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Rapid establishment of a frontline field laboratory in response to an imported outbreak of Ebola virus disease in western Uganda, June 2019

The Democratic Republic of the Congo (DRC) declared an Ebola virus disease (EVD) outbreak in North Kivu in August 2018. By June 2019, the outbreak had spread to 26 health zones in northeastern DRC, causing >2,000 reported cases and >1,000 deaths. On June 10, 2019, three members of a Congolese...

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Autores principales: Schuh, Amy J., Kyondo, Jackson, Graziano, James, Balinandi, Stephen, Kainulainen, Markus H., Tumusiime, Alex, Nyakarahuka, Luke, Mulei, Sophia, Baluku, Jimmy, Lonergan, William, Mayer, Oren, Masereka, Rastus, Masereka, Fredrick, Businge, Esther, Gatare, Alphonse, Kabyanga, Loice, Muhindo, Samuel, Mugabe, Raymond, Makumbi, Issa, Kayiwa, Joshua, Wetaka, Milton Makoba, Brown, Vance, Ojwang, Joseph, Nelson, Lisa, Millard, Monica, Nichol, Stuart T., Montgomery, Joel M., Taboy, Celine H., Lutwama, Julius J., Klena, John D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8673597/
https://www.ncbi.nlm.nih.gov/pubmed/34860831
http://dx.doi.org/10.1371/journal.pntd.0009967
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author Schuh, Amy J.
Kyondo, Jackson
Graziano, James
Balinandi, Stephen
Kainulainen, Markus H.
Tumusiime, Alex
Nyakarahuka, Luke
Mulei, Sophia
Baluku, Jimmy
Lonergan, William
Mayer, Oren
Masereka, Rastus
Masereka, Fredrick
Businge, Esther
Gatare, Alphonse
Kabyanga, Loice
Muhindo, Samuel
Mugabe, Raymond
Makumbi, Issa
Kayiwa, Joshua
Wetaka, Milton Makoba
Brown, Vance
Ojwang, Joseph
Nelson, Lisa
Millard, Monica
Nichol, Stuart T.
Montgomery, Joel M.
Taboy, Celine H.
Lutwama, Julius J.
Klena, John D.
author_facet Schuh, Amy J.
Kyondo, Jackson
Graziano, James
Balinandi, Stephen
Kainulainen, Markus H.
Tumusiime, Alex
Nyakarahuka, Luke
Mulei, Sophia
Baluku, Jimmy
Lonergan, William
Mayer, Oren
Masereka, Rastus
Masereka, Fredrick
Businge, Esther
Gatare, Alphonse
Kabyanga, Loice
Muhindo, Samuel
Mugabe, Raymond
Makumbi, Issa
Kayiwa, Joshua
Wetaka, Milton Makoba
Brown, Vance
Ojwang, Joseph
Nelson, Lisa
Millard, Monica
Nichol, Stuart T.
Montgomery, Joel M.
Taboy, Celine H.
Lutwama, Julius J.
Klena, John D.
author_sort Schuh, Amy J.
collection PubMed
description The Democratic Republic of the Congo (DRC) declared an Ebola virus disease (EVD) outbreak in North Kivu in August 2018. By June 2019, the outbreak had spread to 26 health zones in northeastern DRC, causing >2,000 reported cases and >1,000 deaths. On June 10, 2019, three members of a Congolese family with EVD-like symptoms traveled to western Uganda’s Kasese District to seek medical care. Shortly thereafter, the Viral Hemorrhagic Fever Surveillance and Laboratory Program (VHF program) at the Uganda Virus Research Institute (UVRI) confirmed that all three patients had EVD. The Ugandan Ministry of Health declared an outbreak of EVD in Uganda’s Kasese District, notified the World Health Organization, and initiated a rapid response to contain the outbreak. As part of this response, UVRI and the United States Centers for Disease Control and Prevention, with the support of Uganda’s Public Health Emergency Operations Center, the Kasese District Health Team, the Superintendent of Bwera General Hospital, the United States Department of Defense’s Makerere University Walter Reed Project, and the United States Mission to Kampala’s Global Health Security Technical Working Group, jointly established an Ebola Field Laboratory in Kasese District at Bwera General Hospital, proximal to an Ebola Treatment Unit (ETU). The laboratory consisted of a rapid containment kit for viral inactivation of patient specimens and a GeneXpert Instrument for performing Xpert Ebola assays. Laboratory staff tested 76 specimens from alert and suspect cases of EVD; the majority were admitted to the ETU (89.3%) and reported recent travel to the DRC (58.9%). Although no EVD cases were detected by the field laboratory, it played an important role in patient management and epidemiological surveillance by providing diagnostic results in <3 hours. The integration of the field laboratory into Uganda’s National VHF Program also enabled patient specimens to be referred to Entebbe for confirmatory EBOV testing and testing for other hemorrhagic fever viruses that circulate in Uganda.
