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Activation of a diluted E. coli cell-free transcription-translation system within liposomes by hypertonic concentration
We present a protocol for activating protein synthesis in liposomes encapsulating a diluted E. coli cell extract-based TX-TL (transcription-translation) system by hypertonic concentration. Protein expression is turned on in the liposome-encapsulated TX-TL system by simple treatment with a concentrat...
Autores principales: | Matsui, Yukino, Akui, Toshiki, Doi, Nobuhide, Fujiwara, Kei |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8672043/ https://www.ncbi.nlm.nih.gov/pubmed/34950885 http://dx.doi.org/10.1016/j.xpro.2021.101003 |
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