Cargando…

The Antagonism of Corticotropin-Releasing Factor Receptor-1 in Brain Suppress Stress-Induced Propofol Self-Administration in Rats

Propofol addiction has been detected in humans and rats, which may be facilitated by stress. Corticotropin-releasing factor acts through the corticotropin-releasing factor (CRF) receptor-1 (CRF1R) and CRF2 receptor-2 (CRF2R) and is a crucial candidate target for the interaction between stress and dr...

Descripción completa

Detalles Bibliográficos
Autores principales: Dong, Zhanglei, Zhang, Gaolong, Xiang, Saiqiong, Jiang, Chenchen, Chen, Zhichuan, Li, Yan, Huang, Bingwu, Zhou, Wenhua, Lian, Qingquan, Wu, Binbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8674615/
https://www.ncbi.nlm.nih.gov/pubmed/34924971
http://dx.doi.org/10.3389/fnbeh.2021.775209
_version_ 1784615710436098048
author Dong, Zhanglei
Zhang, Gaolong
Xiang, Saiqiong
Jiang, Chenchen
Chen, Zhichuan
Li, Yan
Huang, Bingwu
Zhou, Wenhua
Lian, Qingquan
Wu, Binbin
author_facet Dong, Zhanglei
Zhang, Gaolong
Xiang, Saiqiong
Jiang, Chenchen
Chen, Zhichuan
Li, Yan
Huang, Bingwu
Zhou, Wenhua
Lian, Qingquan
Wu, Binbin
author_sort Dong, Zhanglei
collection PubMed
description Propofol addiction has been detected in humans and rats, which may be facilitated by stress. Corticotropin-releasing factor acts through the corticotropin-releasing factor (CRF) receptor-1 (CRF1R) and CRF2 receptor-2 (CRF2R) and is a crucial candidate target for the interaction between stress and drug abuse, but its role on propofol addiction remains unknown. Tail clip stressful stimulation was performed in rats to test the stress on the establishment of the propofol self-administration behavioral model. Thereafter, the rats were pretreated before the testing session at the bilateral lateral ventricle with one of the doses of antalarmin (CRF1R antagonist, 100–500 ng/site), antisauvagine 30 (CRF2R antagonist, 100–500 ng/site), and RU486 (glucocorticoid receptor antagonist, 100–500 ng/site) or vehicle. The dopamine D1 receptor (D1R) in the nucleus accumbens (NAc) was detected to explore the underlying molecular mechanism. The sucrose self-administration establishment and maintenance, and locomotor activities were also examined to determine the specificity. We found that the establishment of propofol self-administration was promoted in the tail clip treated group (the stress group), which was inhibited by antalarmin at the dose of 100–500 ng/site but was not by antisauvagine 30 or RU486. Accordingly, the expression of D1R in the NAc was attenuated by antalarmin, dose-dependently. Moreover, pretreatments fail to change sucrose self-administration behavior or locomotor activities. This study supports the role of CRF1R in the brain in mediating the central reward processing through D1R in the NAc and provided a possibility that CRF1R antagonist may be a new therapeutic approach for the treatment of propofol addiction.
format Online
Article
Text
id pubmed-8674615
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-86746152021-12-17 The Antagonism of Corticotropin-Releasing Factor Receptor-1 in Brain Suppress Stress-Induced Propofol Self-Administration in Rats Dong, Zhanglei Zhang, Gaolong Xiang, Saiqiong Jiang, Chenchen Chen, Zhichuan Li, Yan Huang, Bingwu Zhou, Wenhua Lian, Qingquan Wu, Binbin Front Behav Neurosci Neuroscience Propofol addiction has been detected in humans and rats, which may be facilitated by stress. Corticotropin-releasing factor acts through the corticotropin-releasing factor (CRF) receptor-1 (CRF1R) and CRF2 receptor-2 (CRF2R) and is a crucial candidate target for the interaction between stress and drug abuse, but its role on propofol addiction remains unknown. Tail clip stressful stimulation was performed in rats to test the stress on the establishment of the propofol self-administration behavioral model. Thereafter, the rats were pretreated before the testing session at the bilateral lateral ventricle with one of the doses of antalarmin (CRF1R antagonist, 100–500 ng/site), antisauvagine 30 (CRF2R antagonist, 100–500 ng/site), and RU486 (glucocorticoid receptor antagonist, 100–500 ng/site) or vehicle. The dopamine D1 receptor (D1R) in the nucleus accumbens (NAc) was detected to explore the underlying molecular mechanism. The sucrose self-administration establishment and maintenance, and locomotor activities were also examined to determine the specificity. We found that the establishment of propofol self-administration was promoted in the tail clip treated group (the stress group), which was inhibited by antalarmin