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Long non-coding RNA MIAT is involved in the regulation of pyroptosis in diabetic cardiomyopathy via targeting miR-214-3p

Diabetic cardiomyopathy (DCM) is one of the most common complications of diabetes without effective treatment options. Its pathogenesis is complex and remains unclear. Long non-coding RNA (lncRNA) MIAT allele has been reported to be enriched in DCM patients and activate a pyroptosis program in hypox...

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Autores principales: Xiao, Wenjing, Zheng, Dezhi, Chen, Xin, Yu, Botao, Deng, Kaiwen, Ma, Jie, Wen, Xudong, Hu, Yonghe, Hou, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8671938/
https://www.ncbi.nlm.nih.gov/pubmed/34950859
http://dx.doi.org/10.1016/j.isci.2021.103518
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author Xiao, Wenjing
Zheng, Dezhi
Chen, Xin
Yu, Botao
Deng, Kaiwen
Ma, Jie
Wen, Xudong
Hu, Yonghe
Hou, Jun
author_facet Xiao, Wenjing
Zheng, Dezhi
Chen, Xin
Yu, Botao
Deng, Kaiwen
Ma, Jie
Wen, Xudong
Hu, Yonghe
Hou, Jun
author_sort Xiao, Wenjing
collection PubMed
description Diabetic cardiomyopathy (DCM) is one of the most common complications of diabetes without effective treatment options. Its pathogenesis is complex and remains unclear. Long non-coding RNA (lncRNA) MIAT allele has been reported to be enriched in DCM patients and activate a pyroptosis program in hypoxia-induced H9c2 cells. Thus, whether MIAT played a role in DCM pyroptosis remains to be clarified. In the study, the expression of MIAT was found elevated in the serum of diabetic patients, as well as in high-glucose induced cardiomyocytes and diabetic mice. Further, the expression levels of CASP1 and pyroptosis correlation factors (IL-1 and IL-18) were downregulated after silencing MIAT. Through modeling and validation experiments, we then confirmed that the MIAT-miR-214-3p-CASP1 axis serves as an essential point in pyroptosis of DCM mice. These results suggested that silencing MIAT would be a potential treatment strategy for DCM.
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spelling pubmed-86719382021-12-22 Long non-coding RNA MIAT is involved in the regulation of pyroptosis in diabetic cardiomyopathy via targeting miR-214-3p Xiao, Wenjing Zheng, Dezhi Chen, Xin Yu, Botao Deng, Kaiwen Ma, Jie Wen, Xudong Hu, Yonghe Hou, Jun iScience Article Diabetic cardiomyopathy (DCM) is one of the most common complications of diabetes without effective treatment options. Its pathogenesis is complex and remains unclear. Long non-coding RNA (lncRNA) MIAT allele has been reported to be enriched in DCM patients and activate a pyroptosis program in hypoxia-induced H9c2 cells. Thus, whether MIAT played a role in DCM pyroptosis remains to be clarified. In the study, the expression of MIAT was found elevated in the serum of diabetic patients, as well as in high-glucose induced cardiomyocytes and diabetic mice. Further, the expression levels of CASP1 and pyroptosis correlation factors (IL-1 and IL-18) were downregulated after silencing MIAT. Through modeling and validation experiments, we then confirmed that the MIAT-miR-214-3p-CASP1 axis serves as an essential point in pyroptosis of DCM mice. These results suggested that silencing MIAT would be a potential treatment strategy for DCM. Elsevier 2021-11-26 /pmc/articles/PMC8671938/ /pubmed/34950859 http://dx.doi.org/10.1016/j.isci.2021.103518 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Xiao, Wenjing
Zheng, Dezhi
Chen, Xin
Yu, Botao
Deng, Kaiwen
Ma, Jie
Wen, Xudong
Hu, Yonghe
Hou, Jun
Long non-coding RNA MIAT is involved in the regulation of pyroptosis in diabetic cardiomyopathy via targeting miR-214-3p
title Long non-coding RNA MIAT is involved in the regulation of pyroptosis in diabetic cardiomyopathy via targeting miR-214-3p
title_full Long non-coding RNA MIAT is involved in the regulation of pyroptosis in diabetic cardiomyopathy via targeting miR-214-3p
title_fullStr Long non-coding RNA MIAT is involved in the regulation of pyroptosis in diabetic cardiomyopathy via targeting miR-214-3p
title_full_unstemmed Long non-coding RNA MIAT is involved in the regulation of pyroptosis in diabetic cardiomyopathy via targeting miR-214-3p
title_short Long non-coding RNA MIAT is involved in the regulation of pyroptosis in diabetic cardiomyopathy via targeting miR-214-3p
title_sort long non-coding rna miat is involved in the regulation of pyroptosis in diabetic cardiomyopathy via targeting mir-214-3p
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8671938/
https://www.ncbi.nlm.nih.gov/pubmed/34950859
http://dx.doi.org/10.1016/j.isci.2021.103518
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