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Derivation of pb(II)-sensing Escherichia coli cell-based biosensors from arsenic responsive genetic systems

Heavy metal-responsive operons were used for the generation of Escherichia coli cell-based biosensors. The selectivity and specificity of the biosensors were determined based on the interaction between heavy metals and regulatory proteins; thereby, the modulating target selectivity of biosensors cou...

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Autores principales: Lee, Yejin, Jeon, Yangwon, Jang, Guepil, Yoon, Youngdae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8674403/
https://www.ncbi.nlm.nih.gov/pubmed/34910261
http://dx.doi.org/10.1186/s13568-021-01329-y
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author Lee, Yejin
Jeon, Yangwon
Jang, Guepil
Yoon, Youngdae
author_facet Lee, Yejin
Jeon, Yangwon
Jang, Guepil
Yoon, Youngdae
author_sort Lee, Yejin
collection PubMed
description Heavy metal-responsive operons were used for the generation of Escherichia coli cell-based biosensors. The selectivity and specificity of the biosensors were determined based on the interaction between heavy metals and regulatory proteins; thereby, the modulating target selectivity of biosensors could be achieved by changing target sensing properties of regulatory proteins. The results of this study demonstrated that Pb(II)-sensing biosensors could be generated from an arsenic-responsive genetic system, which was originally used for arsenic-sensing biosensors. The amino acids around to As(III)-binding sites of ArsR were mutated and cysteine residues were relocated to modulate the metal selectivity. In addition, genes encoding metal ion-translocating P-type ATPases, such as copA and zntA, were deleted to enhance the specificity by increasing the intercellular levels of divalent metal ions. Based on the results, channel protein deleted E. coli cells harboring a pair of recombinant genes, engineered ArsR and arsAp::egfp, showed enhanced responses upon Pb exposure and could be used to quantify the amount of Pb(II) in artificially contaminated water and plants grown in media containing Pb(II). Although we focused on generating Pb(II)-specific biosensors in this study, the proposed strategy has a great potential for the generation of diverse heavy metal-sensing biosensors and risk assessment of heavy metals in environmental samples as well as in plants. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13568-021-01329-y.
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spelling pubmed-86744032021-12-17 Derivation of pb(II)-sensing Escherichia coli cell-based biosensors from arsenic responsive genetic systems Lee, Yejin Jeon, Yangwon Jang, Guepil Yoon, Youngdae AMB Express Original Article Heavy metal-responsive operons were used for the generation of Escherichia coli cell-based biosensors. The selectivity and specificity of the biosensors were determined based on the interaction between heavy metals and regulatory proteins; thereby, the modulating target selectivity of biosensors could be achieved by changing target sensing properties of regulatory proteins. The results of this study demonstrated that Pb(II)-sensing biosensors could be generated from an arsenic-responsive genetic system, which was originally used for arsenic-sensing biosensors. The amino acids around to As(III)-binding sites of ArsR were mutated and cysteine residues were relocated to modulate the metal selectivity. In addition, genes encoding metal ion-translocating P-type ATPases, such as copA and zntA, were deleted to enhance the specificity by increasing the intercellular levels of divalent metal ions. Based on the results, channel protein deleted E. coli cells harboring a pair of recombinant genes, engineered ArsR and arsAp::egfp, showed enhanced responses upon Pb exposure and could be used to quantify the amount of Pb(II) in artificially contaminated water and plants grown in media containing Pb(II). Although we focused on generating Pb(II)-specific biosensors in this study, the proposed strategy has a great potential for the generation of diverse heavy metal-sensing biosensors and risk assessment of heavy metals in environmental samples as well as in plants. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13568-021-01329-y. Springer Berlin Heidelberg 2021-12-15 /pmc/articles/PMC8674403/ /pubmed/34910261 http://dx.doi.org/10.1186/s13568-021-01329-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Lee, Yejin
Jeon, Yangwon
Jang, Guepil
Yoon, Youngdae
Derivation of pb(II)-sensing Escherichia coli cell-based biosensors from arsenic responsive genetic systems
title Derivation of pb(II)-sensing Escherichia coli cell-based biosensors from arsenic responsive genetic systems
title_full Derivation of pb(II)-sensing Escherichia coli cell-based biosensors from arsenic responsive genetic systems
title_fullStr Derivation of pb(II)-sensing Escherichia coli cell-based biosensors from arsenic responsive genetic systems
title_full_unstemmed Derivation of pb(II)-sensing Escherichia coli cell-based biosensors from arsenic responsive genetic systems
title_short Derivation of pb(II)-sensing Escherichia coli cell-based biosensors from arsenic responsive genetic systems
title_sort derivation of pb(ii)-sensing escherichia coli cell-based biosensors from arsenic responsive genetic systems
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8674403/
https://www.ncbi.nlm.nih.gov/pubmed/34910261
http://dx.doi.org/10.1186/s13568-021-01329-y
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