A detailed protocol for RNA cleavage assay in sympathetic neurons

This protocol illustrates the use of an in vitro assay to study the cleavage of the IMPA1 3′UTR by the endonuclease Ago2 in sympathetic neurons. The procedure includes the preparation of cytoplasmic protein extracts and also describes the synthesis and labeling of the RNA probe. The protocol can be...

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Detalles Bibliográficos
Autores principales: Andreassi, Catia, Riccio, Antonella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8672046/
https://www.ncbi.nlm.nih.gov/pubmed/34950884
http://dx.doi.org/10.1016/j.xpro.2021.101001
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author Andreassi, Catia
Riccio, Antonella
author_facet Andreassi, Catia
Riccio, Antonella
author_sort Andreassi, Catia
collection PubMed
description This protocol illustrates the use of an in vitro assay to study the cleavage of the IMPA1 3′UTR by the endonuclease Ago2 in sympathetic neurons. The procedure includes the preparation of cytoplasmic protein extracts and also describes the synthesis and labeling of the RNA probe. The protocol can be applied to other cell systems, RNA transcripts, and endonucleases to confirm the role of known cleavage site(s) and cleavage proteins, or to investigate new ones. For complete details on the use and execution of this protocol, please refer to Andreassi et al. (2021).
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spelling pubmed-86720462021-12-22 A detailed protocol for RNA cleavage assay in sympathetic neurons Andreassi, Catia Riccio, Antonella STAR Protoc Protocol This protocol illustrates the use of an in vitro assay to study the cleavage of the IMPA1 3′UTR by the endonuclease Ago2 in sympathetic neurons. The procedure includes the preparation of cytoplasmic protein extracts and also describes the synthesis and labeling of the RNA probe. The protocol can be applied to other cell systems, RNA transcripts, and endonucleases to confirm the role of known cleavage site(s) and cleavage proteins, or to investigate new ones. For complete details on the use and execution of this protocol, please refer to Andreassi et al. (2021). Elsevier 2021-12-11 /pmc/articles/PMC8672046/ /pubmed/34950884 http://dx.doi.org/10.1016/j.xpro.2021.101001 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Andreassi, Catia
Riccio, Antonella
A detailed protocol for RNA cleavage assay in sympathetic neurons
title A detailed protocol for RNA cleavage assay in sympathetic neurons
title_full A detailed protocol for RNA cleavage assay in sympathetic neurons
title_fullStr A detailed protocol for RNA cleavage assay in sympathetic neurons
title_full_unstemmed A detailed protocol for RNA cleavage assay in sympathetic neurons
title_short A detailed protocol for RNA cleavage assay in sympathetic neurons
title_sort detailed protocol for rna cleavage assay in sympathetic neurons
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8672046/
https://www.ncbi.nlm.nih.gov/pubmed/34950884
http://dx.doi.org/10.1016/j.xpro.2021.101001
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