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A Novel Archaeal Lineage in Boiling Hot Springs around Oyasukyo Gorge (Akita, Japan)

A novel deep-branching archaeal lineage was discovered at high-temperature hot springs around Oyasukyo Gorge in Akita Prefecture, Japan. Actively boiling hot spring water contained >1×10(4) microbes mL(–1). The microbial community composition assessed by analyzing 16S rRNA gene amplicons revealed...

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Detalles Bibliográficos
Autores principales: Asamatsu, Katsuhiro, Yoshitake, Kai, Saito, Makoto, Prasitwuttisak, Wipoo, Ishibashi, Jun-ichiro, Tsutsumi, Akihi, Mustapha, Nurul Asyifah, Maeda, Toshinari, Yanagawa, Katsunori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8674440/
https://www.ncbi.nlm.nih.gov/pubmed/34819404
http://dx.doi.org/10.1264/jsme2.ME21048
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author Asamatsu, Katsuhiro
Yoshitake, Kai
Saito, Makoto
Prasitwuttisak, Wipoo
Ishibashi, Jun-ichiro
Tsutsumi, Akihi
Mustapha, Nurul Asyifah
Maeda, Toshinari
Yanagawa, Katsunori
author_facet Asamatsu, Katsuhiro
Yoshitake, Kai
Saito, Makoto
Prasitwuttisak, Wipoo
Ishibashi, Jun-ichiro
Tsutsumi, Akihi
Mustapha, Nurul Asyifah
Maeda, Toshinari
Yanagawa, Katsunori
author_sort Asamatsu, Katsuhiro
collection PubMed
description A novel deep-branching archaeal lineage was discovered at high-temperature hot springs around Oyasukyo Gorge in Akita Prefecture, Japan. Actively boiling hot spring water contained >1×10(4) microbes mL(–1). The microbial community composition assessed by analyzing 16S rRNA gene amplicons revealed that the dominant bacterial phyla were Proteobacteria and Aquificae (>50% of the microbial composition) in samples collected in 2016 and 2019, respectively. Approximately 10% of the reads obtained in both years were not assigned to any taxonomy. The more detailed phylogenetic positions of the unassigned sequences identified using a clone library and phylogenetic tree showed that they formed a clade that was independent, distantly related to known phyla, and had low similarity (<82%) to all other sequences in available databases. The present results suggest that this novel archaeal phylum-level lineage thrives in boiling hot springs in Japan.
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spelling pubmed-86744402021-12-23 A Novel Archaeal Lineage in Boiling Hot Springs around Oyasukyo Gorge (Akita, Japan) Asamatsu, Katsuhiro Yoshitake, Kai Saito, Makoto Prasitwuttisak, Wipoo Ishibashi, Jun-ichiro Tsutsumi, Akihi Mustapha, Nurul Asyifah Maeda, Toshinari Yanagawa, Katsunori Microbes Environ Regular Paper A novel deep-branching archaeal lineage was discovered at high-temperature hot springs around Oyasukyo Gorge in Akita Prefecture, Japan. Actively boiling hot spring water contained >1×10(4) microbes mL(–1). The microbial community composition assessed by analyzing 16S rRNA gene amplicons revealed that the dominant bacterial phyla were Proteobacteria and Aquificae (>50% of the microbial composition) in samples collected in 2016 and 2019, respectively. Approximately 10% of the reads obtained in both years were not assigned to any taxonomy. The more detailed phylogenetic positions of the unassigned sequences identified using a clone library and phylogenetic tree showed that they formed a clade that was independent, distantly related to known phyla, and had low similarity (<82%) to all other sequences in available databases. The present results suggest that this novel archaeal phylum-level lineage thrives in boiling hot springs in Japan. Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2021 2021-11-25 /pmc/articles/PMC8674440/ /pubmed/34819404 http://dx.doi.org/10.1264/jsme2.ME21048 Text en 2021 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Regular Paper
Asamatsu, Katsuhiro
Yoshitake, Kai
Saito, Makoto
Prasitwuttisak, Wipoo
Ishibashi, Jun-ichiro
Tsutsumi, Akihi
Mustapha, Nurul Asyifah
Maeda, Toshinari
Yanagawa, Katsunori
A Novel Archaeal Lineage in Boiling Hot Springs around Oyasukyo Gorge (Akita, Japan)
title A Novel Archaeal Lineage in Boiling Hot Springs around Oyasukyo Gorge (Akita, Japan)
title_full A Novel Archaeal Lineage in Boiling Hot Springs around Oyasukyo Gorge (Akita, Japan)
title_fullStr A Novel Archaeal Lineage in Boiling Hot Springs around Oyasukyo Gorge (Akita, Japan)
title_full_unstemmed A Novel Archaeal Lineage in Boiling Hot Springs around Oyasukyo Gorge (Akita, Japan)
title_short A Novel Archaeal Lineage in Boiling Hot Springs around Oyasukyo Gorge (Akita, Japan)
title_sort novel archaeal lineage in boiling hot springs around oyasukyo gorge (akita, japan)
topic Regular Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8674440/
https://www.ncbi.nlm.nih.gov/pubmed/34819404
http://dx.doi.org/10.1264/jsme2.ME21048
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