at the dose of 100–500 ng/site but was not by antisauvagine 30 or RU486. Accordingly, the expression of D1R in the NAc was attenuated by antalarmin, dose-dependently. Moreover, pretreatments fail to change sucrose self-administration behavior or locomotor activities. This study supports the role of CRF1R in the brain in mediating the central reward processing through D1R in the NAc and provided a possibility that CRF1R antagonist may be a new therapeutic approach for the treatment of propofol addiction. Frontiers Media S.A. 2021-12-02 /pmc/articles/PMC8674615/ /pubmed/34924971 http://dx.doi.org/10.3389/fnbeh.2021.775209 Text en Copyright © 2021 Dong, Zhang, Xiang, Jiang, Chen, Li, Huang, Zhou, Lian and Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Dong, Zhanglei
Zhang, Gaolong
Xiang, Saiqiong
Jiang, Chenchen
Chen, Zhichuan
Li, Yan
Huang, Bingwu
Zhou, Wenhua
Lian, Qingquan
Wu, Binbin
The Antagonism of Corticotropin-Releasing Factor Receptor-1 in Brain Suppress Stress-Induced Propofol Self-Administration in Rats
title The Antagonism of Corticotropin-Releasing Factor Receptor-1 in Brain Suppress Stress-Induced Propofol Self-Administration in Rats
title_full The Antagonism of Corticotropin-Releasing Factor Receptor-1 in Brain Suppress Stress-Induced Propofol Self-Administration in Rats
title_fullStr The Antagonism of Corticotropin-Releasing Factor Receptor-1 in Brain Suppress Stress-Induced Propofol Self-Administration in Rats
title_full_unstemmed The Antagonism of Corticotropin-Releasing Factor Receptor-1 in Brain Suppress Stress-Induced Propofol Self-Administration in Rats
title_short The Antagonism of Corticotropin-Releasing Factor Receptor-1 in Brain Suppress Stress-Induced Propofol Self-Administration in Rats
title_sort antagonism of corticotropin-releasing factor receptor-1 in brain suppress stress-induced propofol self-administration in rats
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8674615/
https://www.ncbi.nlm.nih.gov/pubmed/34924971
http://dx.doi.org/10.3389/fnbeh.2021.775209
work_keys_str_mv AT dongzhanglei theantagonismofcorticotropinreleasingfactorreceptor1inbrainsuppressstressinducedpropofolselfadministrationinrats
AT zhanggaolong theantagonismofcorticotropinreleasingfactorreceptor1inbrainsuppressstressinducedpropofolselfadministrationinrats
AT xiangsaiqiong theantagonismofcorticotropinreleasingfactorreceptor1inbrainsuppressstressinducedpropofolselfadministrationinrats
AT jiangchenchen theantagonismofcorticotropinreleasingfactorreceptor1inbrainsuppressstressinducedpropofolselfadministrationinrats
AT chenzhichuan theantagonismofcorticotropinreleasingfactorreceptor1inbrainsuppressstressinducedpropofolselfadministrationinrats
AT liyan theantagonismofcorticotropinreleasingfactorreceptor1inbrainsuppressstressinducedpropofolselfadministrationinrats
AT huangbingwu theantagonismofcorticotropinreleasingfactorreceptor1inbrainsuppressstressinducedpropofolselfadministrationinrats
AT zhouwenhua theantagonismofcorticotropinreleasingfactorreceptor1inbrainsuppressstressinducedpropofolselfadministrationinrats
AT lianqingquan theantagonismofcorticotropinreleasingfactorreceptor1inbrainsuppressstressinducedpropofolselfadministrationinrats
AT wubinbin theantagonismofcorticotropinreleasingfactorreceptor1inbrainsuppressstressinducedpropofolselfadministrationinrats
AT dongzhanglei antagonismofcorticotropinreleasingfactorreceptor1inbrainsuppressstressinducedpropofolselfadministrationinrats
AT zhanggaolong antagonismofcorticotropinreleasingfactorreceptor1inbrainsuppressstressinducedpropofolselfadministrationinrats
AT xiangsaiqiong antagonismofcorticotropinreleasingfactorreceptor1inbrainsuppressstressinducedpropofolselfadministrationinrats
AT jiangchenchen antagonismofcorticotropinreleasingfactorreceptor1inbrainsuppressstressinducedpropofolselfadministrationinrats
AT chenzhichuan antagonismofcorticotropinreleasingfactorreceptor1inbrainsuppressstressinducedpropofolselfadministrationinrats
AT liyan antagonismofcorticotropinreleasingfactorreceptor1inbrainsuppressstressinducedpropofolselfadministrationinrats
AT huangbingwu antagonismofcorticotropinreleasingfactorreceptor1inbrainsuppressstressinducedpropofolselfadministrationinrats
AT zhouwenhua antagonismofcorticotropinreleasingfactorreceptor1inbrainsuppressstressinducedpropofolselfadministrationinrats
AT lianqingquan antagonismofcorticotropinreleasingfactorreceptor1inbrainsuppressstressinducedpropofolselfadministrationinrats
AT wubinbin antagonismofcorticotropinreleasingfactorreceptor1inbrainsuppressstressinducedpropofolselfadministrationinrats