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HSP70 alleviates spinal cord injury by activating the NF-kB pathway

OBJECTIVES: Spinal cord injury (SCI) is an acute traumatic lesion of neurons in the spinal cord which has a high prevalence in the world, and has no effective surgical treatment. HSP70 is a molecular chaperone protein, serves a protective role in several different models of nervous system injury. Th...

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Autores principales: Xu, Bingqiang, Wang, Jun, Liu, Shizhang, Liu, Huitong, Zhang, Xiaoxia, Shi, Jiyuan, Ji, Le, Li, Jingyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Society of Musculoskeletal and Neuronal Interactions 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8672413/
https://www.ncbi.nlm.nih.gov/pubmed/34854394
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author Xu, Bingqiang
Wang, Jun
Liu, Shizhang
Liu, Huitong
Zhang, Xiaoxia
Shi, Jiyuan
Ji, Le
Li, Jingyuan
author_facet Xu, Bingqiang
Wang, Jun
Liu, Shizhang
Liu, Huitong
Zhang, Xiaoxia
Shi, Jiyuan
Ji, Le
Li, Jingyuan
author_sort Xu, Bingqiang
collection PubMed
description OBJECTIVES: Spinal cord injury (SCI) is an acute traumatic lesion of neurons in the spinal cord which has a high prevalence in the world, and has no effective surgical treatment. HSP70 is a molecular chaperone protein, serves a protective role in several different models of nervous system injury. The aim of the present study was to investigate the anti-inflammatory role of HSP70 in spinal cord injury and explore its mechanism. METHODS: In vivo and in vitro models were constructed to mimic SCI. The Basso Mouse Scale (BMS) was applied to assess SCI degrees of the mouse model. Immunofluorescence (IF) was used for visualizing HSP70 and Iba1 in the spinal cord. Western blot assay was employed to quantify HSP70 and p65, and ELISA was for IL-1β and TNF-α. RESULTS: The results showed that HSP70 expression decreased after SCI. HSP70 and Iba1 showed a decrease of co-localization in SCI mice. Further studies revealed that p65 was upregulated during the process of SCI. Overexpression of HSP70 inhibited the expression of p65 both in vitro and in vivo, and promoted the recovery of SCI mice. CONCLUSIONS: HSP70 was involved in the pathological process of spinal cord injury, HSP70 alleviated the spinal cord injury via inhibiting NF-κB signaling pathway.
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spelling pubmed-86724132021-12-20 HSP70 alleviates spinal cord injury by activating the NF-kB pathway Xu, Bingqiang Wang, Jun Liu, Shizhang Liu, Huitong Zhang, Xiaoxia Shi, Jiyuan Ji, Le Li, Jingyuan J Musculoskelet Neuronal Interact Original Article OBJECTIVES: Spinal cord injury (SCI) is an acute traumatic lesion of neurons in the spinal cord which has a high prevalence in the world, and has no effective surgical treatment. HSP70 is a molecular chaperone protein, serves a protective role in several different models of nervous system injury. The aim of the present study was to investigate the anti-inflammatory role of HSP70 in spinal cord injury and explore its mechanism. METHODS: In vivo and in vitro models were constructed to mimic SCI. The Basso Mouse Scale (BMS) was applied to assess SCI degrees of the mouse model. Immunofluorescence (IF) was used for visualizing HSP70 and Iba1 in the spinal cord. Western blot assay was employed to quantify HSP70 and p65, and ELISA was for IL-1β and TNF-α. RESULTS: The results showed that HSP70 expression decreased after SCI. HSP70 and Iba1 showed a decrease of co-localization in SCI mice. Further studies revealed that p65 was upregulated during the process of SCI. Overexpression of HSP70 inhibited the expression of p65 both in vitro and in vivo, and promoted the recovery of SCI mice. CONCLUSIONS: HSP70 was involved in the pathological process of spinal cord injury, HSP70 alleviated the spinal cord injury via inhibiting NF-κB signaling pathway. International Society of Musculoskeletal and Neuronal Interactions 2021 /pmc/articles/PMC8672413/ /pubmed/34854394 Text en Copyright: © Journal of Musculoskeletal and Neuronal Interactions https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 4.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Xu, Bingqiang
Wang, Jun
Liu, Shizhang
Liu, Huitong
Zhang, Xiaoxia
Shi, Jiyuan
Ji, Le
Li, Jingyuan
HSP70 alleviates spinal cord injury by activating the NF-kB pathway
title HSP70 alleviates spinal cord injury by activating the NF-kB pathway
title_full HSP70 alleviates spinal cord injury by activating the NF-kB pathway
title_fullStr HSP70 alleviates spinal cord injury by activating the NF-kB pathway
title_full_unstemmed HSP70 alleviates spinal cord injury by activating the NF-kB pathway
title_short HSP70 alleviates spinal cord injury by activating the NF-kB pathway
title_sort hsp70 alleviates spinal cord injury by activating the nf-kb pathway
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8672413/
https://www.ncbi.nlm.nih.gov/pubmed/34854394
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