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Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis

Hashimoto’s thyroiditis (HT) is an autoimmune disease, and its incidence continues to rise. Although scientists have studied this disease for many years and discovered the potential effects of various proteins in it, the specific pathogenesis is still not fully comprehended. To understand HT and tra...

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Autores principales: Xiao, Hui, Liang, Jianqing, Liu, Sunqiang, Zhang, Qiongyue, Xie, Famin, Kong, Xingyu, Guo, Shanshan, Wang, Ruwen, Fu, Rong, Ye, Zhiqi, Li, Yun, Zhang, Shuang, Zhang, Li, Kaudimba, Keneilwe Kenny, Wang, Ru, Kong, Xingxing, Zhao, Bing, Zheng, Xuqin, Liu, Tiemin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8674930/
https://www.ncbi.nlm.nih.gov/pubmed/34925365
http://dx.doi.org/10.3389/fimmu.2021.784975
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author Xiao, Hui
Liang, Jianqing
Liu, Sunqiang
Zhang, Qiongyue
Xie, Famin
Kong, Xingyu
Guo, Shanshan
Wang, Ruwen
Fu, Rong
Ye, Zhiqi
Li, Yun
Zhang, Shuang
Zhang, Li
Kaudimba, Keneilwe Kenny
Wang, Ru
Kong, Xingxing
Zhao, Bing
Zheng, Xuqin
Liu, Tiemin
author_facet Xiao, Hui
Liang, Jianqing
Liu, Sunqiang
Zhang, Qiongyue
Xie, Famin
Kong, Xingyu
Guo, Shanshan
Wang, Ruwen
Fu, Rong
Ye, Zhiqi
Li, Yun
Zhang, Shuang
Zhang, Li
Kaudimba, Keneilwe Kenny
Wang, Ru
Kong, Xingxing
Zhao, Bing
Zheng, Xuqin
Liu, Tiemin
author_sort Xiao, Hui
collection PubMed
description Hashimoto’s thyroiditis (HT) is an autoimmune disease, and its incidence continues to rise. Although scientists have studied this disease for many years and discovered the potential effects of various proteins in it, the specific pathogenesis is still not fully comprehended. To understand HT and translate this knowledge to clinical applications, we took the mass spectrometric analysis on thyroid tissue fine-needle puncture from HT patients and healthy people in an attempt to make a further understanding of the pathogenesis of HT. A total of 44 proteins with differential expression were identified in HT patients, and these proteins play vital roles in cell adhesion, cell metabolism, and thyroxine synthesis. Combining patient clinical trial sample information, we further compared the transient changes of gene expression regulation in HT and papillary thyroid carcinoma (PTC) samples. More importantly, we developed patient-derived HT and PTC organoids as a promising new preclinical model to verify these potential markers. Our data revealed a marked characteristic of HT organoid in upregulating chemokines that include C-C motif chemokine ligand (CCL) 2 and CCL3, which play a key role in the pathogenesis of HT. Overall, our research has enriched everyone’s understanding of the pathogenesis of HT and provides a certain reference for the treatment of the disease.
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spelling pubmed-86749302021-12-17 Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis Xiao, Hui Liang, Jianqing Liu, Sunqiang Zhang, Qiongyue Xie, Famin Kong, Xingyu Guo, Shanshan Wang, Ruwen Fu, Rong Ye, Zhiqi Li, Yun Zhang, Shuang Zhang, Li Kaudimba, Keneilwe Kenny Wang, Ru Kong, Xingxing Zhao, Bing Zheng, Xuqin Liu, Tiemin Front Immunol Immunology Hashimoto’s thyroiditis (HT) is an autoimmune disease, and its incidence continues to rise. Although scientists have studied this disease for many years and discovered the potential effects of various proteins in it, the specific pathogenesis is still not fully comprehended. To understand HT and translate this knowledge to clinical applications, we took the mass spectrometric analysis on thyroid tissue fine-needle puncture from HT patients and healthy people in an attempt to make a further understanding of the pathogenesis of HT. A total of 44 proteins with differential expression were identified in HT patients, and these proteins play vital roles in cell adhesion, cell metabolism, and thyroxine synthesis. Combining patient clinical trial sample information, we further compared the transient changes of gene expression regulation in HT and papillary thyroid carcinoma (PTC) samples. More importantly, we developed patient-derived HT and PTC organoids as a promising new preclinical model to verify these potential markers. Our data revealed a marked characteristic of HT organoid in upregulating chemokines that include C-C motif chemokine ligand (CCL) 2 and CCL3, which play a key role in the pathogenesis of HT. Overall, our research has enriched everyone’s understanding of the pathogenesis of HT and provides a certain reference for the treatment of the disease. Frontiers Media S.A. 2021-12-02 /pmc/articles/PMC8674930/ /pubmed/34925365 http://dx.doi.org/10.3389/fimmu.2021.784975 Text en Copyright © 2021 Xiao, Liang, Liu, Zhang, Xie, Kong, Guo, Wang, Fu, Ye, Li, Zhang, Zhang, Kaudimba, Wang, Kong, Zhao, Zheng and Liu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Xiao, Hui
Liang, Jianqing
Liu, Sunqiang
Zhang, Qiongyue
Xie, Famin
Kong, Xingyu
Guo, Shanshan
Wang, Ruwen
Fu, Rong
Ye, Zhiqi
Li, Yun
Zhang, Shuang
Zhang, Li
Kaudimba, Keneilwe Kenny
Wang, Ru
Kong, Xingxing
Zhao, Bing
Zheng, Xuqin
Liu, Tiemin
Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis
title Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis
title_full Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis
title_fullStr Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis
title_full_unstemmed Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis
title_short Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis
title_sort proteomics and organoid culture reveal the underlying pathogenesis of hashimoto’s thyroiditis
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8674930/
https://www.ncbi.nlm.nih.gov/pubmed/34925365
http://dx.doi.org/10.3389/fimmu.2021.784975
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