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A protocol for imaging microvilli biogenesis on the surface of cultured porcine kidney epithelial cell monolayers
A key facet of epithelial differentiation is the assembly of actin-based protrusions known as microvilli, which amplify apical membrane surface area for various cell functions. To probe mechanisms of microvillus assembly, we developed a protocol using spinning disk confocal microscopy to directly vi...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8672049/ https://www.ncbi.nlm.nih.gov/pubmed/34950883 http://dx.doi.org/10.1016/j.xpro.2021.100998 |