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A naphthalimide-based peptide conjugate for concurrent imaging and apoptosis induction in cancer cells by utilizing endogenous hydrogen sulfide

The excessive production of endogenous hydrogen sulfide (H(2)S) in cancer cells leads to enhanced tumor growth and metastasis. On the other hand, decreased endogenous H(2)S suppresses tumor growth. The reported approaches for inhibiting tumor growth are selective silencing of the tumor-promoting gen...

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Detalles Bibliográficos
Autores principales: Singh, Narendra, Sharma, Swati, Singh, Ramesh, Rajput, Swati, Chattopadhyay, Naibedya, Tewari, Deepshikha, Joshi, Khashti Ballabh, Verma, Sandeep
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8672725/
https://www.ncbi.nlm.nih.gov/pubmed/35024130
http://dx.doi.org/10.1039/d1sc04030h
Descripción
Sumario:The excessive production of endogenous hydrogen sulfide (H(2)S) in cancer cells leads to enhanced tumor growth and metastasis. On the other hand, decreased endogenous H(2)S suppresses tumor growth. The reported approaches for inhibiting tumor growth are selective silencing of the tumor-promoting genes and pharmacological inhibition of these proteins. To enhance the antitumor efficacy of frontline chemotherapeutic agents, herein, we synthesized a highly sensitive endogenous H(2)S responsive fluorescent probe, i.e., a hydrogen sulfide-sensing naphthalimide-based peptide conjugate (HSNPc), which showed selective inhibition of proliferation of cancer cells due to apoptosis induction. Furthermore, HSNPc suppressed the glycolytic reserve, a critical energy source for the proliferation of cancer cells. HSNPc also decreased the Young's modulus of HeLa cells compared to the control cells, which demonstrated a direct relation between cell apoptosis and cell stiffness. Taken together, we demonstrated the dual function of detection and killing of cancer cells by HSNPc that can be likened to a theranostic role.