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Activation of a diluted E. coli cell-free transcription-translation system within liposomes by hypertonic concentration

We present a protocol for activating protein synthesis in liposomes encapsulating a diluted E. coli cell extract-based TX-TL (transcription-translation) system by hypertonic concentration. Protein expression is turned on in the liposome-encapsulated TX-TL system by simple treatment with a concentrat...

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Detalles Bibliográficos
Autores principales:	Matsui, Yukino, Akui, Toshiki, Doi, Nobuhide, Fujiwara, Kei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8672043/ https://www.ncbi.nlm.nih.gov/pubmed/34950885 http://dx.doi.org/10.1016/j.xpro.2021.101003

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