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spelling pubmed-86735972021-12-16 Rapid establishment of a frontline field laboratory in response to an imported outbreak of Ebola virus disease in western Uganda, June 2019 Schuh, Amy J. Kyondo, Jackson Graziano, James Balinandi, Stephen Kainulainen, Markus H. Tumusiime, Alex Nyakarahuka, Luke Mulei, Sophia Baluku, Jimmy Lonergan, William Mayer, Oren Masereka, Rastus Masereka, Fredrick Businge, Esther Gatare, Alphonse Kabyanga, Loice Muhindo, Samuel Mugabe, Raymond Makumbi, Issa Kayiwa, Joshua Wetaka, Milton Makoba Brown, Vance Ojwang, Joseph Nelson, Lisa Millard, Monica Nichol, Stuart T. Montgomery, Joel M. Taboy, Celine H. Lutwama, Julius J. Klena, John D. PLoS Negl Trop Dis Research Article The Democratic Republic of the Congo (DRC) declared an Ebola virus disease (EVD) outbreak in North Kivu in August 2018. By June 2019, the outbreak had spread to 26 health zones in northeastern DRC, causing >2,000 reported cases and >1,000 deaths. On June 10, 2019, three members of a Congolese family with EVD-like symptoms traveled to western Uganda’s Kasese District to seek medical care. Shortly thereafter, the Viral Hemorrhagic Fever Surveillance and Laboratory Program (VHF program) at the Uganda Virus Research Institute (UVRI) confirmed that all three patients had EVD. The Ugandan Ministry of Health declared an outbreak of EVD in Uganda’s Kasese District, notified the World Health Organization, and initiated a rapid response to contain the outbreak. As part of this response, UVRI and the United States Centers for Disease Control and Prevention, with the support of Uganda’s Public Health Emergency Operations Center, the Kasese District Health Team, the Superintendent of Bwera General Hospital, the United States Department of Defense’s Makerere University Walter Reed Project, and the United States Mission to Kampala’s Global Health Security Technical Working Group, jointly established an Ebola Field Laboratory in Kasese District at Bwera General Hospital, proximal to an Ebola Treatment Unit (ETU). The laboratory consisted of a rapid containment kit for viral inactivation of patient specimens and a GeneXpert Instrument for performing Xpert Ebola assays. Laboratory staff tested 76 specimens from alert and suspect cases of EVD; the majority were admitted to the ETU (89.3%) and reported recent travel to the DRC (58.9%). Although no EVD cases were detected by the field laboratory, it played an important role in patient management and epidemiological surveillance by providing diagnostic results in <3 hours. The integration of the field laboratory into Uganda’s National VHF Program also enabled patient specimens to be referred to Entebbe for confirmatory EBOV testing and testing for other hemorrhagic fever viruses that circulate in Uganda. Public Library of Science 2021-12-03 /pmc/articles/PMC8673597/ /pubmed/34860831 http://dx.doi.org/10.1371/journal.pntd.0009967 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Schuh, Amy J.
Kyondo, Jackson
Graziano, James
Balinandi, Stephen
Kainulainen, Markus H.
Tumusiime, Alex
Nyakarahuka, Luke
Mulei, Sophia
Baluku, Jimmy
Lonergan, William
Mayer, Oren
Masereka, Rastus
Masereka, Fredrick
Businge, Esther
Gatare, Alphonse
Kabyanga, Loice
Muhindo, Samuel
Mugabe, Raymond
Makumbi, Issa
Kayiwa, Joshua
Wetaka, Milton Makoba
Brown, Vance
Ojwang, Joseph
Nelson, Lisa
Millard, Monica
Nichol, Stuart T.
Montgomery, Joel M.
Taboy, Celine H.
Lutwama, Julius J.
Klena, John D.
Rapid establishment of a frontline field laboratory in response to an imported outbreak of Ebola virus disease in western Uganda, June 2019
title Rapid establishment of a frontline field laboratory in response to an imported outbreak of Ebola virus disease in western Uganda, June 2019
title_full Rapid establishment of a frontline field laboratory in response to an imported outbreak of Ebola virus disease in western Uganda, June 2019
title_fullStr Rapid establishment of a frontline field laboratory in response to an imported outbreak of Ebola virus disease in western Uganda, June 2019
title_full_unstemmed Rapid establishment of a frontline field laboratory in response to an imported outbreak of Ebola virus disease in western Uganda, June 2019
title_short Rapid establishment of a frontline field laboratory in response to an imported outbreak of Ebola virus disease in western Uganda, June 2019
title_sort rapid establishment of a frontline field laboratory in response to an imported outbreak of ebola virus disease in western uganda, june 2019
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8673597/
https://www.ncbi.nlm.nih.gov/pubmed/34860831
http://dx.doi.org/10.1371/journal.pntd.0009967
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