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Protocol for cell type-specific labeling, enrichment, and proteomic profiling of plasma proteins in mice

Secreted polypeptides represent a fundamental axis of intercellular communication. Here, we present a protocol for the cell type-specific biotinylation, enrichment, and proteomic profiling of secreted plasma proteins directly in mice. This protocol uses conditional “turn-on” adeno-associated viruses...

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Detalles Bibliográficos
Autores principales: Wei, Wei, Riley, Nicholas M., Lyu, Xuchao, Bertozzi, Carolyn R., Long, Jonathan Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8672099/
https://www.ncbi.nlm.nih.gov/pubmed/34950890
http://dx.doi.org/10.1016/j.xpro.2021.101014