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Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles

Amphibians are sensitive to deteriorating environmental conditions, especially during transition to a terrestrial environment which is full of uncertainties. Harsh conditions, such as desiccation during earlier stages, affect different larval traits with possible carry-over effects on juvenile and a...

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Autores principales: Prokić, Marko D., Petrović, Tamara G., Gavrilović, Branka R., Despotović, Svetlana G., Gavrić, Jelena P., Kijanović, Ana, Tomašević Kolarov, Nataša, Vukov, Tanja, Radovanović, Tijana B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8674722/
https://www.ncbi.nlm.nih.gov/pubmed/34925072
http://dx.doi.org/10.3389/fphys.2021.783288
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author Prokić, Marko D.
Petrović, Tamara G.
Gavrilović, Branka R.
Despotović, Svetlana G.
Gavrić, Jelena P.
Kijanović, Ana
Tomašević Kolarov, Nataša
Vukov, Tanja
Radovanović, Tijana B.
author_facet Prokić, Marko D.
Petrović, Tamara G.
Gavrilović, Branka R.
Despotović, Svetlana G.
Gavrić, Jelena P.
Kijanović, Ana
Tomašević Kolarov, Nataša
Vukov, Tanja
Radovanović, Tijana B.
author_sort Prokić, Marko D.
collection PubMed
description Amphibians are sensitive to deteriorating environmental conditions, especially during transition to a terrestrial environment which is full of uncertainties. Harsh conditions, such as desiccation during earlier stages, affect different larval traits with possible carry-over effects on juvenile and adult life histories. The first consequences of the effects can be seen in juveniles in the challenges to find food and the ability to survive without it in a terrestrial habitat. Body size and the internal energy reserves acquired during the larval phase play an important role in this period. Herein, we tested how different water regimes (low water availability, desiccation and constant high-water availability) during larval development reflect on the oxidative status and ability of yellow belly toad (Bombina variegata) juveniles to endure short-term fasting. The desiccation regime significantly reduced the body size of metamorphs. The same was observed after 2 weeks of fasting, while the feeding treatment reduced differences mostly in the body mass of individuals from different water regimes. This was the result of a greater gain in mass in juveniles pre-exposed to desiccation. Pre-exposure to desiccation also modified the parameters of the antioxidant system (AOS) under feeding conditions, leading to higher values of superoxide dismutase, glutathione reductase and glutathione S-transferase, glutathione and sulfhydryl group concentrations, and lower glutathione peroxidase in comparison to juveniles reared under constant water. The increase in the AOS of juveniles can be considered as a physiological carry-over effect of desiccation, probably as the result of compensatory growth and/or earlier exposure to chronic stress. However, water levels during larval development did not exert significant effects on the oxidative status of juveniles subjected to food unavailability. Fasting juveniles, both control and desiccated, were exposed to oxidative stress, significantly higher lipid peroxide concentrations, lower superoxide dismutase, glutathione peroxidase, glutathione S-transferase, glutathione and sulfhydryl group values in comparison to feeding individuals. The lack of food in juvenile anurans activated the AOS response in the same manner, regardless of body size and stress pre-exposure, suggesting that the generally accepted hypothesis about the influence of metamorphic body size on the fitness of the postmetamorphic stage should be tested further.
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spelling pubmed-86747222021-12-17 Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles Prokić, Marko D. Petrović, Tamara G. Gavrilović, Branka R. Despotović, Svetlana G. Gavrić, Jelena P. Kijanović, Ana Tomašević Kolarov, Nataša Vukov, Tanja Radovanović, Tijana B. Front Physiol Physiology Amphibians are sensitive to deteriorating environmental conditions, especially during transition to a terrestrial environment which is full of uncertainties. Harsh conditions, such as desiccation during earlier stages, affect different larval traits with possible carry-over effects on juvenile and adult life histories. The first consequences of the effects can be seen in juveniles in the challenges to find food and the ability to survive without it in a terrestrial habitat. Body size and the internal energy reserves acquired during the larval phase play an important role in this period. Herein, we tested how different water regimes (low water availability, desiccation and constant high-water availability) during larval development reflect on the oxidative status and ability of yellow belly toad (Bombina variegata) juveniles to endure short-term fasting. The desiccation regime significantly reduced the body size of metamorphs. The same was observed after 2 weeks of fasting, while the feeding treatment reduced differences mostly in the body mass of individuals from different water regimes. This was the result of a greater gain in mass in juveniles pre-exposed to desiccation. Pre-exposure to desiccation also modified the parameters of the antioxidant system (AOS) under feeding conditions, leading to higher values of superoxide dismutase, glutathione reductase and glutathione S-transferase, glutathione and sulfhydryl group concentrations, and lower glutathione peroxidase in comparison to juveniles reared under constant water. The increase in the AOS of juveniles can be considered as a physiological carry-over effect of desiccation, probably as the result of compensatory growth and/or earlier exposure to chronic stress. However, water levels during larval development did not exert significant effects on the oxidative status of juveniles subjected to food unavailability. Fasting juveniles, both control and desiccated, were exposed to oxidative stress, significantly higher lipid peroxide concentrations, lower superoxide dismutase, glutathione peroxidase, glutathione S-transferase, glutathione and sulfhydryl group values in comparison to feeding individuals. The lack of food in juvenile anurans activated the AOS response in the same manner, regardless of body size and stress pre-exposure, suggesting that the generally accepted hypothesis about the influence of metamorphic body size on the fitness of the postmetamorphic stage should be tested further. Frontiers Media S.A. 2021-12-02 /pmc/articles/PMC8674722/ /pubmed/34925072 http://dx.doi.org/10.3389/fphys.2021.783288 Text en Copyright © 2021 Prokić, Petrović, Gavrilović, Despotović, Gavrić, Kijanović, Tomašević Kolarov, Vukov and Radovanović. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Prokić, Marko D.
Petrović, Tamara G.
Gavrilović, Branka R.
Despotović, Svetlana G.
Gavrić, Jelena P.
Kijanović, Ana
Tomašević Kolarov, Nataša
Vukov, Tanja
Radovanović, Tijana B.
Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles
title Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles
title_full Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles
title_fullStr Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles
title_full_unstemmed Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles
title_short Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles
title_sort carry-over effects of desiccation stress on the oxidative status of fasting anuran juveniles
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8674722/
https://www.ncbi.nlm.nih.gov/pubmed/34925072
http://dx.doi.org/10.3389/fphys.2021.783288
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