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Mild hyperthermia induced by gold nanorods acts as a dual-edge blade in the fate of SH-SY5Y cells via autophagy

Unraveling unwanted side effects of nanotechnology-based therapies like photothermal therapy (PTT) is vital in translational nanomedicine. Herein, we monitored the relationship between autophagic response at the transcriptional level by using a PCR array and tumor formation ability by colony formati...

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Autores principales: Ghafarkhani, Maryam, Avci, Cigir Biray, Rahbarghazi, Reza, Karimi, Abbas, Sadeghizadeh, Majid, Zarebkohan, Amir, Bani, Farhad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8671444/
https://www.ncbi.nlm.nih.gov/pubmed/34907215
http://dx.doi.org/10.1038/s41598-021-02697-y
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author Ghafarkhani, Maryam
Avci, Cigir Biray
Rahbarghazi, Reza
Karimi, Abbas
Sadeghizadeh, Majid
Zarebkohan, Amir
Bani, Farhad
author_facet Ghafarkhani, Maryam
Avci, Cigir Biray
Rahbarghazi, Reza
Karimi, Abbas
Sadeghizadeh, Majid
Zarebkohan, Amir
Bani, Farhad
author_sort Ghafarkhani, Maryam
collection PubMed
description Unraveling unwanted side effects of nanotechnology-based therapies like photothermal therapy (PTT) is vital in translational nanomedicine. Herein, we monitored the relationship between autophagic response at the transcriptional level by using a PCR array and tumor formation ability by colony formation assay in the human neuroblastoma cell line, SH-SY5Y, 48 h after being exposed to two different mild hyperthermia (43 and 48 °C) induced by PTT. In this regard, the promotion of apoptosis and autophagy were evaluated using immunofluorescence imaging and flow cytometry analyses. Protein levels of Ki-67, P62, and LC3 were measured using ELISA. Our results showed that of 86 genes associated with autophagy, the expression of 54 genes was changed in response to PTT. Also, we showed that chaperone-mediated autophagy (CMA) and macroautophagy are stimulated in PTT. Importantly, the results of this study also showed significant changes in genes related to the crosstalk between autophagy, dormancy, and metastatic activity of treated cells. Our findings illustrated that PTT enhances the aggressiveness of cancer cells at 43 °C, in contrast to 48 °C by the regulation of autophagy-dependent manner.
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spelling pubmed-86714442021-12-16 Mild hyperthermia induced by gold nanorods acts as a dual-edge blade in the fate of SH-SY5Y cells via autophagy Ghafarkhani, Maryam Avci, Cigir Biray Rahbarghazi, Reza Karimi, Abbas Sadeghizadeh, Majid Zarebkohan, Amir Bani, Farhad Sci Rep Article Unraveling unwanted side effects of nanotechnology-based therapies like photothermal therapy (PTT) is vital in translational nanomedicine. Herein, we monitored the relationship between autophagic response at the transcriptional level by using a PCR array and tumor formation ability by colony formation assay in the human neuroblastoma cell line, SH-SY5Y, 48 h after being exposed to two different mild hyperthermia (43 and 48 °C) induced by PTT. In this regard, the promotion of apoptosis and autophagy were evaluated using immunofluorescence imaging and flow cytometry analyses. Protein levels of Ki-67, P62, and LC3 were measured using ELISA. Our results showed that of 86 genes associated with autophagy, the expression of 54 genes was changed in response to PTT. Also, we showed that chaperone-mediated autophagy (CMA) and macroautophagy are stimulated in PTT. Importantly, the results of this study also showed significant changes in genes related to the crosstalk between autophagy, dormancy, and metastatic activity of treated cells. Our findings illustrated that PTT enhances the aggressiveness of cancer cells at 43 °C, in contrast to 48 °C by the regulation of autophagy-dependent manner. Nature Publishing Group UK 2021-12-14 /pmc/articles/PMC8671444/ /pubmed/34907215 http://dx.doi.org/10.1038/s41598-021-02697-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Ghafarkhani, Maryam
Avci, Cigir Biray
Rahbarghazi, Reza
Karimi, Abbas
Sadeghizadeh, Majid
Zarebkohan, Amir
Bani, Farhad
Mild hyperthermia induced by gold nanorods acts as a dual-edge blade in the fate of SH-SY5Y cells via autophagy
title Mild hyperthermia induced by gold nanorods acts as a dual-edge blade in the fate of SH-SY5Y cells via autophagy
title_full Mild hyperthermia induced by gold nanorods acts as a dual-edge blade in the fate of SH-SY5Y cells via autophagy
title_fullStr Mild hyperthermia induced by gold nanorods acts as a dual-edge blade in the fate of SH-SY5Y cells via autophagy
title_full_unstemmed Mild hyperthermia induced by gold nanorods acts as a dual-edge blade in the fate of SH-SY5Y cells via autophagy
title_short Mild hyperthermia induced by gold nanorods acts as a dual-edge blade in the fate of SH-SY5Y cells via autophagy
title_sort mild hyperthermia induced by gold nanorods acts as a dual-edge blade in the fate of sh-sy5y cells via autophagy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8671444/
https://www.ncbi.nlm.nih.gov/pubmed/34907215
http://dx.doi.org/10.1038/s41598-021-02697-y
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