group 2. Interleukin 8 levels were significantly different at the end of treatment compared to the beginning in Groups 1 and 2. TNF, IL-1, IL-10, and IL-12 levels were practically unchanged in both groups. VEGF was significantly reduced at the end of the study in Groups 1 and 2. B-FGF was not detected in most of the studied patients, and in those with detectable levels, there was no significant variation. There was a significant increase in the median level of interleukin 6 in the group without ischemia and a significant decrease in VEGF in both groups. The cytokines TNF, IL-1, IL-10, and IL-12 did not show significant variation. Nature Publishing Group UK 2021-12-14 /pmc/articles/PMC8671392/ /pubmed/34907265 http://dx.doi.org/10.1038/s41598-021-03433-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article de Freitas, Luiz Guilherme Azevedo Isaac, David Leonardo Cruvinel Abud, Murilo Batista Segundo, Alexandre Dantas Soares Quintas Barros, Mariana Larissa Alvino de Albuquerque, Gabriela Caroline Monteiro Guimarães, Bruna Dantas Aires de Morais, Clarice Neuenschwander Lins de Ávila, Marcos Pereira Analysis of cytokines in the aqueous humor during intravitreal Ranibizumab treatment of diabetic macular edema |
title | Analysis of cytokines in the aqueous humor during intravitreal Ranibizumab treatment of diabetic macular edema |
title_full | Analysis of cytokines in the aqueous humor during intravitreal Ranibizumab treatment of diabetic macular edema |
title_fullStr | Analysis of cytokines in the aqueous humor during intravitreal Ranibizumab treatment of diabetic macular edema |
title_full_unstemmed | Analysis of cytokines in the aqueous humor during intravitreal Ranibizumab treatment of diabetic macular edema |
title_short | Analysis of cytokines in the aqueous humor during intravitreal Ranibizumab treatment of diabetic macular edema |
title_sort | analysis of cytokines in the aqueous humor during intravitreal ranibizumab treatment of diabetic macular edema |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8671392/ https://www.ncbi.nlm.nih.gov/pubmed/34907265 http://dx.doi.org/10.1038/s41598-021-03433-2 |
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